西红花酸对3T3-L1前脂细胞增殖和分化的影响

中国临床药理学与治疗学 ›› 2010, Vol. 15 ›› Issue (7): 753-757.

西红花酸对3T3-L1前脂细胞增殖和分化的影响

张晓明, 钱之玉, 季晖, 杨丽娜, 杨如会, 宋磊

中国药科大学药理教研室,南京 210009,江苏

收稿日期:2010-03-08 修回日期:2010-04-20 出版日期:2010-07-26 发布日期:2020-09-15
通讯作者: 季晖,女,教授,博士生导师,主要从事毒理药理学研究。Tel: 025-86205849 E-mail:huijicpu@163.com
作者简介:张晓明,女,硕士研究生,研究方向:生化药理。E-mail:asby444@yahoo.com.cn;钱之玉,男,教授,博士生导师,主要从事生化药理学研究。Tel: 025-83321139 E-mail: qianzhiyu@163.com

Effects of crocetin on the proliferation and differentiation of 3T3-L1 preadipocyte

ZHANG Xiao-ming, QIAN Zhi-yu, JI Hui, YANG Li-na, YANG Ru-hui, SONG Lei

Department of Pharmacology,China Pharmaceutical University, Nanjing 210009, Jiangsu, China

Received:2010-03-08 Revised:2010-04-20 Online:2010-07-26 Published:2020-09-15

摘要/Abstract

摘要： 目的: 观察西红花酸对3T3-L1前脂细胞增殖分化的影响,并探讨其影响3T3-L1细胞分化的可能作用机制。方法: 培养前脂肪细胞3T3-L1,四甲基偶氮唑盐(MTT)法检测西红花酸对其生长活性和增殖的影响;油红O染色和染色比色法分析脂肪细胞的分化程度;逆转录多聚酶联反应(RT-PCR)检测腺苷酸激活蛋白激酶(AMP-activated protein kinase, AMPK)mRNA的表达;Western blot法检测p-AMPK蛋白表达水平。结果: 西红花酸高、中剂量(10^-5、10^-6 mol/L)组均可以显著抑制前脂细胞的增殖和分化(P＜0.01),而低剂量组(10^-7 mol/L)也可以抑制其增殖、分化(P＜0.05);各剂量的西红花酸都能够增加AMPK的mRNA的表达、提高p-AMPK蛋白表达水平(P＜0.05)。结论: 西红花酸具有抑制前脂细胞增殖分化的作用,可能和增强AMPK的表达有关。

关键词: 西红花酸, 3T3-L1细胞, 增殖分化, 腺苷酸激活蛋白激酶

Abstract: AIM: To observe the effect of crocetin on proliferation and differentiation of 3T3-L1 preadipocyte, and to investigate the possible mechanism involved. METHODS: The 3T3-L1 cells were pretreated with crocetin, while, the MTT method was used to detect the proliferation of the cells, oil red O staining method and spectrophotography were applied to analyze the degree of differentiation. The expression of AMP-activated protein kinase(AMPK)mRNA, which was called as the energy regulator of cell, was detected by reverse transcription PCR (RT-PCR), and the protein level of p-AMPK was determined by western blot. RESULTS: High and middle dose crocetin(10^-5, 10^-6 mol/L) could significantly inhibit the proliferation and differentiation of 3T3-L1 cell(P＜0.01); also, the low dose croctin(10^-7 mol/L) could inhibit its proliferation and differentiation(P＜0.05); croctin could incease the expression of AMPK mRNA and the protein level of p-AMPK(P＜0.05). CONCLUSION: Crocetin inhibit the proliferation and differentiation of 3T3-L1 cell possibly by influence AMPK.

Key words: Crocetin, 3T3-L1 cell, Proliferation and differentiation, AMP-activated protein kinase

中图分类号:

R965.2

张晓明, 钱之玉, 季晖, 杨丽娜, 杨如会, 宋磊. 西红花酸对3T3-L1前脂细胞增殖和分化的影响[J]. 中国临床药理学与治疗学, 2010, 15(7): 753-757.

ZHANG Xiao-ming, QIAN Zhi-yu, JI Hui, YANG Li-na, YANG Ru-hui, SONG Lei. Effects of crocetin on the proliferation and differentiation of 3T3-L1 preadipocyte[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(7): 753-757.

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