高效液相色谱柱前衍生化方法分析血浆中3-O甲基葡萄糖

中国临床药理学与治疗学 ›› 2010, Vol. 15 ›› Issue (11): 1245-1250.

高效液相色谱柱前衍生化方法分析血浆中3-O甲基葡萄糖

M'boungou Gwladys Jysmard, 王新廷, 刘晓东

中国药科大学药物代谢动力学重点实验室,南京210009,江苏

收稿日期:2010-07-14 修回日期:2010-08-12 出版日期:2010-11-26 发布日期:2020-09-16

The assay of the 3-o-methyl-d-glucose (3-OMG) in plasma with derivatization method by High Performance Liquid Chromatography

M'boungou Gwladys Jysmard, WANG Xin-ting, LIU Xiao-dong

Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing 210009, Jiangsu, China

Received:2010-07-14 Revised:2010-08-12 Online:2010-11-26 Published:2020-09-16
Contact: LIU Xiao-dong, professor, dedicated to the research of pharmacokinetic lesearch of new drug, drug transport and blood brain barrier, pharmacokinetics of herbal medicine. Tel: 025-83271006 E-mail: xdliu@cpu.edu.cn
About author:M'boungou Gwladys Jysmard, graduate student from the Republic of Gongo, dedicated to the pharmacokinetics research. E-mail: mboungougwla@yahoo.fr
Supported by:
This work is supported by the National Science Foundation of People's Republic of China(Grants 30873123, 81072693) and the Fundamental Research Funds for the Central Universities(Grant JKY2009009)

摘要/Abstract

摘要： 目的: 本文首次建立了用1-苯基-3-甲基-5-吡唑啉酮(PMP)作为柱前衍生化试剂对3-O-甲基-葡萄糖(3-OMG)进行衍生化的方法,并应用此方法对大鼠血浆中的3-OMG进行分析。方法: 70 ℃水浴时,3-OMG在 0.4 mol/L的NaOH条件下和PMP进行反应。衍生物采用反相高效液相色谱法进行分析,紫外检测波长为 245 nm。结果: 线性系数在 0.991 以上,最低定量限 0.0078 mg/mL。回收率在98%~105%之间,且日间与日内变异不高于12%。本方法成功应用于糖尿病大鼠血浆中3-OMG的分析。结论: 本方法简便、高效,适合对正常和糖尿病大鼠的血浆3-OMG进行分析。

关键词: 3-O-甲基-葡萄糖, 1-苯基-3-甲基-5-吡唑啉酮, 衍生化

Abstract: AIM: To establish a convenient method to analyze 3-O-Methyl-D-Glucose (3-OMG) in rat plasma using 1-phenyl-3-methyl-5-pyrazolone (PMP) as a pre-column derivatizing agent, and in the mean time using this method to analyze the 3-OMG in rat plasma. METHODS: 3-OMG reacted easily with PMP in the presence of 0.4 mol/L NaOH at a 70 ℃ water bath. The derivatives were analyzed by Reverse Phase High Performance Liquid Chromatography (RP-HPLC) and monitored by ultraviolet detector at 245 nm. RESULTS: All linear coefficients were over 0.991, and the quantitative limit was 0.0078 mg/mL. The recoveries were found to be 98%-105%. Variations of intra- and inter-day precision were less than 12%. The method was successfully applied to the analysis of 3-OMG in rat plasma. CONCLUSION: This method is simple and effective, and can be adopted to analyze the 3-OMG in the plasma from both normal and diabetic rats.

Key words: 3-O-methyl-glucose, 1-phenyl-3-methyl-5-pyrazolone, Derivatization

中图分类号:

R969.1

M'boungou Gwladys Jysmard, 王新廷, 刘晓东. 高效液相色谱柱前衍生化方法分析血浆中3-O甲基葡萄糖[J]. 中国临床药理学与治疗学, 2010, 15(11): 1245-1250.

M'boungou Gwladys Jysmard, WANG Xin-ting, LIU Xiao-dong. The assay of the 3-o-methyl-d-glucose (3-OMG) in plasma with derivatization method by High Performance Liquid Chromatography[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(11): 1245-1250.

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