丹参酮ⅡA 纳米制剂对大鼠全脑缺血再灌注损伤的保护作用

中国临床药理学与治疗学 ›› 2009, Vol. 14 ›› Issue (4): 391-396.

丹参酮ⅡA 纳米制剂对大鼠全脑缺血再灌注损伤的保护作用

陈峰¹, 叶龙彬², 辛中帅², 奚涛¹

¹中国药科大学分析测试中心电镜室, ²中国药科大学生命科学与技术学院, 南京210009, 江苏

收稿日期:2009-04-15 修回日期:2009-02-13 发布日期:2020-10-29
通讯作者: 奚涛,男,教授,博士研究生导师,研究方向:药理学与生物技术。Tel:025-83271389 E-mail:xitao18@sina.com
作者简介:陈峰,男,助理研究员,研究方向:转基因与分子药理学。Tel:025-85391263 E-mail:dimple292@sina.com

Protective effect of preparation of nanometer tanshinone ⅡA against global cerebral ischemia-reperfusion injury in rats

CHEN Feng¹, YE Long-bin², XIN Zhong-suai², XI Tao²

¹Electron Microscopy Lab,²College of Science and Technology, China Pharmaceutical University, Nanjing 210009,Jiangsu, China

Received:2009-04-15 Revised:2009-02-13 Published:2020-10-29

摘要/Abstract

摘要： 目的: 观察丹参酮ⅡA纳米制剂对大鼠全脑缺血再灌注损伤的保护作用。方法: 采用三动脉夹闭法制备大鼠全脑缺血再灌注损伤模型,以丹参酮ⅡA纳米制剂灌胃给药,观察丹参酮ⅡA纳米制剂对脑组织的丙二醛(MDA)含量、超氧化物歧化酶(SOD)活力和脑组织Na⁺-K⁺-ATPase活性水平的影响。做脑组织病理切片检查和透射电镜观察。结果: 丹参酮ⅡA纳米制剂(25mg/kg)能使脑组织MDA含量下降(P<0.01),SOD活性明显升高(P<0.05),Na⁺-K⁺-ATP活性显著上升(P<0.01),病理切片和透射电镜观察显示丹参酮ⅡA纳米制剂能在不同的水平上抑制细胞和线粒体的损伤。结论: 丹参酮ⅡA纳米制剂对全脑缺血再灌注损伤具有很好的保护作用。

关键词: 丹参酮ⅡA 纳米制剂, 全脑缺血再灌注, 自由基, Na⁺-K⁺-ATP 酶, 细胞超微形态

Abstract: AIM: To investigate the protection effect of preparation of nanometer Tanshinone ⅡA against brain injury in rats undergone global cerebral ischemia reperfusion.METHODS: The rats were intragastrically administrated with the preparation of nanometer Tanshinone ⅡA , then the global cerebral ischemia-reperfusion model of rat was made by clipping and releasing three arteries.The content of MDA and the activity of SOD and Na⁺-K⁺-ATPase in homogenate of the cerebral tissues were measured.The sections of pathology and ultrastructure of mitochondrion in cortex had been observed.RESULTS: Tanshinone ⅡA (25 mg/kg ) could obviously decrease the content of MDA (P<0.01)and increase the activity of SOD(P<0.05) and Na⁺-K⁺-ATPase(P<0.05)in rats brain tissue.The observation of pathology and ultrastructure showed that the injury of cell and mitochondrion had been restrained in these of rat brain.CONCLUSION: Preparation of nanometer Tanshinone ⅡA has protective effect against global cerebra injury caused by global cerebral ischemia-reperfusion injury in rats.

Key words: preparation of nanometer tanshinone ⅡA, global cerebral ischemia-reperfusion injury, free radicals, Na⁺-K⁺-ATPase, ultrastructure of cell

中图分类号:

R965.2

陈峰, 叶龙彬, 辛中帅, 奚涛. 丹参酮ⅡA 纳米制剂对大鼠全脑缺血再灌注损伤的保护作用[J]. 中国临床药理学与治疗学, 2009, 14(4): 391-396.

CHEN Feng, YE Long-bin, XIN Zhong-suai, XI Tao. Protective effect of preparation of nanometer tanshinone ⅡA against global cerebral ischemia-reperfusion injury in rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(4): 391-396.

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