阵发性房颤诱发大鼠心房肌电重构特征研究

中国临床药理学与治疗学 ›› 2011, Vol. 16 ›› Issue (11): 1205-1209.

阵发性房颤诱发大鼠心房肌电重构特征研究

汤依群, 孙书娟, 尹月淼, 汪旻晖, 黄潞, 单娇娇

中国药科大学临床药学教研室,南京 210009,江苏

收稿日期:2011-08-01 修回日期:2011-10-31 出版日期:2011-11-26 发布日期:2011-11-29
作者简介:汤依群,女,博士,副教授,硕士生导师,研究方向:心血管药理学。Tel: 025-83271070 E-mail: tyq@cpu.edu.cn
基金资助:
国家新药创制重大专项(2009ZX09103-088);江苏省自然科学基金(BK2010434)

Characteristics of atrial electrical remodeling in rats induced by paroxysmal atrial fibrillation

TANG Yi-qun, SUN Shu-juan, YIN Yue-miao, WANG Min-hui, HUANG Lu, SHAN Jiao-jiao

Department of Clinical Pharmacy, China Pharmaceutical University, Nanjing 210009, Jiangsu, China

Received:2011-08-01 Revised:2011-10-31 Online:2011-11-26 Published:2011-11-29

摘要/Abstract

摘要： 目的: 探讨氯化钙-乙酰胆碱混合液引起的房颤大鼠心房电重构特征。方法: 雄性SD大鼠,随机分为正常组和模型组,每组20只。CaCl₂ 10 mg/mL+ACh 66 μg/mL 混合液尾静脉注射诱发大鼠房颤,连续诱发 7 d,正常组静脉注射生理盐水 7 d。心电图监测大鼠房颤持续时间、心房复极相关心电图参数变化。7 d 后,观察正常和房颤大鼠心房肌有效不应期,Western-blot法测定心房肌通道蛋白Kv1.5、Kv4.2/4.3,钙稳蛋白FKBP12.6 和缝隙连接蛋白Cx40的表达。结果: Ach-CaCl₂连续静脉注射引起大鼠房颤持续时间逐渐延长,与正常组相比,房颤大鼠P波变宽,Pd增大,心房有效不应期缩短(P<0.01),同时心房肌Kv1.5、Kv4.2/4.3表达下降,FKBP12.6 减少,Cx40含量下降(P<0.05)。结论: 氯化钙-乙酰胆碱混合液持续给予7 d,大鼠心房发生电重构,特征符合或接近临床房颤基质电重构特征。

关键词: 心房颤动, 电重构, 缝隙连接蛋白40

Abstract: AIM: To investigate the characterisics of atrial electrical remodeling induced by atrial fibrillation(AF) at molecular level.METHODS: Male SD rats were randomized into 2 equal groups. Rats of control group were administrated physiological saline (i.v.) for 7 days. The others were injected ACh-CaCl₂ (i.v.) for 7 days. AF duration and ECG parameters were measured. On day 8, all rats were anesthetized and the atriums were immediately excised for measuring AERP, the expression of Kv 1.5, Kv4.2/4.3, FKBP12.6 and Cx40 protein were detected using western-blot.RESULTS: In model rats, AF duration continually increased from day 1 to day 7 with ACh-CaCl₂ administration. The AERP of model group was shorter than that of control group [(65.3±3.6) ms vs (82.3±0.6) ms; P<0.01]. Western-blot showed that in the atrium, Kv1.5 and Kv4.2/4.3 expression was down-regulated after 7 days of AF induction, model rats expressed lower levels of Cx40 (P<0.05, vs control) and FKBP12.6 (P<0.05, vs control).CONCLUSION: AF induced by Ach-CaCl₂ injection for 7 days, has a stable AF duration, a shortened AERP with atrium ion channel, connexin and calcium regulation protein down-expressed, all of which related closely to the clinical characterics associated with AF.

Key words: Atrial fibrillation, Electrical remodeling, Connexin 40

中图分类号:

R965.1

汤依群, 孙书娟, 尹月淼, 汪旻晖, 黄潞, 单娇娇. 阵发性房颤诱发大鼠心房肌电重构特征研究[J]. 中国临床药理学与治疗学, 2011, 16(11): 1205-1209.

TANG Yi-qun, SUN Shu-juan, YIN Yue-miao, WANG Min-hui, HUANG Lu, SHAN Jiao-jiao. Characteristics of atrial electrical remodeling in rats induced by paroxysmal atrial fibrillation[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2011, 16(11): 1205-1209.

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