氯沙坦对大鼠离体胸主动脉的舒张作用及机制

中国临床药理学与治疗学 ›› 2011, Vol. 16 ›› Issue (11): 1252-1256.

氯沙坦对大鼠离体胸主动脉的舒张作用及机制

田晶¹, 吕吉元¹, 牛龙刚²

¹山西医科大学第一医院心血管内科,
²山西医科大学药理教研室,太原 030001,山西

收稿日期:2011-08-30 修回日期:2011-10-25 出版日期:2011-11-26 发布日期:2011-11-29
通讯作者: 吕吉元,男,博士,教授,博士生导师,研究方向:冠心病的诊疗。Tel: 13935127530 E-mail: tj182@126.com
作者简介:田晶,女,硕士,医师,研究方向:高血压治疗。Tel: 13935176001 E-mail: tj182@chinaren.com
基金资助:
山西省高校科技开发项目(2006107)

Effects of losartan on vascular tone of isolated thoracic aortic rings in rats

TIAN Jing¹, LV Ji-yuan¹, NIU Long-gang²

¹Department of Cardiology, First Hospital Affiliated to Shanxi Medical University,
²Department of Pharmacology, Shanxi Medical University, Taiyuan 030001, Shanxi, China

Received:2011-08-30 Revised:2011-10-25 Online:2011-11-26 Published:2011-11-29

摘要/Abstract

摘要： 目的: 观察氯沙坦对大鼠胸主动脉的舒张作用,并探讨其作用机制。方法: 记录氯沙坦对血栓素A₂(TXA₂)类似物U46619、氯化钾(KCl)预收缩的离体大鼠胸主动脉环作用,观察N-硝基-L-精氨酸甲酯(L-NAME)与吲哚美辛对其作用的影响。结果: 氯沙坦(3×10^-8～3×10^-5 mol/L)对U46619(1 μmol/L)预收缩的主动脉环有内皮依赖性的、浓度依赖性的舒张作用。L-NAME(0.1 mmol/L)可显著抑制氯沙坦的舒血管作用。吲哚美辛(1 μmol/L )对氯沙坦的舒血管作用无明显影响。氯沙坦对KCl(60 mmol/L)诱发的血管收缩无明显影响。结论: 氯沙坦对U46619引起的血管收缩有浓度依赖性的舒张作用,此作用具有内皮依赖性,与内皮产生的一氧化氮(NO)有关,与前列环素(PGI₂)无关。

关键词: 氯沙坦, 胸主动脉, 血管舒张, U46619, 一氧化氮, 前列环素

Abstract: AIM: To investigate the effects of losartan on the reactivity of the aortic rings in male wistar rats.METHODS: The effects of losartan(3×10^-8-3×10^-5 mol/L) on rat isolated thoracic aortic rings' contraction induced by TXA₂ analog U46619(1 μmol/L) and KCl(60 mmol/L)were observed. In addition, the effect of the NO synthase inhibitor L-NAME(0.1 mmol/L) and the cyclooxygenase inhibitor indomethacin(1μmol/L) on losartan were observed.RESULTS: Losartan (3×10^-8-3×10^-5 mol/L) reduced the constriction induced by U46619(1 μmol/L) in a dose-dependent and endothelium-dependent manner. L-NAME(0.1 mmol/L)significantly shifted the concentration-response curves of losartan to the right. Indomethacin (1 μmol/L) did not change the relaxations of losartan. Losartan did not significantly affect the constriction induced by KCl(60 mmol/L).CONCLUSION: Losartan preferentially antagonizes contraction induced by U46619 in a dose-dependent and endothelium-dependent manner. The vasorelaxation of losartan is relevant to the production of nitrogen and not relevant to the production of prostaglandin.

Key words: Losartan, Angiotasis, Vasodilation, U46619, Nitrogen, Prostaglandin

中图分类号:

R965.2

田晶, 吕吉元, 牛龙刚. 氯沙坦对大鼠离体胸主动脉的舒张作用及机制[J]. 中国临床药理学与治疗学, 2011, 16(11): 1252-1256.

TIAN Jing, LV Ji-yuan, NIU Long-gang. Effects of losartan on vascular tone of isolated thoracic aortic rings in rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2011, 16(11): 1252-1256.

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