CDA-Ⅱ抑制肺腺癌细胞增殖及C-erbB2表达的实验研究

中国临床药理学与治疗学 ›› 2011, Vol. 16 ›› Issue (8): 847-851.

CDA-Ⅱ抑制肺腺癌细胞增殖及C-erbB2表达的实验研究

王红艳¹, 涂永生², 谢晓斌¹

¹广州医学院病理教研室, ²生理教研室,广州 510182,广东

收稿日期:2011-06-23 修回日期:2011-07-19 出版日期:2011-08-26 发布日期:2011-09-07
作者简介:王红艳,女,硕士,讲师,研究方向:肿瘤病理。Tel: 020-81340347 E-mail: wanghongyan75@126.com
基金资助:
广东省医学科研基金项目(B2007088);广州医学院科学研究项目(2006ZR016)

Experimental study of proliferation and C-erbB2 expression of lung cancer cells induced by cell differentiation agent-Ⅱ

WANG Hong-yan¹, TU Yong-sheng², XIE Xiao-bin¹

¹Department of Pathology, Guangzhou Medical University, ²Department of Physiology, Guangzhou 510182, Guangdong, China

Received:2011-06-23 Revised:2011-07-19 Online:2011-08-26 Published:2011-09-07

摘要/Abstract

摘要： 目的: 研究细胞分化剂 CDA-Ⅱ(cell differentiation agent-Ⅱ) 抑制人肺腺癌细胞A549增殖及对癌基因C-erbB2表达的影响。方法: 不同浓度的CDA-Ⅱ作用人肺癌细胞A549,用CCK-8法检测细胞增殖抑制率;RT-PCR和Western blot 检测经CDA-Ⅱ作用后A549细胞C-erbB2表达的改变。结果: CDA-Ⅱ能明显抑制肺腺癌细胞增殖,且抑制效应呈剂量依赖性。C-erbB2在CDA-Ⅱ抗人肺癌细胞A549增殖中表达下调。结论: CDA-Ⅱ可有效抑制人肺癌细胞A549增殖,该作用可能与抑制C-erbB2的信号传导通路有关。

关键词: CDA-Ⅱ, 人肺癌A549细胞, 增殖, C-erbB2

Abstract: AIM: To investigate the effects of CDA-Ⅱ (Uroacitide) which is a healthy human urine extraction on proliferation and C-erbB2 expression of human lung cancer cell line A549.METHODS: The effect of CDA-Ⅱ on cellular viability was determined by CCK-8 assay.The change of C-erbB2 expression in A549 cells induced by CDA-Ⅱ was detected by RT-PCR and Western blot.RESULTS: After A549 cells were treated with various concentration of CDA-Ⅱ, cell proliferation was significantly inhibited in a dose-dependent manner.The expression of C-erbB2 was downregulated in A549 cells induced by CDA-Ⅱ.CONCLUSION: CDA-Ⅱ can inhibit the growth of A549 cells associated with downregulation of C-erbB2, which indicates that C-erbB2 is involved in CDA-Ⅱ induced proliferation inhibition of A549 cells.

Key words: CDA-Ⅱ, Human lung cancer cell line A549, Proliferation, C-erbB2

中图分类号:

R965.2

王红艳, 涂永生, 谢晓斌. CDA-Ⅱ抑制肺腺癌细胞增殖及C-erbB2表达的实验研究[J]. 中国临床药理学与治疗学, 2011, 16(8): 847-851.

WANG Hong-yan, TU Yong-sheng, XIE Xiao-bin. Experimental study of proliferation and C-erbB2 expression of lung cancer cells induced by cell differentiation agent-Ⅱ[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2011, 16(8): 847-851.

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