新型表位基因疫苗的构建及对小鼠黑色素瘤的影响

中国临床药理学与治疗学 ›› 2009, Vol. 14 ›› Issue (7): 785-789.

新型表位基因疫苗的构建及对小鼠黑色素瘤的影响

商明红¹, 林森森², 孙立², 张彦凯¹, 袁胜涛², 张陆勇¹

¹中国药科大学新药筛选中心, ²江苏省药效学研究与评价服务中心, 南京210038, 江苏

收稿日期:2009-04-06 修回日期:2009-04-30 出版日期:2009-07-26 发布日期:2020-10-30
通讯作者: 张陆勇, 男, 教授, 博士研究生导师, 研究方向:分子药理学。Tel:025-85391056 　E-mail:drugscreen@126.com;袁胜涛, 男, 副研究员, 硕士研究生导师, 研究方向:肿瘤药理学。Tel:025-85391036 　E-mail:yuanst2@yahoo.com.cn
作者简介:商明红, 男, 硕士研究生, 研究方向:肿瘤免疫学。Tel:13851937607 　E-mail:smhzzz@126.com
基金资助:
江苏省药效研究和评价服务中心资助项目(BM2005103)

Suppressing effect of a novel epitopes DNA vaccine on mouse melanoma growth

SHANG Ming-hong¹, LIN Sen-sen², SUN Li², ZHANG Yan-kai¹, YUAN Sheng-tao², ZHANG Lu-yong¹

¹New Drug Screening Center, China Pharmaceutical University, ²Jiangsu Service Center for Pharmacodynamics Research and Evaluation, Nanjing 210038, Jiangsu, China

Received:2009-04-06 Revised:2009-04-30 Online:2009-07-26 Published:2020-10-30

摘要/Abstract

摘要： 目的:构建以MAGE-1(161-169) 为表位的癌症基因疫苗并检测其抗小鼠黑色素瘤效果。方法:构建基因疫苗pcDNA-HSP70-MAGE-1(161-169),并免疫C57BL 6 小鼠。最后一次免疫后第2 周,接种小鼠黑色素瘤细胞。于肿瘤细胞接种后14 d, 处死全部动物, 称量肿瘤的重量。采用ELISA 法对小鼠血清中抗MAGE-1-IgG 类抗体及小鼠原代脾淋巴细胞IFN-γ释放水平进行检测。结果:pcDNA-HSP70-MAGE-1(161-169) 表位基因疫苗能够成功地诱发机体产生抗MAGE-1 的特异性抗体, 并能在体内起到抗小鼠黑色素瘤作用, 抑瘤率为40.7%。同时还能提高约3.05 倍的小鼠原代脾淋巴细胞IFN-γ释放量。结论:pcDNA-HSP70-MAGE-1(161-169) 有望成为有效的抗小鼠黑色素瘤基因疫苗。

关键词: 基因疫苗, 免疫原性, 小鼠黑色素瘤

Abstract: AIM: Construction of MAGE-1(161-169) DNA vaccine and evaluated its anti-melanoma effects in mouse.METHODS: A DNA vaccine pcDNA3.1-HSP70-MAGE-1(161-169) has been constructed and used to immunize mice 3 times at 2-weekly intervals.Two weeks after the last immunization, tumor challenge experiments were performed by using B16F10 melanoma cell.After 14 d of challenge experiments, all mice were sacrificed and tumors were weighted.The specific anti-MAGE-1 IgG antibodies and the IFN-γreleased by mouse primary splenocytes were detected by ELISA methods.RESULTS: The specific anti-MAGE-1 antibodies were detected in the serum of the mice immunized with pcDNA3.1-HSP70-MAGE-1(161-169) DNA vaccine.It showed that B16F10 melanoma growth in mice of DNA vaccine group was obviously suppressed compared with that in saline control group, with tumor inhibitory rate of 40.7%.The IFN-γ released by mouse primary splenocytes in mice of HSP70-MAGE-1(161-169) DNA vaccine group was 3.05 times of that in saline control group.CONCLUSION: pcDNA3.1-HSP70-MAGE-1(161-169) may be an effective DNA vaccine for the treatment of MAGE-1 dependent tumors.

Key words: DNA vaccine, immunogenicity, mouse melanoma

中图分类号:

R965.2

商明红, 林森森, 孙立, 张彦凯, 袁胜涛, 张陆勇. 新型表位基因疫苗的构建及对小鼠黑色素瘤的影响[J]. 中国临床药理学与治疗学, 2009, 14(7): 785-789.

SHANG Ming-hong, LIN Sen-sen, SUN Li, ZHANG Yan-kai, YUAN Sheng-tao, ZHANG Lu-yong. Suppressing effect of a novel epitopes DNA vaccine on mouse melanoma growth[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(7): 785-789.

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