不同基源川贝母镇咳、祛痰功效差异性实验研究

中国临床药理学与治疗学 ›› 2010, Vol. 15 ›› Issue (6): 612-616.

不同基源川贝母镇咳、祛痰功效差异性实验研究

周宜¹, 丁红², 阎博华², 李继书¹, 刘松山², 李明权², 王帅¹, 丰芬², 黄晓燕³

¹成都中医药大学,成都 610075,四川;
²四川省中医医院,成都 610072,四川;
³成都康弘制药有限公司,成都 610041,四川

收稿日期:2010-03-18 修回日期:2010-04-06 出版日期:2010-06-26 发布日期:2020-09-16
通讯作者: 周宜,男,博士,副研究员,研究方向:中医药基础理论。 Tel: 13880001154 E-mail: 6524118@qq.com
基金资助:
国家“十一五”科技支撑计划(2007BAI40B06)

Research on the functional difference among different species of Chuan Fritillaria in relieving cough and resolving phlegm

ZHOU Yi¹, DING Hong², YAN Bo-hua², LI Ji-shu¹, LIU Song-shan², LI Ming-quan², WANG Shuai¹, FENG Fen², HUANG Xiao-yan³

¹ Chengdu University of Traditional Chinese Medicine,Chengdu 610075, Sichuan,China;
² Sichuan Province Hospital of Traditional Chinese Medicine, Chengdu 610072, Sichuan,China;
³ Chengdu Kanghong Pharmaceutical Co. Ltd., Chengdu 610041, Sichuan,China

Received:2010-03-18 Revised:2010-04-06 Online:2010-06-26 Published:2020-09-16

摘要/Abstract

摘要： 目的: 观察4种不同基源川贝母镇咳、祛痰的功效差异。方法: (1)将健康豚鼠70只,完全随机分为空白对照组、模型组、阳性对照组、暗紫贝母组、梭砂贝母组、卷叶贝母组和甘肃贝母组7组,连续给药 3 d,末次给药 30 min 后,雾化吸入枸橼酸 60 s。记录咳嗽的潜伏期、5 min 内咳嗽的次数。(2)将健康小鼠60只,随机分为空白对照组、阳性对照组、暗紫贝母组、梭砂贝母组、卷叶贝母组和甘肃贝母组6组,连续给药 3 d,末次给药 30 min 后,由腹腔注射 0.5%酚红溶液 0.5 mL/只。30 min 后,处死小鼠,取出气管,冲洗,再加入5%碳酸氢钠溶液 0.1 mL,离心,取上清液,用酶标仪(波长 570 nm)测定OD值、祛痰指数。结果: (1)与卷叶贝母比较,暗紫贝母组、梭砂贝母组均能延长咳嗽潜伏期(P<0.05)。(2)与梭砂贝母组、甘肃贝母组、卷叶贝母组分别比较,暗紫贝母组能促进酚红排泌OD(optical density)值增加(P<0.05,P<0.01)。结论: 4种不同基源川贝母在镇咳、祛痰功效方面可能存在一定的差异,在镇咳方面暗紫贝母、梭砂贝母优于卷叶贝母、甘肃贝母,在祛痰方面暗紫贝母优于甘肃贝母、卷叶贝母、梭砂贝母。

关键词: 基源, 暗紫贝母, 梭砂贝母, 卷叶贝母, 甘肃贝母, 镇咳, 祛痰

Abstract: AIM: To study the effect of relieving cough and resolving phlegm among different species of Chuan Fritillaria. METHODS: (1)70 Healthy guinea pigs had been randomly divided to 7 groups: the Control group, the coughing model group, the Positive control group, the Fritillaria unibracteata Hsiao et K.C.Hsia group, the Fritillaria delavayi Ftanch group, the Fritillaria cirrhosa D.Don group and the Fritillaria przewalskii Maxim group. Continuously gave them herbs for 3 days. 60 s Citrate had been inhalated about 30 min after the last time of delivery. The Latency of cough and frequency of cough within 5 min were recorded.(2)60 Healthy mice had been randomly divided to 6 groups: the control group, the positive control group, the Fritillaria unibracteata Hsiao et K.C.Hsia group, the Fritillaria delavayi Ftanch group, the Fritillaria cirrhosa D.Don group and the Fritillaria przewalskii Maxim group. Continuously gave them herbs for 3 days. 30 min after the last time of delivery, 0.5 mL of 0.5% Phenol red solution had been intraperitoneally injected for each mouse. The mice were killed after 30 min. Mice's trachea had been separated and douched; 0.1 mL of 5% NaHCO₃ was added. After centrifuge manipulation, the supernatant had been divided. Enzyme labeling had been applied to measure the OD and eliminating phlegm index. RESULTS: (1) Compared with Fritillaria cirrhosa D. Don group, Fritillaria unibracteata Hsiao et K.C.Hsia group and the Fritillaria cirrhosa D.Don group could both prolong the latency of cough (P<0.05). (2) Compared with Fritillaria delavayi Ftanch group, Fritillaria cirrhosa D. Don group, Fritillaria przewalskii Maxim group and Fritillaria unibracteata Hsiao et K.C.Hsia group could increase the OD value of Phenol red excretion for mice's trachea(P<0.05, P<0.01). CONCLUSION: The Fritillaria unibracteata Hsiao et K.C.Hsia may have a better effect of resolving phlegm compared with Fritillaria delavayi Ftanch, Fritillaria cirrhosa D.Don and Fritillaria przewalskii Maxim. The Fritillaria unibracteata Hsiao et K.C.Hsia and Fritillaria delavayi Ftanch may have a better effect of relieving cough compared with Fritillaria cirrhosa D.Don and Fritillaria przewalskii Maxim.

Key words: Species, Fritillaria unibracteata Hsiao et K.C.Hsia, Fritillaria delavayi Ftanch, Fritillaria cirrhosa D. Don, Fritillaria przewalskii Maxim, Relieving cough, Resolving phlegm

中图分类号:

R965.2

周宜, 丁红, 阎博华, 李继书, 刘松山, 李明权, 王帅, 丰芬, 黄晓燕. 不同基源川贝母镇咳、祛痰功效差异性实验研究[J]. 中国临床药理学与治疗学, 2010, 15(6): 612-616.

ZHOU Yi, DING Hong, YAN Bo-hua, LI Ji-shu, LIU Song-shan, LI Ming-quan, WANG Shuai, FENG Fen, HUANG Xiao-yan. Research on the functional difference among different species of Chuan Fritillaria in relieving cough and resolving phlegm[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(6): 612-616.

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