黄芪多糖对哮喘小鼠气道重构的干预作用

中国临床药理学与治疗学 ›› 2010, Vol. 15 ›› Issue (4): 385-390.

黄芪多糖对哮喘小鼠气道重构的干预作用

宋泽庆¹, 朱艳芬¹, 姚卫民¹, 王晖²

¹广东医学院附属医院呼吸内科, 湛江 524001,广东;
²广东药学院中药系,广州 510006,广东

收稿日期:2010-03-16 修回日期:2010-04-14 发布日期:2020-09-17
作者简介:宋泽庆,男,硕士,主任医师,硕士生导师,主要从事哮喘的基础和临床研究。Tel: 0759-2387581 E-mail: zeqingsong@yahoo.com
基金资助:
广东省科技计划项目(2006B60101064)

Effects of astragalus polysaccharide on airway remodelling in the asthma modle of mouse

SONG Ze-qing¹, ZHU Yan-fen¹, YAO Wei-min¹, WANG Hui²

¹Department of Respiratory Medicine, Affiliated Hospital, Guangdong Medicine College, Zhanjiang 524001,Guangdong, China;
²School of Traditional Chinese Medicine, Guangdong Pharmaceutical College, Guangzhou 510006, Guangdong, China

Received:2010-03-16 Revised:2010-04-14 Published:2020-09-17

摘要/Abstract

摘要： 目的: 探讨黄芪多糖(astragalus polysaccharide, APS)对哮喘小鼠气道重构的干预作用。方法: 60只4～6周龄SPF(Specific Pathogen Free)级BALB/c小鼠,随机均分为对照组、哮喘组、APS治疗组和地塞米松(dexamethasone, DXM)阳性对照组;用卵蛋白(ovalbumin, OVA)致敏/激发哮喘;APS治疗组和DXM阳性对照组分别用APS和DXM肌肉注射治疗。采用二步法免疫组化技术对小鼠肺组织α平滑肌肌动蛋白(smooth muscle actin-α, α-SMA)表达进行检测, 采用计算机图像分析系统对气道重塑进行分析。结果: 与对照组对比,哮喘组小鼠肺泡灌洗液(bronchoalveolar lavage fliud, BALF)中白细胞计数显著增加(P＜0.01),α-SMA强阳性表达,气道壁厚度(d/Pi)、气道壁面积(WA/Pi)、气道平滑肌面积(Wam/Pi)和平滑肌细胞计数(N/Pi)均明显增加(P<0.05 或P<0.01);与哮喘组对比,APS治疗组和DXM阳性对照组BALF中细胞计数显著减少(P<0.001),α-SMA阳性表达,d/Pi、WA/Pi、Wam/Pi和N/Pi均显著减小(P<0.05或P<0.01)。结论: APS可抑制哮喘小鼠的气道重塑,这种抑制作用可能是通过调节α-SMA的表达来实现的。

关键词: 哮喘, 气道重构, 黄芪多糖, α平滑肌肌动蛋白

Abstract: AIM:To evaluate the effects of astragalus polysaccharide (APS) on the airway remodeling in asthmatic mice. METHODS: Sixty SPF BALB/c mouse were randomly divided into four groups: control group, asthmatic model group, APS treatment group and dexamethasone (DXM) treatment group. The experimental groups were sensitized with ovalbumin (OVA), cured with APS or DXM, then excited with 1%OVA. The method of two-step immunohistochemistry and techniques of computer-assisted image analysis were used to detect the changes of the airway remodelling and the expression of α-SMA. RESULTS: Compared with those in the control group, in asthmatic model group, the counts of inflammation cells were significantly increased (P<0.01); the expression of α-SMA was strongly positive; while the wall thickness(d/Pi) and wall area(WA/Pi) of the bronchi were significantly increased(P<0.05 or P<0.01), and so did the area of smooth muscle(Wam/Pi) and the count of smooth muscle cells(N/Pi)(P<0.05 or P<0.01). Compared with the asthmatic model group, in both APS inhalation group and DXM treatment group, the results of α-SMA showed positive expression, the numbers of inflammation cells were notably decreased (P<0.01), there also were great decreases of d/Pi,WA/Pi,Wam/Pi and N/Pi. CONCLUSION: With the treatment of APS, the course of the airway remodeling can be inhibited. The regulation on the expression level of α-SMA may be one of the mechanisms controlling the airway remodeling.

Key words: Asthma, Airway remodeling, Astragalus polysaccharide, α-SMA

中图分类号:

宋泽庆, 朱艳芬, 姚卫民, 王晖. 黄芪多糖对哮喘小鼠气道重构的干预作用[J]. 中国临床药理学与治疗学, 2010, 15(4): 385-390.

SONG Ze-qing, ZHU Yan-fen, YAO Wei-min, WANG Hui. Effects of astragalus polysaccharide on airway remodelling in the asthma modle of mouse[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(4): 385-390.

参考文献

[1] Camoretti-Mercado B. Targeting the airway smooth muscle for asthma treatment[J]. Transl Res, 2009,154(4):165-174.
[2] Dekkers BG, Maarsingh H, Meurs H, et al. Airway structural components drive airway smooth muscle remodeling in asthma[J]. Proc Am Thorac Soc, 2009,6(8):683-692.
[3] 黄翠萍,杨和平,杨颖乔,等.黄芪注射液对哮喘大鼠p38MAPK和Th1／Th2类细胞因子变化的影响[J]. 中国病理生理杂志, 2008,24(12):2386-2390.
[4] 宋泽庆,林璘,朱艳芬.黄芪多糖对哮喘小鼠特异性免疫治疗的增强作用[J]. 中国免疫杂志, 2010,26(2):132-135.
[5] Bai A, Eidelman DH, Hogg JC, et al. Proposed nomenclature for quantifying subdivisions of the bronchial wall[J]. J Appl Physiol, 1994,77(2):1011-1014.
[6] An SS,Fredberg J. Biophysical basis for airway hyperresponsiveness[J]. Can J Physiol Pharmacol, 2007,85(7):700-714.
[7] Bentley JK, Hershenson MB.Airway smooth musele growth in asthma: proliferation,hypertrophy,and migration[J]. Proe Am Thorae Soe, 2008,5(1):89-96.
[8] Freyer AM, Johnson SR,Hall IP. Effeets of growth factors and extra cellular matrixon survival of human airway smooth muscle cells[J]. Am J Res Pir Cell Mol Biol, 2001,25(5):569-576.
[9] Kazi AS,Lotfi S,Goncharova EA,et al.Vascular endothelial growth factor-induced secretion of fibronectin15 ERK dependent[J]. Am J Physicl Lung Cell Mol Physiol, 2004,286(3):539-545.
[10] Siddiqui S, Martin JG.Structural aspects of airway remodeling in asthma[J]. Curr Allergy Asthma Rep, 2008,8(6):540-547.
[11] Bush A.How early do airway in flanimation and remodeling occur[J].Allergol Int, 2008,57(1):11-19.
[12] 江茵,黎东明,陈敏.黄芪多糖对哮喘大鼠气道炎症及IL-3表达的影响[J].中国现代医学杂志, 2009,19(12):1779-1781.
[13] 叶辉,李昌崇,陈利中,等.布地奈德对哮喘大鼠碱性成纤维细胞生长因子蛋白和mRNA表达的影响[J]. 中国临床药理学与治疗学, 2006,11(7):739-742.

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