HPLC-FLU法测定人血清中硫酸吲哚酚浓度及其在血液透析病人中的应用

中国临床药理学与治疗学 ›› 2010, Vol. 15 ›› Issue (8): 906-910.

HPLC-FLU法测定人血清中硫酸吲哚酚浓度及其在血液透析病人中的应用

吴淳¹, 石成钢², 李梅², 潘碧妍¹, 邢小敏¹, 冯志强¹, 谢智勇¹

¹中山大学药学院,广州 510006,广东;
²中山大学附属第三医院,广州 510630,广东

收稿日期:2010-06-30 修回日期:2010-08-16 出版日期:2010-08-26 发布日期:2020-09-17
通讯作者: 谢智勇,男,副教授,硕士生导师,研究方向:药物代谢与药代动力学。Tel: 020-39943047 E-mail: xiezy2074@yahoo.com
作者简介:吴淳,女,硕士研究生,研究方向:体内药物分析。Tel: 020-39943047 E-mail: wuchunchzx@163.com
基金资助:
“重大新药创制”国家科技重大专项(2009ZX09304-003,2009ZX09501-017);广东省自然科学基金(8151008901000056)

Determination of indoxyl sulfate in human serum by HPLC-FLU and Its application in hemodialysis patients

WU Chun¹, SHI Cheng-gang², LI Mei², PAN Bi-yan¹, XING Xiao-min¹, FENG Zhi-qiang¹, XIE Zhi-yong¹

¹ School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510006, Guangdong, China;
²The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510630, Guangdong, China

Received:2010-06-30 Revised:2010-08-16 Online:2010-08-26 Published:2020-09-17

摘要/Abstract

摘要： 目的: 建立测定人血清中硫酸吲哚酚浓度的高效液相色谱-荧光检测法,了解血液透析和血液透析滤过对硫酸吲哚酚的清除效果。方法: 血清样品经乙腈沉淀蛋白;采用Hypersil BDS C₁₈ 色谱柱(250 mm×4.6 mm,5 μm);流动相为乙腈-水-三氟乙酸(15∶85∶0.2,V/V/V),流速为 1 mL/min;入射波长为 280 nm,发射波长为 390 nm。分别测定尿毒症患者透析前后血清中硫酸吲哚酚浓度。结果: 线性范围为 0.5~80.0 μg/mL, 线性方程为Y =2.438×10⁴X+6.964×10³,r=0.9986;日内、日间精密度均小于15%,方法回收率为 92.8%~111.4%,提取回收率为 90.9%。血液透析和血液透析滤过后患者血清中硫酸吲哚酚的下降率分别为(19.6±7.3)%和(25.3±7.0)%。结论: 本法简便、快速、灵敏、准确,可用于测定人血清中硫酸吲哚酚浓度。

关键词: 硫酸吲哚酚, 高效液相色谱, 荧光检测, 血液透析

Abstract: AIM: To establish a high-performance liquid chromatography method with fluorescence detector for the determination of indoxyl sulfate (IS) in human serum, and apply to study the clearance effect of IS by hemodialysis and hemodiafiltration.METHODS: The serum was deproteinated by acetonitrile. The chromatographic separation was achieved on a Hypersil BDS C₁₈ column (250 mm×4.6 mm, 5 μm) with the mobile phase consisted of acetonitrile-water-trifluoroacetic (15∶85∶0.2, V/V/V) at a flow rate of 1 mL/min. The fluorescence detector was operated at an excitation and emission wavelengths 280 nm and 390 nm, respectively. The serum level of IS was deteated before and after dialysis in uremic patients.RESULTS: A good linearity was obtained from 0.5 to 80.0 μg/mL with r=0.9986, the linear regression equation was Y=2.438×10⁴X+6.964×10³. The intra-day RSD and inter-day RSD were both less than 15%. The method recoveries were 92.8%-110.4% and the extraction recovery was 90.9%. The reduction ratios of IS after hemodialysis and hemodiafiltration were (19.6±7.3)% and (25.3±7.0)%, respectively. CONCLUSION: The method is simple, sensitive and accurate for the determination of indoxyl sulfate in serum.

Key words: Indoxyl sulfate, HPLC, Fluorescence detection, Hemodialysis

中图分类号:

R969. 1

吴淳, 石成钢, 李梅, 潘碧妍, 邢小敏, 冯志强, 谢智勇. HPLC-FLU法测定人血清中硫酸吲哚酚浓度及其在血液透析病人中的应用[J]. 中国临床药理学与治疗学, 2010, 15(8): 906-910.

WU Chun, SHI Cheng-gang, LI Mei, PAN Bi-yan, XING Xiao-min, FENG Zhi-qiang, XIE Zhi-yong. Determination of indoxyl sulfate in human serum by HPLC-FLU and Its application in hemodialysis patients[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(8): 906-910.

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