槲寄生凝集素腹腔内给药对腹腔巨噬细胞功能的影响

中国临床药理学与治疗学 ›› 2010, Vol. 15 ›› Issue (2): 126-129.

槲寄生凝集素腹腔内给药对腹腔巨噬细胞功能的影响

严梅娣¹, 叶孟², 林蕾², 倪曙民², 王少敏², 刘素荣²

¹宁波市第七医院普外科,宁波 315202,浙江;
²宁波大学医学院附属医院,宁波 315000,浙江

收稿日期:2009-10-09 修回日期:2009-12-23 出版日期:2010-02-26 发布日期:2020-09-18
通讯作者: 叶孟,男,博士,副教授,主要从事肿瘤化疗和肿瘤信号转导途径研究。Tel: 0574-87035270 E-mail: dryemeng@yahoo.com.cn
作者简介:严梅娣,女,主治医师,主要从事普外工作。Tel: 13567430385 E-mail: liangw961@sina.com
基金资助:
浙江省自然基金项目(Y206243);宁波市博士基金项目(2005A610020);宁波市医学科研项目(200530)

Effect of Mistletoe Lectins on immunological roles of peritoneal macrophages by intraperitoneal administration

YAN Mei-di¹, YE Meng², LIN Lei², NI Shu-min², WANG Shao-min², LIU Shu-rong²

¹ Department of Surgery, Ningbo Seventh Hospital, Ningbo 315002,Zhejiang,China;
² The Affiliated Hospital of Medical School of Ningbo University, Ningbo 315000, Zhejiang,China

Received:2009-10-09 Revised:2009-12-23 Online:2010-02-26 Published:2020-09-18

摘要/Abstract

摘要： 目的:探讨小鼠腹腔内注射槲寄生凝集素对腹腔巨噬细胞功能的影响。方法:16只小鼠分为腹腔内给药组和空白组,分别取腹腔内巨噬细胞并计数,采用ELISA法测定TNF-α表达情况,Griess法测定NO表达,MTT法检测腹腔内巨噬细胞对肠癌HCT116细胞的杀伤作用。结果:腹腔内给槲寄生凝集素后可明显增加腹腔内巨噬细胞数,并增加TNF-α和NO的表达水平;激活的巨噬细胞对HCT116细胞的杀伤作用增强,其杀伤作用与TNF-α和NO表达时间一致。结论:腹腔内注射槲寄生凝集素可激活腹腔内巨噬细胞,激活的巨噬细胞对HCT116细胞有杀伤作用。

关键词: 结直肠肿瘤, 槲寄生凝集素, 巨噬细胞, 腹腔内化疗

Abstract: AIM: To investigate the effect of peritoneal macrophages in mice on tumoricidal activity against colon cancer HCT116, following intraperitoneal injection of Mistletoe Lectins. METHODS: 16 mice were divided into control group and intraperitoneal chemotherapy group. The number of peritoneal macrophages was counted by trypan blue exclusion. The amount of TNF-α and NO expressed by peritoneal macrophages was measured by ELISA and Griess, respectively. The cytotoxity of peritoneal macrophages supernatant against HCT116 was evaluated by MTT assay. RESULTS: It was found that the amount of peritoneal macrophages was increased, the expressions of TNF-α and NO were increased and the cytotoxicity against HCT116 in vitro was increased after intraperitoneal injection of Mistletoe Lectins. CONCLUSION: Intraperitoneal injection of Mistletoe Lectins can activate the peritoneal macrophages in mice and enhance the cytotoxity of the supernatant against HCT116 in vitro.

Key words: Colorectal neoplasm, Mistletoe Lectins, Macrophage, Intraperitoneal chemotherapy

中图分类号:

R965.2

严梅娣, 叶孟, 林蕾, 倪曙民, 王少敏, 刘素荣. 槲寄生凝集素腹腔内给药对腹腔巨噬细胞功能的影响[J]. 中国临床药理学与治疗学, 2010, 15(2): 126-129.

YAN Mei-di, YE Meng, LIN Lei, NI Shu-min, WANG Shao-min, LIU Shu-rong. Effect of Mistletoe Lectins on immunological roles of peritoneal macrophages by intraperitoneal administration[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(2): 126-129.

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