褪黑素干预急性坏死性胰腺炎肺损伤及巨噬细胞移动抑制因子表达

中国临床药理学与治疗学 ›› 2009, Vol. 14 ›› Issue (3): 261-264.

褪黑素干预急性坏死性胰腺炎肺损伤及巨噬细胞移动抑制因子表达

董乐妹, 吴建胜, 贾国葆, 方佩佩, 孙学成, 倪银

温州医学院附属第一医院消化内科, 温州325000, 浙江

收稿日期:2008-09-25 修回日期:2008-09-25 发布日期:2020-10-27
通讯作者: 吴建胜, 男, 主任医师, 硕士生导师, 主要从事消化病学研究。Tel:13705880087
作者简介:董乐妹, 女, 在读硕士研究生, 主要从事消化病学研究。Tel:13758497528 E-mail:donglemei2006@126.com
基金资助:
温州市科技计划项目(Y20060068)

Effects of melatonin on acute necotizing pancreatitis associated lung injury and the expression of macrophage migration inhibition factor

DONG Le-mei, WU Jian-sheng, JIA Guo-bao, FANG Pei-pei, SUN Xue-cheng, NI Yin

Department of Gastroenterology, the First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, Zhejiang, China

Received:2008-09-25 Revised:2008-09-25 Published:2020-10-27

摘要/Abstract

摘要： 目的研究急性坏死性胰腺炎(acute necrotizing pancreatitis,ANP)大鼠肺组织巨噬细胞移动抑制因子(MIF)、TNF-α的变化及褪黑素(MT)的干预效果。方法 36 只大鼠随机分为假手术组(SO组)、急性坏死性胰腺炎组(ANP 组)和MT 干预组(MT 组), 每组12 只。应用胰胆管内注射牛磺胆酸钠的方法诱导ANP 模型,MT 组于诱导模型前30 min 皮下注射MT。术后4 h 和24 h 分批处死大鼠(每个时间点n =6), 用RT-PCR 检测肺组织MIF 、TNF-αmRNA 的表达情况, 用SABC 免疫组织化学法检测肺脏MIF 蛋白表达情况, 并行胰腺及肺脏的病理检查。结果 ANP 组4 、24 h 肺组织MIF 、TNF-αmRNA 表达较SO 组明显升高(P<0.05), 肺脏及胰腺病理损伤较严重;MT 组较ANP组肺组织MIF 、TNF-αmRNA 表达下降(P<0.05),病理损伤减轻;MIF 主要表达于肺脏支气管上皮细胞胞浆。结论 MIF 可能参与急性胰腺炎肺损伤的发生,MT 可减弱其肺内表达及胰腺炎相关肺损伤的严重程度。

关键词: 急性坏死性胰腺炎, 肺损伤, 巨噬细胞移动抑制因子, 褪黑素

Abstract: AIM: To clarify the effects of melatonin on acute necrotizing pancreatitis (ANP)associated lung injury and the expressions of macrophage migration inhibition factor (MIF)and TNF-αin the lung of rats. METHODS: A total of 36 SD rats were randomly divided into sham operation group(SO), acute necrotizing pancreatitis group (ANP) and melatonin group (MT).ANP was induced by the injection of sodium taurocholate into the pancreatic duct.Rats inMT group were injected intraperitoneally with MT 30 min before the induction of ANP.Rats were sacrificed at 4 and 24 hours after the induction of ANP(n =6 for each time point).RT-PCR was used to measure the mRNA levels of MIF and TNF-αin the lung.Immunohistochemistry analysis was performed on the lung tissues to detect MIF.The histopathological changes of the pancrea and the lung were also observed. RESULTS: Compared with the SO group. the pancreatic and pulmonary pathological changes in ANP group and MT group were significantly aggravated. the mRNA levels of MIF and TNF-αin the lung were markedly up-regulated(P<0.05), Compared with the ANP group. the pulmonary and pancreatic pathological changes in MT group were alleviated. the mRNA levels of MIF and TNF-αin the lung of MT group were decreased significantly (P<0.05).Positive immunostaining for MIF was observed predominantly within the bronchial epithelial cells in the lung. CONCLUSION: MIF may play an important role in the pathogenesis of ANP associated lung injury. MT can reduce its expression and alleviate the lung injury.

Key words: acute necrotizing pancreatitis, lunginjury, macrophage migration inhibition factor, melatonin

中图分类号:

R965.2

董乐妹, 吴建胜, 贾国葆, 方佩佩, 孙学成, 倪银. 褪黑素干预急性坏死性胰腺炎肺损伤及巨噬细胞移动抑制因子表达[J]. 中国临床药理学与治疗学, 2009, 14(3): 261-264.

DONG Le-mei, WU Jian-sheng, JIA Guo-bao, FANG Pei-pei, SUN Xue-cheng, NI Yin. Effects of melatonin on acute necotizing pancreatitis associated lung injury and the expression of macrophage migration inhibition factor[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(3): 261-264.

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