BAG-1 及NF-κB P65 在多发性骨髓瘤患者骨髓单个核细胞中的表达

中国临床药理学与治疗学 ›› 2010, Vol. 15 ›› Issue (3): 292-297.

BAG-1 及NF-κB P65 在多发性骨髓瘤患者骨髓单个核细胞中的表达

黄来全, 韦中玲, 张军, 张岱云, 汪兴洪

安徽皖南医学院附属弋矶山医院血液内科, 芜湖241001, 安徽

收稿日期:2009-12-07 修回日期:2009-12-07 发布日期:2020-10-14
作者简介:黄来全, 男, 硕士, 主治医师, 研究方向:恶性血液病发病机制研究。Tel:13675531760 E-mail:huanglaiquan@yahoo.com.cn
基金资助:
安徽省卫生厅自筹项目(2008B045)

Expressions of BAG-1 and NF-κB P65 in the mononuclearcell of multiplemyeloma patients

HUANG Lai-quan, WEI Zhong-lin, ZHANG Jun, ZHANG Dai-yun, WANG Xing-hong

Department of Blood,Yijishan Hospital of Wannan Medical College, Wuhu 241001, Anhui, China

Received:2009-12-07 Revised:2009-12-07 Published:2020-10-14

摘要/Abstract

摘要： 目的: 通过观察核酸水平Bcl-2结合抗凋亡基因1 (Bcl-2-A sso ciated A thano gene 1, BAG-1) 及NF-κB P65 在多发性骨髓瘤(multiple myeloma,MM) 的表达, 探讨BAG-1 及NF-κB P65 在MM 发病机制中的作用及其临床意义, 为MM 的治疗寻找相关的理论依据。方法: 选取皖南医学院附属弋矶山医院2006 年10 月-2008 年10 月初诊MM 患者为实验组, 同时选取骨外伤患者为对照组, 抽取患者骨髓液提取单个核细胞, 用于BAG-1 及NF-κB P65 mRNA RT-PCR 检测。结果: 初诊MM 患者BAG-1 mRNA 在单个核细胞中表达率为71.4%, 高于对照组(P =0.007)。NF-κB P65 mRN A 在初诊骨髓瘤患者单个核细胞中表达率为75.0%, 高于对照组(P =0.004)。BAG-1 mRNA 与NF-κB P65 mRNA 呈正相关。BAG-1 及NF-κB P65 高表达的MM 患者肿瘤负荷高、与多项临床指标有关。结论: BAG-1 及NF-κB P65 在MM 患者单个核细胞中高表达, 与对照组相比差异有统计学意义, 与临床多项指标有关, 经分析可能参与疾病的发生发展, 可作为预后参考指标之一。

关键词: BAG-1, NF-κB P65, 多发性骨髓瘤, RTPCR

Abstract: AIM: To investigate the expressions of BAG-1 and NF-κB P65 in the multiple my eloma (MM) and approach the effects of BAG-1 and NF-κB P65 in the pathogenesi s of MM and their clinical sig nificance, which would be useful tofind a theo retical basis for thet reatment of MM. METHODS: The pat ients diag no sed MM in Yiji shan ho spital of wannan medical college from 2006.10 to 2008.10 as the experimental group, w hi le the bo net rauma patients selected as the co nt ro l gro up.The mononuclearcells of bone marrow in the patient swere ext racted and the BAG-1and NF-κB P65 mRNA RT-PCR were detected. RESULTS: The expression of BAG-1 mRNA (71.4 %) in the MM patients was hig her than that in the control group(P=0.007).The expre ssion of NF-κB P65 mRNA (75.0 %) in the MM patients was hig her than that in the cont rol gro up(P =0.004).There was a positive correlation between the BAG-1 and NF-κB P65 mRNA. The tumo rload was high in the MM patients with overexpression of BAG-1 and NF-κB P65, which was ralated to a number of clinical pa rameters indexes.BAG-1 and NF-κB P65 we re po sitive expressed of MM tumor load, relatived with anumbe r of clinical indicato rs. CONCLUSION: The expressions of BAG-1 and NF-κB P65 in MM are high, there are no dif ference betw een the experimental group and control group.BAG-1and NF-κB P65 were related to a number of clinical indexes.BAG-1 and NF-κB P65 may be involved in the occurrence and the development of disease, and they can be used as one of the prog no sis indexes.

Key words: BAG-1, NF-κB P65, Multiple my eloma, RT-PCR

中图分类号:

R733.3

黄来全, 韦中玲, 张军, 张岱云, 汪兴洪. BAG-1 及NF-κB P65 在多发性骨髓瘤患者骨髓单个核细胞中的表达[J]. 中国临床药理学与治疗学, 2010, 15(3): 292-297.

HUANG Lai-quan, WEI Zhong-lin, ZHANG Jun, ZHANG Dai-yun, WANG Xing-hong. Expressions of BAG-1 and NF-κB P65 in the mononuclearcell of multiplemyeloma patients[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(3): 292-297.

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