雄黄微生物提取液对 K562/ADM 细胞P-糖蛋白和多药耐药基因表达的影响

中国临床药理学与治疗学 ›› 2009, Vol. 14 ›› Issue (8): 855-860.

雄黄微生物提取液对 K562/ADM 细胞P-糖蛋白和多药耐药基因表达的影响

张景红^1,2, 李红玉¹

¹兰州大学生命学院,兰州 730000, 甘肃;
²华侨大学分子药物教育部工程研究中心,泉州 362021,福建

收稿日期:2009-03-31 修回日期:2009-07-14 出版日期:2009-08-26 发布日期:2020-10-30
作者简介:张景红, 女, 副教授, 硕士生导师, 研究方向:中药生物工程和肿瘤免疫学。Tel:13506922986 E-mail:zjh@hqu.edu.cn
基金资助:
甘肃省科技攻关项目(2GS064-A43-019-02); 福建省自然科学基金项目(2008J0102); 泉州市基金项目(2008Z21)

Effects of realgar bioleaching solution on the expression of MDRI mRNA and P-gp in K562/ADM cells

ZHANG Jing-hong^1,2, LI Hong-yu¹

¹Life Science, Lanzhou University, Lanzhou 730000, Gansu, China;
²Molecular Medicine Engineering Research Central Ministry of Education, Huaqiao University, Quanzhou362021, Fujian, China

Received:2009-03-31 Revised:2009-07-14 Online:2009-08-26 Published:2020-10-30

摘要/Abstract

摘要： 目的: 研究雄黄微生物提取液(RBS)诱导K562/ADM 细胞凋亡的作用, 并探讨其对P-糖蛋白(P-gp)和多药耐药基因(MDRI)表达的影响。方法: 采用 “微生物浸出” 法制备RBS, 并以H₃AsO₃ 作为阳性对照,AnnexinV-PI 双染法检测细胞凋亡;流式细胞仪检测细胞P-gp表达率;逆转录聚合酶链技术(RT-PCR)检测细胞 MDRI mRNA表达水平。结果: RBS 可诱导多药耐药白血病细胞发生典型凋亡,抑制 P-gp 的表达, 下调 MDRI mRNA表达水平。在含砷量相同的条件下, RBS诱导效应明显强于 H₃AsO₃。结论: 诱导细胞凋亡、下调P-gp/MDRI 蛋白的表达,可能是雄黄逆转白血病耐药的重要分子机制。

关键词: 雄黄微生物提取液, 耐药白血病细胞, 凋亡, P-糖蛋白, 多药耐药基因

Abstract: AIM: To investigate the role of apop-tosis in K562/ADM cells induced by realgar bioleaching solution (RBS), and illustrate the possible molecular mechanism .METHODS: RBS was prepared by a new method of bioleaching with bacteria .The cell apoptosis was determined by annexin V/PI double staining .The expressions of MDRI mRNA and P-gp were detected by RT-PCRand flow cytometry, respectively.The parallel experiments with arsenic acid (H₃AsO₃)were conducted for comparison .RESULTS: RBS inhibited K562/ADM cells growth effectively, and the apoptosis rate of the cells by Annexin V/PI staining was obviously in-creased, the expressions of MDRI mRNA and P-gp were significantly down-regulated. With the same con-centration of As, the inducing effect of RBS was more stronger than H₃AsO₃ .CONCLUSION: RBS induces the apoptosis inK562/ADM cells. The down-regulation of MDRI P-gp expression may be the important molecular mechanisms in reversing the multi-drug resistance leukemia cells.

Key words: realgar bioleaching solution, K562/ADM cell, apoptosis, P-gp, MDRI

中图分类号:

R965.2

张景红, 李红玉. 雄黄微生物提取液对 K562/ADM 细胞P-糖蛋白和多药耐药基因表达的影响[J]. 中国临床药理学与治疗学, 2009, 14(8): 855-860.

ZHANG Jing-hong, LI Hong-yu. Effects of realgar bioleaching solution on the expression of MDRI mRNA and P-gp in K562/ADM cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(8): 855-860.

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