卡介苗对毛细支气管炎患儿免疫功能的影响

中国临床药理学与治疗学 ›› 2009, Vol. 14 ›› Issue (3): 342-345.

卡介苗对毛细支气管炎患儿免疫功能的影响

黄晖, 刘雅丽

杭州市第一人民医院儿科, 杭州300016, 浙江

收稿日期:2008-10-15 修回日期:2008-10-15 发布日期:2020-10-27
作者简介:黄晖, 女, 副主任医师, 研究方向:小儿呼吸。Tel:13588807906 E-mail:Hzhh678 @hotmail.com

Effects of Bacille Calmette Guerin on immunological function of infants with bronchiolitis

HUANG Hui, LIU Ya-li

Department of Paediatrics, the First Hospital of Hangzhou, Hangzhou 300016, Zhejiang, China

Received:2008-10-15 Revised:2008-10-15 Published:2020-10-27

摘要/Abstract

摘要： 目的探讨卡介苗体外对毛细支气管炎患儿免疫功能的影响。方法取健康及毛细支气管炎患儿外周血体外分离单核细胞, 加入不同浓度的卡介苗(BCG) 培养, 观察BCG 对IL-12 、IL-10 水平的影响;同时分离外周血的淋巴细胞, 加入经BCG 处理的单核细胞培养液, 用生理盐水作为阴性对照, 培养满3 d 收集培养上清液, 检测细胞因子IL-4 水平。结果体外实验结果显示BCG 刺激单核细胞分泌的细胞因子水平(IL-10 、IL-12) 在一定浓度下呈浓度依赖性, BCG 浓度为50 μL mL时, 单核细胞分泌的细胞因子IL-10 水平最高;IL-4 的水平下降, 与干预前相比具有统计学差异;IL-12 升高不显著。结论适宜浓度的BCG 具有增强TH1 的作用, IL-4 水平降低推测可能与IL-10 诱导产生调节性T 细胞(CD4 +CD25 +Tr) 有关。

关键词: 毛细支气管炎, 卡介苗, 白细胞介素-4, 白细胞介素-10, 白细胞介素-12

Abstract: AIM: To approach the effects of Bacille Calmette-Guerin(BCG) on immunological function of infants with bronchiolitis in vitro. METHODS: The monocytes were segregated from the peripheral blood of infants with acute bronchiolitis and healthy infants as control.The cytokines of IL-10 and IL-12 were measured by ELISA after interfered with different concentrations of BCG in vitro.IL-4, produced by lymphocytes treated with the cultural supernatant of BCG-stimulated monocytes were determined by ELISA.Acute bronchiolitis infants and health children treated with normal saline were as control. RESULTS: BCG stimulated IL-10 and IL-12 production in a dose response manner in a certain concentration range.The higher level of IL-10 was observed when BCG concentration reached 50 μL mL, while IL-12 had no significant increase.The level of IL-4 was markedly decreased compared with pre-treatment (P<0.05). CONCLUSION: Proper concentrations of BCG could induce a TH1 cytokine response effectively, the level of IL-4 was degraded after BCG intervention, presuming concerned with regular T cells(CD4 +CD25 +Tr) induced by IL-10.

Key words: bronchiolitis, Bacille Calmette-Guerin, IL-4, IL-10, IL-12

中图分类号:

R725.6

黄晖, 刘雅丽. 卡介苗对毛细支气管炎患儿免疫功能的影响[J]. 中国临床药理学与治疗学, 2009, 14(3): 342-345.

HUANG Hui, LIU Ya-li. Effects of Bacille Calmette Guerin on immunological function of infants with bronchiolitis[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(3): 342-345.

参考文献

[1] 刘雅丽, 扬锡强.毛细支气管炎患儿免疫功能状态的研究[J].实用儿科临床杂志, 2006, 6(10):584-586.
[2] Choi IS, Koh YI.Therapeutic effects of BCG vaccination in adult asthmatic patients:a randomized controlled trial [J].Ann Allergy Asthma Immunol, 2002, 88(6):584-591.
[3] Shirakawa T, Enomoto T, Shimazu S, et al.The inverse association between tuberculin responses and atopic disorder[J].Science, 1997, 275(5296):77-79.
[4] Von Mutius, Pearce P, Beaseley R, et al.International patterns of tuberculosis and the prevalence of symptoms of asthma, rhinitis, and ecazema[J].Thorax, 2000, 55(6): 440-453.
[5] Scanga CB, Le Gros G.Development of an asthma vaccine research into BCG[J].Drugs, 2000, 59(6):1217-1221.
[6] Koh YI, Choi IS, Kim WY.BCG infection in allergen-presensitized rats suppresses Th2 immune response and prevents the development of allergic asthmatic reaction[J].J Clin Immunol, 2001, 21(1):51-59.
[7] Choi IS, Koh YI.Therapeutic effects of BCG vaccination in adult asthmatic patients:a randomized, controlled trial [J].Ann Allergy Asthma Immunol, 2002, 88(6):584-591.
[8] Choi IS, Koh YI.Effects of BCG revaccination on asthma [J].Allergy, 2003, 58(11):1114-1116.
[9] Shirtcliffe PM, Easthope SE, Cheng S, et al.The effect of delipidated deglycolipidated (DDMV) and heat-killed My cobacterium vaccae in asthma[J].Am J Respir Crit Care Med, 2001, 163(6):1410-1414.
[10] 刘雅丽, 扬锡强.免疫治疗对毛细支气管炎后哮喘发生的干预机制研究[J].中国临床药理学与治疗学,2006, 11(9):1-5.
[11] Jeffrey AB, Abul KA.Natural versus adaptive regulatory T cells[J].Nat Rev Immunol, 2003, 3(3):253-257.
[12] Taylor A, Verhagen J, Akdis CA, et al.T regulatory cells in allergy and health:a question of allergen specificity and balance[J].Int Arch Allergy Immunol, 2004, 135(1):73-82.

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