组胺对白色脂肪细胞脂肪合成以及调控的机制研究

中国临床药理学与治疗学 ›› 2010, Vol. 15 ›› Issue (12): 1343-1347.

组胺对白色脂肪细胞脂肪合成以及调控的机制研究

曾慧¹, 刘昭前²

¹长沙市卫生学校,长沙 410100,湖南;
²中南大学临床药理研究所,长沙 410078,湖南

收稿日期:2010-09-26 修回日期:2010-11-19 出版日期:2010-12-26 发布日期:2020-09-16
通讯作者: 刘昭前,男,教授,研究方向:遗传药理学。Tel: 0731-4805380 E-mail: liuzhaoqian63@126.com
作者简介:曾慧,女,硕士研究生,讲师,研究方向:肥胖症和2型糖尿病的药物基因组学研究。Tel: 13755006146 E-mail: zenghui421@sina.com

Effect of histamine on fat synthesis and regulation in the white adipocytes: a mechanism research

ZENG Hui¹, LIU Zhao-qian²

¹Changsha Health School , Changsha 410100,Hunan,China;
²Clinical Pharmacology Institute of Centre South University, Changsha 410078, Hunan, China

Received:2010-09-26 Revised:2010-11-19 Online:2010-12-26 Published:2020-09-16

摘要/Abstract

摘要： 目的: 在体外探索组胺对脂肪合成的影响及其作用机制。方法: 培养3T3L1细胞,诱导为白色脂肪细胞,运用RT-PCR技术检测组胺和苯海拉明对白色脂肪细胞中脂肪细胞脂肪酸结合蛋白2(aP2 mRNA)水平的影响。结果: 组胺可以增加分化成熟的3T3L1小鼠胚胎成纤维细胞(前脂肪细胞)aP2的表达,剂量效应图显示组胺为 20 μmol/L 时aP2表达量为最高,时间效应图显示在共同孵育 6 h 后aP2表达量达到最高;苯海拉明与同剂量组胺相比,可以显著降低aP2的表达(P<0.05)。结论: 组胺可以刺激分化成熟的3T3L1细胞的aP2的表达,苯海拉明可以抑制组胺的效应。组胺能增加脂肪细胞的脂肪合成,维持白色脂肪细胞能量代谢的平衡。

关键词: 组胺, 脂肪合成, 脂肪酸结合蛋白2

Abstract: AIM: To study the effect of histamine on fat synthesis in the differentiated 3T3L1 cell in vitro and its mechanisms.METHODS: The 3T3L1 cells cultured and induced it to white fat cells.The levels of aP2 mRNA in the adipocytes under the treatment of histamine and diphenhydramine were detected by RT-PCR techniques.RESULTS: Histamine increased the expression of aP2 mRNA in the adipocytes, the concentration-dependent effect curve discovered the highest expression of aP2 mRNA was at 20 μmol/L, the time-dependent effect curve discovered the highest expression of aP2 mRNA was at 6 h.Compare with the same concentration of histamine, diphenhydramine decreased the expression of aP2 mRNA(P<0.05).CONCLUSION: Histamine increased the expression of aP2 mRNA in the differentiated 3T3L1 cell.Diphenhydramine inhibited the expression of aP2 mRNA. Histamine can increase the fat synthesis and maintain the balance of energy metabolism in the white adipocytes.

Key words: Histamine, Fat synthesis, aP2 mRNA

中图分类号:

R965.2

曾慧, 刘昭前. 组胺对白色脂肪细胞脂肪合成以及调控的机制研究[J]. 中国临床药理学与治疗学, 2010, 15(12): 1343-1347.

ZENG Hui, LIU Zhao-qian. Effect of histamine on fat synthesis and regulation in the white adipocytes: a mechanism research[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(12): 1343-1347.

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