中重度哮喘急性发作患儿血中EAN-78 的表达及甲基强的松龙对其表达的影响

中国临床药理学与治疗学 ›› 2009, Vol. 14 ›› Issue (5): 564-566.

中重度哮喘急性发作患儿血中EAN-78 的表达及甲基强的松龙对其表达的影响

金小红¹, 陈存国¹, 李凤仙¹, 李绍波¹, 陈丽丽¹, 陈保国¹, 童夏生²

¹温州医学院附属台州医院, 临海317000, 浙江;
²浙江省台州中西医结合医院, 温岭317523, 浙江

收稿日期:2009-03-04 修回日期:2009-05-12 发布日期:2020-11-09
通讯作者: 童夏生, 男, 硕士, 副主任医师, 研究方向:儿童呼吸。Tel:13758688128 E-mail:xshtzg@163. com
作者简介:金小红, 女, 硕士, 副主任医师, 研究方向:儿科呼吸和哮喘。Tel:13867619616 E-mail:jinxhtz @163. com

Expressions of epithelial cell-derived neutrophil-activating protein-78 in moderate and severe acute asthma children and the influence of methylprednisolone

JIN Xiao-hong¹, CHEN Cun-guo¹, LI Feng-xian¹, LI Shao-bo¹, CHEN Li-li¹, CHEN Bao-guo¹, TONG Xia-shen²

¹Taizhou Hospital, Affiliated Wenzhou Medical College, Linhai 317000, Zhejiang, China;
²Taizhou Integrated Traditional and Western Medicine Hospital, Wenling 317523, Zhejiang, China

Received:2009-03-04 Revised:2009-05-12 Published:2020-11-09

摘要/Abstract

摘要： 目的:观察中重度哮喘急性发作患儿血中内皮源性中性粒细胞激活肽-78(EAN-78) 的表达水平及甲基强的松龙(甲强龙) 对其的影响。方法:选择中重度哮喘急性发作患儿为观察对象, 分为甲强龙干预前、后组, 小儿外科斜疝患儿(无哮喘病史) 为对照组, 留取外周血标本检测中性粒细胞(PMN) 数目, 并采用流式细胞术检测EAN-78 的含量。结果:观察组血ENA-78 水平明显高于对照组(P <0. 01), 其中干预前组血ENA-78 水平又明显高于干预后组(P <0. 01); 观察组外周血PMN 均分别较对照组明显增高, 差异有统计学意义(P <0. 01), 但干预前、后两组外周血PMN 计数比较差异无统计学意义(P >0. 05); 甲强龙干预前组血ENA-78 的表达与PMN 数目呈显著正相关(n =32, r =0. 865, P <0. 01) 。结论:中重度哮喘急性发作时患儿血中ENA-78 的表达及PMN 数量是升高的, 它们可能共同参与了哮喘发病的炎症过程;甲强龙部分通过抑制ENA-78 的表达来减轻气道炎症。

关键词: 哮喘, 内皮源性中性粒细胞激活肽-78, 中性粒细胞, 甲强龙

Abstract: AIM:To observe the expressions of epithelial cell-derived neutrophil-activating protein-78 in acute asthma of severe cases and the influence of methylprednisolone. METHODS:Children with moderate and severe acute asthmawere divided into urbason pre-interfere group, post-interfere group, and the pediatric surgery indirect hernia children as control group (without asthma case history), the numbers of heterophil granulocyte(PMN) in peripheral blood preparation were detected, and the expressions of EAN-78 protein were measured by flow cytometry. RESULTS:Compared with the control group, the levels of ENA-78 protein in asthma group were higher(P <0. 01). Compared with post-interfere group, the levels of ENA-78 protein in pre-interfere group were significantly higher (P <0. 01). Compare with the control group, the numbers of peripheral blood PMN in pre-interfere and post-interfere group were increased, there were significant differences(P <0. 01), and there were no significant differences about the numbers of PMN between pre-interfere and post-interfere group (P >0. 05). There was a positive correlation between the levels of ENA-78 protein and the numbers of PMN by methylprednisolone (n =32, r =0. 865, P <0. 01). CONCLUSION: The levels of ENA-78 protein and the numbers of PMN in blood are increased in moderate and severe acute asthma children. They may be involved in the inflammation process of asthma pathogenesis. Methylprednisolone can relieve the airway inflammation by inhibiting the expressions of ENA-78 protein.

Key words: asthma, epithelial cell-derived neutrophil-activating protein-78, heterophil granulocyte, methylprednisolone

中图分类号:

R472. 9

金小红, 陈存国, 李凤仙, 李绍波, 陈丽丽, 陈保国, 童夏生. 中重度哮喘急性发作患儿血中EAN-78 的表达及甲基强的松龙对其表达的影响[J]. 中国临床药理学与治疗学, 2009, 14(5): 564-566.

JIN Xiao-hong, CHEN Cun-guo, LI Feng-xian, LI Shao-bo, CHEN Li-li, CHEN Bao-guo, TONG Xia-shen. Expressions of epithelial cell-derived neutrophil-activating protein-78 in moderate and severe acute asthma children and the influence of methylprednisolone[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(5): 564-566.

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