p-CREB在激动GHB受体对大鼠局灶性脑缺血再灌注损伤的保护作用中的变化

中国临床药理学与治疗学 ›› 2011, Vol. 16 ›› Issue (5): 488-491.

p-CREB在激动GHB受体对大鼠局灶性脑缺血再灌注损伤的保护作用中的变化

杨其侠^1,2, 秦浩², 谷淑玲¹

¹徐州医学院药理学教研室, 徐州 221002,江苏;
²山东枣庄市立医院药剂科,枣庄 277102,山东

收稿日期:2011-02-08 修回日期:2011-04-06 发布日期:2011-07-08
通讯作者: 谷淑玲,女,本科,研究方向:心脑血管药理学。Tel:0516-85748484 E-mail:gushling@163.com
作者简介:杨其侠,女,硕士,研究方向:心脑血管药理学。Tel:13561128202 E-mail:yangqixia1980@126.com

Change of p-CREB in the protection effect of agitating gamma-hydroxybutyric acid receptor on focal cerebral ischemia-reperfusion injury in rats

YANG Qi-xia^1,2, QIN Hao², GU Shu-ling¹

¹Department of Pharmacology, Xuzhou Medical College, Xuzhou 221002, Jiangsu, China;
²Zaozhuang Municipal Hospital, Zaozhuang, 277102, Shangdong, China

Received:2011-02-08 Revised:2011-04-06 Published:2011-07-08

摘要/Abstract

摘要： 目的: 研究磷酸化环磷酸腺苷反应蛋白结合元件(p-CREB)在激动γ-羟基西酸(GHB)受体对大鼠局灶性脑缺血再灌注损伤的保护作用中的变化。方法: SD大鼠随机分为Sham组,I/R组,NCS-356 160、320、640 μg/kg 组。采用改良的Longa线栓法缺血2 h/再灌2 h建立I/R模型。分别于缺血前 30 min 和再灌前icv给予药物;5分制评分法评价大鼠神经功能。再灌 24 h 后免疫组织化学法测定p-CREB的表达。结果: I/R组大鼠行为学评分为 3.2±0.7,明显高于Sham组(n=8,P<0.01);NCS-356 640 μg/kg 组的行为学评分则明显低于I/R组(n=8,P<0.05)。I/R组大鼠缺血区脑皮层细胞核内p-CREB的表达为 96.3±6.0,明显高于Sham组(n=8,P<0.01);NCS-356 320 μg/kg 组和NCS-356 640 μg/kg 组脑缺血皮层细胞核内p-CREB的表达与I/R组比较明显降低(n=8,P<0.01)。结论: 激动GHB受体对大鼠局灶性脑缺血再灌注损伤具有一定的保护作用,且缺血组织p-CREB的表达在此保护作用中降低。

关键词: γ-羟基丁酸受体, 大鼠, 脑缺血再灌注, 磷酸化环磷酸腺苷反应蛋白结合元件

Abstract: AIM: To investigate the change of p-CREB in the protection effect of agitating gamma-hydroxybutyric acid receptor on focal cerebral ischemia-reperfusion injury in rats.METHODS: The Sprague-Dawley rats were randomly divided into five groups: sham operation group(Sham), ischemia-reperfusion group(I/R), NCS-356 160,320,640 μg/kg group.The middle cerebral artery occlusion (MCAO) model referring to Longa's method with modifications was adopted. These groups were administered NCS-356 in right lateral cerebral ventricle. Behavioral consequence of MCAO rats was studied 2 h after ischemia reperfusion. After 24 h of ischemia reperfusion, rats' ischemic cortex were used to measure the phosphorylation cAMP reactive protein binding element (p-CREB) by immunohistochemical method.RESULTS: The ethology score in rats of I/R group was 3.2±0.7,compared with the Sham group, it was increased statistically (n=8, P<0.01) The neurological function score in NCS 640 group was obviously lower than that in I/R group (n=8,P<0.05). The p-CREB gray scale value in I/R group was 96.3±6.0 ,which was higher than that in Sham group(n=8,P<0.01). The p-CREB gray scale value in NCS 640 group and NCS 320 group was obviously lower than that in I/R group(n=8,P<0.01).CONCLUSION: Activating GHBR has protective effects on focal cerebral ischemia-reperfusion injury in rats.The expresstion of p-CREB is reduced in the protection effect of agitating gamma-hydroxybutyric acid receptor on focal cerebral ischemia-reperfusion injury in rats.

Key words: Gamma-hydroxybutyric acid receptor, Rat, Cerebral ischemia-reperfusion, Phosphorylation cAMP reactive protein binding element

中图分类号:

杨其侠, 秦浩, 谷淑玲. p-CREB在激动GHB受体对大鼠局灶性脑缺血再灌注损伤的保护作用中的变化[J]. 中国临床药理学与治疗学, 2011, 16(5): 488-491.

YANG Qi-xia, QIN Hao, GU Shu-ling. Change of p-CREB in the protection effect of agitating gamma-hydroxybutyric acid receptor on focal cerebral ischemia-reperfusion injury in rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2011, 16(5): 488-491.

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