葛根素对兔急性肺栓塞溶栓治疗后单核细胞趋化蛋白-1、肿瘤坏死因子-α含量及肺超微结构的影响

中国临床药理学与治疗学 ›› 2010, Vol. 15 ›› Issue (7): 781-785.

葛根素对兔急性肺栓塞溶栓治疗后单核细胞趋化蛋白-1、肿瘤坏死因子-α含量及肺超微结构的影响

姜琴华¹, 陈彦凡², 许先荣¹, 许雯¹, 李国平¹, 王良兴², 陈少贤²

¹浙江省立同德医院,杭州 310012,浙江;
²温州医学院附属第一医院,温州 325000,浙江

收稿日期:2010-05-04 修回日期:2010-06-25 出版日期:2010-07-26 发布日期:2020-09-15
通讯作者: 陈少贤,男,教授,硕士研究生导师,研究方向:中西医结合防治呼吸系统疾病。Tel: 0577-88069354 E-mail: wzchsx@126.com
作者简介:姜琴华,女,硕士,主治医师,研究方向:中西医结合防治血栓栓塞性疾病。Tel: 13588048801 E-mail: qhua01@163.com
基金资助:
浙江省中医药科技计划资助项目(2004C125)

Effect of puerarin on contents of MCP-1, TNF-ɑ and pulmonary ultrastructure after thrombolytic therapy in rabbits with acute pulmonary embolism

JIANG Qin-hua¹, CHEN Yan-fan², XU Xian-rong¹, XU Wen¹, LI Guo-ping¹, WANG Liang-xing², CHEN Shao-xian²

¹Tongde Hospital of Zhejiang Province, Hangzhou 310012, Zhejiang,China;
²The First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, Zhejiang, China

Received:2010-05-04 Revised:2010-06-25 Online:2010-07-26 Published:2020-09-15

摘要/Abstract

摘要： 目的: 探讨葛根素对急性肺血栓栓塞溶栓治疗后单核细胞趋化蛋白-1(MCP-1)、肿瘤坏死因子-α(TNF-ɑ)含量及肺超微结构的影响。方法: 24只日本大耳白兔,随机分为假手术组、单纯溶栓组、葛根素组,经右心导管注入条柱状自体血栓,制备急性肺血栓栓塞实验模型,心导管法监测血流动力学。溶栓后4 h取肺组织,测定肺组织湿干重比(W/D),分别用ELISA及比色法检测肺组织MCP-1、TNF-ɑ含量及髓过氧化物酶(MPO)活性的变化,光镜、电镜下观察肺组织显微超微结构改变。结果: 在溶栓开始后1 h,单纯溶栓组和葛根素组肺动脉平均压(mPAP)即显著下降(P<0.01),且葛根素组mPAP明显低于单纯溶栓组(P<0.05);单纯溶栓组和葛根素组MCP-1、TNF-ɑ及W/D、MPO均明显高于假手术组(P<0.05或P<0.01),葛根素组MCP-1、TNF-ɑ及W/D、MPO均明显低于单纯溶栓组(P<0.05或P<0.01);组织病理及超微结构研究显示,葛根素组血管内皮细胞和肺泡上皮细胞损伤较单纯溶栓组明显减轻。结论: 葛根素可降低急性肺血栓栓塞溶栓后肺组织MCP-1、TNF-ɑ含量,抑制再灌注损伤炎症反应,对溶栓治疗后肺组织有保护作用。

关键词: 葛根素, 肺血栓栓塞, 溶栓治疗, 单核细胞趋化蛋白-1, 肿瘤坏死因子-α

Abstract: AIM: To observe the effect of puerarin on the contents of MCP-1,TNF-ɑ and pulmonary ultrastructure after thrombolytic therapy in acute pulmonary thromboembolism.METHODS: Twenty-four Japanese rabbits were randomly divided into sham operation group, thrombolysis-only group, and puerarin group.Acute pulmonary thromboembolism models of rabbits were established with injection of autologous blood clots through the right heart catheters. Haemodynamic monitoring was performed by introducing heart catheter through right jugular vein. These rabbits were sacrificed at 4 h after thrombolysis, the lung was removed and the ratio of wet to dry of lung tissue weight (W/D) was detected. The contents of MCP-l and TNF-α were also investigated with ELISA. The myeloperoxidase (MPO) activities of lung tissue were detected with colorimetry. The ultrastructure of lung tissue was observed by electronic microscope.RESULTS: The pulmonary arterial mean pressure (mPAP) was decreased immediately at 1 h after thrombolysis both in thrombolysis-only group and puerarin group (P<0.01), the mPAP in puerarin group were significantly lower than those in thrombolysis-only group at 1 h after thrombolysis(P<0.05); The contents of MCP-l and TNF-α, the value of W/D and the MPO activity of lung tissue were significantly increased in thrombolysis-only group and puerarin group than those of sham operation group; The histopathologic analysis showed the injuries of vascular endothelial cells and alveolar cells in thrombolysis-only group were more severe than those in puerarin group.CONCLUSION: Chinese herb puerarin can protect lung against reperfusion injury after thrompolytic therapy in acute pulmonary thrombembolism which may associate with the action of inhibiting inflammation reaction by reducing the contents of MCP-1 and TNF-α.

Key words: Puerarin, pulmonary thromboembolism, thrombolytic therapy, MCP-1, TNF-α

中图分类号:

R965.2

姜琴华, 陈彦凡, 许先荣, 许雯, 李国平, 王良兴, 陈少贤. 葛根素对兔急性肺栓塞溶栓治疗后单核细胞趋化蛋白-1、肿瘤坏死因子-α含量及肺超微结构的影响[J]. 中国临床药理学与治疗学, 2010, 15(7): 781-785.

JIANG Qin-hua, CHEN Yan-fan, XU Xian-rong, XU Wen, LI Guo-ping, WANG Liang-xing, CHEN Shao-xian. Effect of puerarin on contents of MCP-1, TNF-ɑ and pulmonary ultrastructure after thrombolytic therapy in rabbits with acute pulmonary embolism[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(7): 781-785.

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