银杏叶提取物EGB761对D-半乳糖诱导的心肌细胞老化的保护作用

中国临床药理学与治疗学 ›› 2010, Vol. 15 ›› Issue (4): 376-380.

银杏叶提取物EGB761对D-半乳糖诱导的心肌细胞老化的保护作用

郭长青¹, 郭妍¹, 陈相健², 王德国³, 刘静¹

¹江苏省南京市南京医科大学第一附属医院老年医学科,
²心肌研究所,
³心血管科,南京 210029,江苏

收稿日期:2009-12-10 修回日期:2009-12-26 发布日期:2020-09-17
通讯作者: 郭妍,女,博士,副教授,主任医师,硕士生导师,研究方向:心肌老化与血管硬化。Tel:025-83718836 E-mail:guoyan51@hotmail.com
作者简介:郭长青,女,在读硕士,研究方向:心肌老化与血管硬化。Tel: 025-83718836 E-mail: nihaoguochangqing@163.com
基金资助:
国家自然科学基金项目(30772781)

Evaluation of prevention effects of Extract of Ginkgo Biloba (EGB761) on aging cardiomyocytes cell induced by D-galactose

GUO Chang-qing¹, GUO Yan¹, CHEN Xiang-jian², WANG De-guo³, LIU Jing¹

¹Department of Geronotology,
²Research Institute of Cardiovascular Disease,
³Department of Cardiology,First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu, China

Received:2009-12-10 Revised:2009-12-26 Published:2020-09-17

摘要/Abstract

摘要： 目的: 探讨银杏叶提取物EGB761对D-半乳糖诱导的心肌细胞老化的影响及其可能机制。方法: 用 5 g/L D-半乳糖处理培养心肌细胞建立衰老模型,并以不同浓度EGB761(5、10、20 μg/mL)分别干预 48 h。以β-半乳糖苷酶染色判断细胞衰老,ELISA法检测心肌细胞晚期糖基化终产物(advanced glycation end products,AGEs)的含量。显微镜下观察心肌细胞的形态和搏动情况。结果: 与正常对照组相比,D-半乳糖诱导的心肌细胞内β-半乳糖苷酶染色阳性率显著提高(75.6%±4.9% vs 17.2%±2.9%, P<0.01);细胞内AGEs含量明显增高[(703±32 ) vs (93±26) pg/mL, P<0.01];同时出现细胞形态学和搏动的异常。与D-半乳糖组比较,不同浓度(5、10、20 μg/mL)EGB761干预组β-半乳糖苷酶染色阳性率显著下降(各组分别为 57.7%±7.9%、49.7%±9.2%、34.0%±6.6%, P<0.01),同时AGEs含量显著降低[分别为(404±33)、(357±25)、(249±77) pg/mL,P<0.01],心肌细胞的搏动明显增加。结论: EGB761可通过抑制心肌细胞的非酶糖基化,预防D-半乳糖诱导的心肌细胞老化。

关键词: 银杏叶提取物EGB761, 晚期糖基化终产物, 心肌细胞, 老化, β-半乳糖苷酶

Abstract: AIM: To investigate the preventive effects of Extract of Ginkgo Biloba (EGB761) on the cell aging of cardiomyocytes induced by D-galactose and its latent mechanism. METHODS: Cell aging of cultured neonatal rat cardiomyocytes was created by 5 g/L D-galactose. EGB761 was added to cultured cardiomyocytes at different concentration (5, 10 and 20 μg/mL, respectively) for 48 hours. Cell aging was determined by a senescent β-galactosidase staining. Advanced glycation end products (AGEs) were quantitative analyzed by ELISA. The morphology and beat of cardiac myocytes were observed under an invert microscope. RESULTS: D-galactose resulted in a significantly increased β-galactosidase positive cardiomyocytes compared with the controls (75.6%±4.9% vs 17.15%±2.9%, P<0.01), concomited with elevated AGEs[(703±32)vs (93±26) pg/mL, P<0.01], abnormal cellular morphology and beat rate. EGB761 treated groups showed significant decrease in both β-galactosidase positive cells(57.7%±7.9%, 49.7%±9.2%, 34.0%±6.6%, P<0.01) and AGEs levels [(404±33), (357±25), (249±77) pg/mL, respectively, all P value were less than 0.01], accompanied by relative normal morphology and beat rate. CONCLUSION: EGB761 has anti-aging actions on cardiomyocytes through inhibit non-enzymatic reactions.

Key words: Extract of Ginkgo Biloba, Advanced glycation end products(AGEs), Cardiomyocyte, Aging, β-galactosidase

中图分类号:

R965.2

郭长青, 郭妍, 陈相健, 王德国, 刘静. 银杏叶提取物EGB761对D-半乳糖诱导的心肌细胞老化的保护作用[J]. 中国临床药理学与治疗学, 2010, 15(4): 376-380.

GUO Chang-qing, GUO Yan, CHEN Xiang-jian, WANG De-guo, LIU Jing. Evaluation of prevention effects of Extract of Ginkgo Biloba (EGB761) on aging cardiomyocytes cell induced by D-galactose[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(4): 376-380.

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