红霉素对哮喘大鼠核因子NF-κB 及Th2 类细胞因子的影响

中国临床药理学与治疗学 ›› 2009, Vol. 14 ›› Issue (3): 292-295.

红霉素对哮喘大鼠核因子NF-κB 及Th2 类细胞因子的影响

张近波¹, 吴成云², 李智强¹, 戴元荣²

¹温州医学院附属第一医院ICU, 温州325000, 浙江;
²温州医学院附属第二医院呼吸内科, 温州325000, 浙江

收稿日期:2008-11-08 修回日期:2008-11-08 发布日期:2020-10-27
通讯作者: 吴成云,男, 硕士研究生, 副主任医师, 主要从事慢性阻塞性肺疾病方面的研究。E-mail:w cyzxqdd @hotmail.com
作者简介:张近波, 男, 硕士研究生, 住院医师, 主要从事危重病方面的研究。Tel:13757728837 E-mail:zhangjinbo0661 @sina.com
基金资助:
温州市科学技术局资助项目(Y2006A036)

Effects of erycin on NF-κB and Th2 in rats with asthma

ZHANG Jin-bo¹, WU Cheng-yun², LI Zhi-qiang¹, DAI Yuan-rong²

¹First Affiliated Hospital, Wenzhou Medical College;
²Second Affiliated Hospital, Wenzhou Medical College,Wenzhou 325000, Zhejiang, China

Received:2008-11-08 Revised:2008-11-08 Published:2020-10-27

摘要/Abstract

摘要： 目的探讨红霉素对哮喘大鼠NF-κB 、Th2类细胞因子的影响。方法分离、培养正常对照组、哮喘组、红霉素处理组大鼠的外周血单核细胞(PBMCs)。分别采用ELISA 法检测细胞上清液中IL-4 、IL-5 、IL-13 浓度。逆转录聚合酶链反应(RTPCR)、Western blotting 技术检测PBMCs NF-κB mRNA、NF-κB 蛋白的表达。结果哮喘组PBMCs mRNA、NF-κB 蛋白表达水平及细胞上清液中IL-4 、IL-5 、IL-13 的浓度较正常对照组显著升高(P<0.05), 红霉素处理组NF-κB mRNA 、NF-κB 蛋白表达水平及细胞上清液中IL-4 、IL-5 、IL-13 的浓度较哮喘组显著降低(P<0.05)。通过直线相关分析发现, PBMCs NF-κB 蛋白与细胞上清液中IL-4 、IL-5 、IL-13 的表达水平之间均呈显著正相关(r 分别为0.78 、0.86 、0.71, P 均<0.05)。结论 NF-κB可能参与支气管哮喘的病理生理过程。红霉素可能通过作用于NF-κB 这一靶点发挥其免疫调节作用。

关键词: 哮喘, 外周血单个核细胞, NF-κB, 红霉素

Abstract: AIM: To explore the effects of erycin on the expression of cytokines such as NF-κB and Th2 in asthmatic rats. METHODS: The peripheral blood monouclear cells(PBMCs) were separated and cultured from rats in bronchial asthma, normal control and erycin-treatment groups.The concentrations of IL-4, IL-5 and IL-13 in plasma, the expressions of NF-κB mRNA and NF-κB protein in PBMCs were detected by enzyme linked immunosorbent assay (ELISA), reverse transcription polymerase chain reaction (RT-PCR), Western blotting, respectively. RESULTS: Compared with normal group the expressions of NF-κB mRNA and NF-κB protein in PBMCs and the concentrations of IL-4, IL-5 and IL-13 in supernatant were higher in asthmatic group (P<0.05).Compared with the asthmatic group, the above indexes were decreased significantly in erycin-treatment group (P<0.05).There were positive correlations between the expression of NF-κB protein in PBMCs and the concentrations of IL-4, IL-5 and IL-13 in supernatant (r =0.78, 0.86 and 0.71, P<0.05). CONCLUSION: NF-κB may play a role in patho-physiological course of asthma.Erycin could effectively treat asthma by inhibiting the expression of NF-κB.

Key words: asthma, peripheral blood mononuclear cell, NF-κB, erycin

中图分类号:

R965.2

张近波, 吴成云, 李智强, 戴元荣. 红霉素对哮喘大鼠核因子NF-κB 及Th2 类细胞因子的影响[J]. 中国临床药理学与治疗学, 2009, 14(3): 292-295.

ZHANG Jin-bo, WU Cheng-yun, LI Zhi-qiang1, DAI Yuan-rong. Effects of erycin on NF-κB and Th2 in rats with asthma[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(3): 292-295.

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