马钱子抑制兔脊髓损伤的细胞凋亡作用

中国临床药理学与治疗学 ›› 2010, Vol. 15 ›› Issue (8): 880-885.

马钱子抑制兔脊髓损伤的细胞凋亡作用

周红英, 侯群, 戚观树, 裘昌林

浙江中医药大学第一临床医学院,杭州 310006,浙江

收稿日期:2010-04-27 修回日期:2010-06-10 出版日期:2010-08-26 发布日期:2020-09-17
通讯作者: 侯群,男,教授、主任医师,硕士生导师,研究方向:神经系统疾病。E-mail:houqun168@163.com
作者简介:周红英,女,硕士研究生在读,研究方向:神经系统疾病的实验和临床研究。E-mail:zhy8305@126.com
基金资助:
浙江省中医管理局资助项目(2008CA012)

Nux vomica inhibiting apoptosis of spinal cord injury rabbit

ZHOU Hong-ying, HOU Qun, QI Guan-shu, QIU Chang-lin

First Clinical Medical College of Zhejiang Chinese Medical University,Hangzhou 310006,Zhejiang,China

Received:2010-04-27 Revised:2010-06-10 Online:2010-08-26 Published:2020-09-17

摘要/Abstract

摘要： 目的: 探讨马钱子是否通过抑制细胞凋亡来治疗脊髓损伤。方法: 采用改良的Allen's法建立兔脊髓损伤模型。设立假脊髓损伤组、对照组、炙马钱子 40 mg 组(M40组)及炙马钱子 80 mg 组(M80组),分别在给药后 1 d、7 d 及 14 d 用Griess反应检测脊髓标本中的NO含量, 用蛋白免疫印迹法(Western blot)检测c-fos蛋白、Bax蛋白和Bcl-2蛋白的表达。结果: 与对照组相比,M40组及M80组治疗第7天及14天,可不同程度地抑制损伤脊髓中NO、c-fos蛋白、Bax蛋白的表达,上调Bcl-2蛋白的表达。其中,M40组和M80组分别治疗第7天的NO含量与对照组有统计学差异(P＜0.05);M80组治疗第7天时c-fos蛋白与对照组有统计学差异(P＜0.05),治疗 14 d 时有重要统计学差异(P＜0.01);M80组治疗第7天时Bax蛋白、Bcl-2蛋白及两者的比值与对照组间有统计学差异(P＜0.05),M80组治疗 14 d 后Bax蛋白与对照组有统计学差异(P＜0.05)。 结论: 马钱子通过减少损伤脊髓局部NO、c-fos蛋白的表达、下调Bax/Bcl-2比值从而抑制细胞凋亡,阻止脊髓继发性损伤,促进脊髓损伤后神经功能的恢复。

关键词: 马钱子, 脊髓损伤, 凋亡

Abstract: AIM: To explore whether nux vomica treats spinal cord injury by inhibiting apoptosis.METHODS: The animal model of SCI was made in rabbits by modified Allen's method. 60 rabbits were randomly divided into 4 groups: the sham group, control group, 40 mg nux vomica group (group M40) and 80 mg nux vomica group (group M80). After treating for 1, 7 and 14 days , the NO level in the spinal cord was detected with the Griess reaction and the expressions of c-fos protein, tested Bax and Bcl-2 protein were examined by Western blot method (Western blotting).RESULTS: Compared with the control group, the expressions of NO, c-fos protein, Bax protein were decreased, while the expression of Bcl-2 protein was increased after treating for 7 days and 14 days in the group M40 and group M80. After 7 days' treatment, the NO contents in the group M40 and group M80 were lower than the control group (P＜0.05). In the group M80, the expressions of c-fos protein were lower than the control group after 7 days' treatment (P＜0.05) and 14 days' treatment (P＜0.01); the expressions of Bax protein were lower than the control group after 7 days' treatment (P＜0.05) and 14 days' treatment (P＜0.05); the expression of Bcl-2 protein was higher than the control group after 7 days' treatment (P＜0.05); the ratio of Bax/Bcl-2 was lower than the control group after 7 days' treatment (P＜0.05).CONCLUSION: Nux vomica can hold back the secondary spinal cord injury and accelerate the spinal nerve function by reducing the expressions of NO and c-fos protein, and cutting the ratio of Bax/Bcl-2 to suppresse the cell apoptosis.

Key words: Nux vomica, Spinal cord injury, Apoptosis

中图分类号:

R965.2

周红英, 侯群, 戚观树, 裘昌林. 马钱子抑制兔脊髓损伤的细胞凋亡作用[J]. 中国临床药理学与治疗学, 2010, 15(8): 880-885.

ZHOU Hong-ying, HOU Qun, QI Guan-shu, QIU Chang-lin. Nux vomica inhibiting apoptosis of spinal cord injury rabbit[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(8): 880-885.

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