丹参乙酸镁对人胚肾细胞内血红素加氧酶表达的影响

中国临床药理学与治疗学 ›› 2011, Vol. 16 ›› Issue (5): 481-487.

• 基础研究 • 下一篇

丹参乙酸镁对人胚肾细胞内血红素加氧酶表达的影响

任贤¹, 侯瑞英², 屈健², 沈杰², 周宏灏², 刘昭前²

¹上海绿谷制药有限公司,上海 201304;
²中南大学临床药理研究所,遗传药理学湖南省重点实验室,长沙 410078, 湖南

收稿日期:2011-02-28 修回日期:2011-04-25 发布日期:2011-07-08
通讯作者: 刘昭前,男,博士,教授,博士生导师,研究方向:遗传药理学和临床药理学。Tel:0731-84805380 E-mail:liuzhaoqian63@126.com
作者简介:任贤,女,硕士,研究方向:新药研制与开发。Tel:021-50504988 E-mail:renxian87@163.com
基金资助:
国家自然科学基金项目(30873089);湖南省自然科学基金重点项目(08JJ3058);上海绿谷制药有限公司委托项目

Effects of magnesium lithospermate B on the expression of heme oxygenase-1 in HEK293T cells

REN Xian¹, HOU Rui-ying², QU Jian², SHEN Jie², ZHOU Hong-hao², LIU Zhao-qian²

¹Shanghai Green Valley Pharmaceutical Co., Ltd., Shanghai 201304, China;
²Institute of Clinical Pharmacology, Hunan Key Laboratory of Pharmacogenetics, Central South University, Changsha 410078, Hunan, China

Received:2011-02-28 Revised:2011-04-25 Published:2011-07-08

摘要/Abstract

摘要： 目的: 以人胚肾细胞(HEK293T)为研究对象,进一步证实丹参乙酸镁(magnesium lithospermate B, LAB)对氧化应激下细胞内活性氧(reactive oxygen species, ROS)产生的影响;以血红素加氧酶-1(heme oxygenase, HO-1)为靶点,观察LAB对高糖诱导下HO-1表达的影响。方法: 运用流式细胞仪检测细胞内ROS水平,RT-PCR和Western blotting分别检测高糖和LAB干预下,HEK 293T细胞内HO-1基因mRNA和蛋白表达水平。结果: 高糖和H₂O₂可使HEK293T细胞内ROS产生明显增多,LAB能够明显抑制应激状态时HEK293T细胞内ROS产生。HEK293T细胞给予高糖刺激后,HO-1 mRNA表达和蛋白质表达均上调,于 24 h 达到高峰。HEK293T细胞预先 30 min 给予10、50和 100 mol/L 3个不同浓度的LAB干预后,与高糖对照组比较,HO-1 mRNA和蛋白质表达水平均有显著性的增加。结论: LAB不但可以直接清除氧化应激下细胞内过多的ROS,而且还可以通过激活下游基因HO-1的表达而发挥抗氧化作用。

关键词: 丹参乙酸镁, 活性氧, 血红素加氧酶, 人胚肾细胞

Abstract: AIM: To further confirm the effects of magnesium lithospermate B (LAB) on the expression of heme oxygenase-1 (HO-1) in HEK293T cells induced by high glucose.METHODS: The intracellular reactive oxygen species in cells was determined by flow cytometry .The expression levels of HO-1 mRNA and HO-1 protein in HEK293T cells treated with high glucose and LAB were determined by quantitative reverse transcription polymerase chain reaction (QRT-PCR) and western blotting.RESULTS: Flow cytometry showed that the ROS concentration was increased markedly in HEK293T cells induced by high glucose and H₂O₂ and LAB inhibited the ROS generation of HEK293T cells. The expression levels of HO-1 mRNA and protein were increased significantly in HEK293T cells treated with high glucose and reached the peak value at 24 h.Compared with high glucose group,the expression levels of HO-1 mRNA and protein were obviously enhanced after cells were treated with 10,50,100 mol/L LAB.CONCLUSION: LAB not only can directly clear up the excess ROS in cells under oxidative stress, but also it play a antioxidant role via an increase of downstream gene HO-1 expression.

Key words: Magnesium lithospermate B, Reactive oxygen, Heme oxygenase-1, HEK293T

中图分类号:

R965.2

任贤, 侯瑞英, 屈健, 沈杰, 周宏灏, 刘昭前. 丹参乙酸镁对人胚肾细胞内血红素加氧酶表达的影响[J]. 中国临床药理学与治疗学, 2011, 16(5): 481-487.

REN Xian, HOU Rui-ying, QU Jian, SHEN Jie, ZHOU Hong-hao, LIU Zhao-qian. Effects of magnesium lithospermate B on the expression of heme oxygenase-1 in HEK293T cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2011, 16(5): 481-487.

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丹参乙酸镁对人胚肾细胞内血红素加氧酶表达的影响

Effects of magnesium lithospermate B on the expression of heme oxygenase-1 in HEK293T cells

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