三七总苷对高脂血症性骨质疏松大鼠骨代谢的影响

摘要/Abstract

摘要： 目的: 研究三七总苷(PNS) 对大鼠高脂血症性骨质疏松的作用。方法: 取SPF 级3 月龄未交配SD 大鼠30 只随机分为3 组, 每组10 只。正常对照组:普通饲料喂养, 蒸馏水5 mL,kg ^-1,d^-1 灌胃;模型组:脂肪乳剂5 mL,kg^-1,d^-1灌胃;PNS 组:上午予脂肪乳剂5 mL,kg^-1,d^-1 灌胃, 下午予PNS200 mg ·kg ^-1,d^-1 灌胃, 连续20 周。实验结束后用骨组织形态计量学方法, 测定大鼠右侧胫骨近端松质骨静态参数和动态参数, 并观察血清生化指标。结果: 与模型组比较, PNS 组游离脂肪酸(FFA)、TC、低密度脂蛋白胆固醇(LDL-C) 均明显降低(P<0.05), 高密度脂蛋白胆固醇(HDL-C) 明显升高(P<0.05);松质骨形态计量学静态参数骨小梁面积百分数(%T b.Ar)、骨小梁数量(Tb.N)、骨小梁厚度(Tb.Th) 均增加(P<0.01), 骨小梁分离度(Tb.Sp) 明显减小(P<0.01);动态参数荧光周长百分率(%L.Pm)、骨形成率(BFR/BS、BFR/BV) 均明显增加(P<0.05)。结论: 脂肪乳剂长期灌胃可导致大鼠脂质代谢紊乱, 并伴有高脂血症性骨质疏松症。PNS可显著降低血脂并促进骨的形成, 对大鼠高脂血症所致骨质疏松有一定防治作用。

关键词: 三七总苷, 骨质疏松, 高脂血症, 骨组织

Abstract: AIM: To explore the effects of panax notoginseno sides (PNS) on bone histomor phometry in rats with hyperlipidemia-inducedo steoporosis. METHODS: Thirty 3-month-old Sprague-Daw ley rats were randomly divided into three groups with 10 per group as follows. Control group was intrag astrically given distilled water at the do se of 5 mL ·kg^-1,d^-1 for 20 weeks ;Model group was intragast rically given lipid emulsion at the do se of 5 mL, kg ^-1,d^-1 for 20 weeks ;PNS preventing group was intragastrically given lipid emulsion at the dose of 5 mL ·kg^-1 ·d^-1 in the morning and PNS at the do se of 5 mL ·kg^-1 ·d^-1 at afternoon for 20 weeks.At the end of the experiment, the right longitudinal proximal tibial metaphy seal parameters of rats were performed undecalcifiedly and used for bone histomo rphometry analysis. And the blood serum contents of FFA, TG, TC, HDL-C and LDL-C were measured by biochemical analy sis. RESULTS: Compared with model group, the blood serum contents of FFA, TC and LDL-C were decreased and HDL-C was increased significantly (P<0.01), the PTM cancellous bone of %Tb. Ar, Tb. Th and Tb.N were increased (P<0.01) and Tb.Sp was decreased significantly (P<0.01).The dynamic parameters in cancellous bone of %L.Pm and bone format ion rate (BFR/BS, BFR/BV) were increased(P<0.05). CONCLUSION: Long-term gastric perfusion of lipid emulsion could induce hyperlipidemia-induced osteoporosis, and PNS could partly prevent it.

Key words: Panax notog inseno sides, Osteoporosis, Hype rlipidemia, Bone histomorphometry

中图分类号:

R965

纳青青, 谢华. 三七总苷对高脂血症性骨质疏松大鼠骨代谢的影响[J]. 中国临床药理学与治疗学, 2010, 15(3): 277-281.

NA Qing-qing, XIE Hua. Effects of panax notoginsenosides on bone histomor phometry in rats with hyperlipidemia-induced osteoporosis[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(3): 277-281.

参考文献

[1] 尤婷婷, 吴铁, 邹丽宜.高脂血症致大鼠骨质疏松作用初探[J].中国临床药理学与治疗学, 2007, 12(3):299-303.
[2] Parfitt AM, Drezner M K, Glo rieux FH.Bone histomor phomet ry:standa rdiza tion of nomenclature,symbo ls and units.Repo rts of the ASBMR Histomo rphometry Committee[J].J Bone Miner Res1987, 2(6):595-610.
[3] Mundy G, Ga rrett R, H arris S, et al.Stimulationof bo ne fo rmation in vitro and in rodents by stains[J].Science, 1999, 286:1946-1949.
[4] Sugiyama M, Kodama T, Konishi K, et al.Compactin and simva sta tin, but not prav astatin, induce bo ne mo rpho genetic pro tein-2 in human osteo sa rcoma cells[J].Biochem Biphys Res Commun, 2000,271:688-699.
[5] Ohnaka K, Shimoda S, Naw ata H, et al.Pitav astatin enhanced BM P-2 and osteo calcin expression by inhibition of Pho-a ssocia tedkinase in humano steoblasts[J].Bio chem Biophys Re s Commun, 2001,287:337-342.
[6] 殷力, 李月白, 许建中, 等.地塞米松调节骨髓基质细胞成脂及成骨分化的研究[J].中华实验外科杂志,2002, 17(6):557-558.
[7] Do ng TX, Cui XM, Song ZH, et al.Chemical asse ssment of ro ots of Panax notog inseng in China regional and sea so nal variatio ns in it active constituents[J].Ag ric Food Chem, 2003, 51:4617-4623.
[8] 吴丽萍. 三七总苷对成骨细胞增值, 分化及OPG 表达影响的研究[J].中国骨质疏松杂志, 2005, 10(2):239-243.
[9] 刘剑毅, 李世荣.三七总苷对人瘢痕疙瘩成纤维细胞的作用[J].中国临床康复, 2003, 7(32):4314-4315.
[10] Cui L, Wu T, Liu XQ, et al.Preventive effects of ginse no sides on osteopenia of rats induced by o variectomy[J].Acta Pha rmacal Sin, 2001, 22(5):428-434.
[11] Zhang XY.Molecular base of osteoclast bone reso rption mediated by osteoblast[J].Foreign Med Sci Sect Geratol Fase, 2000, 21(4):159-161.
[12] At tele AS, Wu JA, Yuan CS.Ginseng Pha rmacology:multiple constituents and multiple actions[J].Biochemical Pharmacolog y, 1999, 58(11):1685-1693.
[13] Jin JM, Tao Helen, Gao RL, et al.Effects of ginsenosides on pro liferation and diffe rentiatio n of CD(34) + hemo po ietic stemcell[J].Chin J Integ rated Tradit West Med, 2000, 20(9):673-676.
[14] Parfitt AM.Osteo po ro sis:skeleton hetero geneity and the purpo ses of bone remodeling[M] // Marcus R, Fe ldma n D, Kelsey J.Osteo po ro sis.San Diego:Academic Press, 1996:286-329.

[1]	赵璐, 李付勇, 温滨红, 李道伟. 他汀类药物治疗伴高脂血症偏头痛患者的临床疗效分析[J]. 中国临床药理学与治疗学, 2023, 28(6): 671-675.
[2]	赵阳婷, 陈重阳, 潘斌晶, 吕小羽, 刘靖芳. 三甲胺N-氧化物：骨质疏松治疗的潜在靶点[J]. 中国临床药理学与治疗学, 2023, 28(10): 1161-1167.
[3]	叶森, 陈彦震, 赖丽萍, 钟凌剑, 余长章. 超声与X线引导椎体成形术穿刺进针路径的手术疗效及安全性对比研究[J]. 中国临床药理学与治疗学, 2022, 27(5): 551-557.
[4]	杜尘, 田禾, 曹阳, 王拥军, 张岩. 从内分泌激素探讨多囊卵巢综合征诱发骨代谢异常的研究进展[J]. 中国临床药理学与治疗学, 2022, 27(3): 328-333.
[5]	许可, 高骏伟, 刘鹏, 申正日, 贾慧, 吴宸广, 田锋, 王立范, 李平. 糖肾方激活PGC-1α/LXR/ABCA1信号通路改善高脂诱导的巨噬细胞胆固醇摄取及流出[J]. 中国临床药理学与治疗学, 2021, 26(9): 978-985.
[6]	邓祖跃，黄俊俊. 仙灵骨葆联合益生菌对骨质疏松性骨折大鼠的愈合作用[J]. 中国临床药理学与治疗学, 2018, 23(10): 1126-1131.
[7]	喻琴云，苏孝共，包琦瑛，陈晓燕. 白藜芦醇对去卵巢骨质疏松大鼠Wnt/β-Catenin通路的影响[J]. 中国临床药理学与治疗学, 2017, 22(6): 645-649.
[8]	李西，靳高凤，刘志军，李新华，刘名义，张红，熊玉卿. MiR-148a在国人血浆中的分布及与高脂血症的关联性探讨[J]. 中国临床药理学与治疗学, 2017, 22(6): 659-663.
[9]	喻琴云，詹晓峰，黄彦. 刺芒柄花素对去势骨质疏松大鼠P38MAPK/MMP-9信号通路的影响[J]. 中国临床药理学与治疗学, 2017, 22(12): 1371-1376.
[10]	李晓莉，沙南南，陈楠，王拥军，张岩. 烟、酒致骨质疏松症的实验研究进展[J]. 中国临床药理学与治疗学, 2017, 22(11): 1299-1303.
[11]	杨毅，官俏兵，郭丽，李文燕，张慧，韩晨阳. 依折麦布与考来替泊交替给药对于高脂血症大鼠脂代谢的调节作用[J]. 中国临床药理学与治疗学, 2017, 22(10): 1112-1116.
[12]	姜海，姜国志，陈钟，李振江. 姜黄提取物联合阿托伐他汀钙对高脂血症小鼠降血脂作用的研究[J]. 中国临床药理学与治疗学, 2017, 22(1): 48-51.
[13]	钟佳贤，林泽苗，贾欢欢，吴玉娥，陈珺，李青南. 护骨胶囊对去卵巢大鼠腰椎松质骨的影响[J]. 中国临床药理学与治疗学, 2016, 21(9): 1007-1011.
[14]	陈丽思，黄志荣，吴海游，许碧莲，崔燎，吴铁，邹丽宜. 辅酶Q10对去卵巢大鼠骨质和骨密度的影响[J]. 中国临床药理学与治疗学, 2016, 21(8): 868-872.
[15]	贝家欣, 夏海珊, 卢家淇, 许碧莲, 崔燎, 吴铁, 邹丽宜. 人参皂苷Rb1抗骨质疏松作用机制的研究进展[J]. 中国临床药理学与治疗学, 2016, 21(8): 950-955.