拉莫三嗪对大鼠脑缺血再灌注损伤的神经保护作用

中国临床药理学与治疗学 ›› 2011, Vol. 16 ›› Issue (10): 1105-1109.

拉莫三嗪对大鼠脑缺血再灌注损伤的神经保护作用

毛捷, 江晓春, 徐善水, 王宣之

皖南医学院弋矶山医院神经外科,芜湖 241001,安徽

收稿日期:2011-08-12 修回日期:2011-09-28 出版日期:2011-10-26 发布日期:2011-11-02
作者简介:毛捷,男,博士,副主任医师,研究方向:脑保护。Tel: 0553-5739952, E-mail: myw921@yahoo.com

The neuroprojective effects of lamotrigine on rat global cerebral ischemic reperfusion damage

MAO Jie, JIANG Xiao-chun, XU Shan-shui, WANG Xuan-zhi

Department of Neurosurgery, Yijishan Hospital, Wannan Medical College, Wuhu 241001, Anhui, China

Received:2011-08-12 Revised:2011-09-28 Online:2011-10-26 Published:2011-11-02

摘要/Abstract

摘要： 目的: 探讨拉莫三嗪(lamotrigine, LTG)对脑缺血再灌注损伤的神经细胞的保护作用。方法: Pulsinelli四血管闭塞法建立大鼠全脑缺血再灌注损伤模型,观测LTG对脑缺血再灌注损伤后脑细胞外液谷氨酸含量的动态变化,脑组织的病理改变和神经细胞凋亡。结果: (1)再灌注组、治疗组谷氨酸基础水平与对照组基本相同,缺血后迅速升高,再灌注 30 min 达高峰,其后逐渐下降。再灌注组在30~330 min 内显著高于对照组(P<0.05),治疗组在30~330 min 内显著高于对照组,但低于再灌注组(P<0.05)。(2)TUNEL 法发现治疗组凋亡细胞较再灌注组明显减少(P<0.05)。结论: 脑缺血再灌注损伤后,早期使用LTG治疗,明显减轻脑组织中兴奋性氨基酸含量,增加神经元细胞的存活率。

关键词: 拉莫三嗪, 谷氨酸, 体内微透析, 凋亡

Abstract: AIM: To investigate the neuroprojective effects of lamotrigine on rat global cerebral ischemic reperfusion damage. METHODS: Pulsinelli's method was employed to establish cerebral ischemia reperfusion animal model. The dynamic varieties of Glu, patho-change of brain tissue and neurocyte apoptosis were observed after giving lamotrigine. RESULTS: (1) Basic levels of Glu in acute ischemia and reperfusion group and LTG group were same as sham group's. They improved swiftly after lack blood, and reached peak after the brain reperfusion 30 min, then gradually fell. The levels of acute ischemia and reperfusion group were notability higher than sham group's in 30-330 min (P<0.05). The levels of LTG group were notability higher than sham group's, and lower than acute ischemia and reperfusion group's in 30-330 min (P<0.05). (2) By the method of TdT-mediated Dutp-biotin nick end-labeling, positive apoptosis cell in LTG group were decreased remarkably than in acute ischemia and reperfusion group (P<0.05). CONCLUSION: After cerebral ischemia and reperfusion, lamotrigine can reduce the amount of excitatory amino acids, and enhance nerve cell's living rate, and provide obvious protective effects .

Key words: Lamotrigine, Glutamate, Microdialysis, Apoptosis

中图分类号:

R965.2

毛捷, 江晓春, 徐善水, 王宣之. 拉莫三嗪对大鼠脑缺血再灌注损伤的神经保护作用[J]. 中国临床药理学与治疗学, 2011, 16(10): 1105-1109.

MAO Jie, JIANG Xiao-chun, XU Shan-shui, WANG Xuan-zhi. The neuroprojective effects of lamotrigine on rat global cerebral ischemic reperfusion damage[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2011, 16(10): 1105-1109.

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