关键词: 维生素K₂, 苯那普利, 细胞凋亡, 血管内皮生长因子, 胃癌

Abstract: AIM: To investigate the effect of vitamin K₂ combined with benazepril on human gastric carcinoma SGC-7901 cells in vitro. METHODS: SGC-7901 cells at logarithmic growth phase were obtained and were divided into 5 groups: negative control group, blank control group, vitamin K₂ therapy group, benazepril therapy group and combination therapy group(vitamin K₂+benazepril). SGC-7901 cells were cultured with drugs of different concentrations (vitamin K₂: 5, 10, 20, 40, 80 μmol/L)or benazepril (benazepril: 0.625, 1.25, 2.5, 5, 10 μg/mL) or vitamin K₂+benazepril for 24, 48 and 72 h in drugs group. Cells cultured without drugs were served as negative control and blank control group without cells. The inhibition rates of SGC-7901 cells were detected by MTT assay, apoptosis rate of SGC-7901 cells was determined by Annexin V/PI double staining flow cytometry and DNA ladder, cell cycles of SGC-7901 cells were analysed by flow cytometry and VEGF expression of SGC-7901 cells were detected with RT-PCR. RESULTS: Vitamin K₂ or benazepril had significant inhibitory effect on SGC-7901 cells growth, but combination group was more effective than the drug group alone on SGC-7901 cells proliferation, apoptosis and VEGF expression. CONCLUSION: Vitamin K₂ combined with benazepril have obvious coordination therapeutic effect. The proliferation of SGC-7901 cells could be inhibited by inducing apoptosis and inhibiting VEGF expression.

Key words: Vitamin K₂, Benazepril, Apoptosis, VEGF, Gastric carcinoma