中国汉族儿童CYP3A5*3基因型与急性淋巴细胞白血病易感性的关联研究

中国临床药理学与治疗学 ›› 2012, Vol. 17 ›› Issue (1): 73-77.

中国汉族儿童CYP3A5^*3基因型与急性淋巴细胞白血病易感性的关联研究

刘艳¹, 陆晓彤¹, 盛琦², 田怀平¹, 徐阿晶¹, 唐跃年¹, 卜书红¹, 李莉霞¹, 张健¹

¹上海交通大学医学院附属新华医院药剂科,上海 200092;
²儿童血液肿瘤科, 上海 200092

收稿日期:2011-08-25 修回日期:2011-11-25 出版日期:2012-01-26 发布日期:2012-02-16

Association of CYP3A5^*3 with Acute Lymphoblastic Leukemia susceptiveness in Chinese Han children

LIU Yan¹, LU Xiao-tong¹, SHENG Qi², TIAN Huai-ping¹, XU A-jing, TANG Yue-nian¹ , BU Shu-hong¹, LI Li-xia¹, ZHANG Jian¹

¹Department of Pharmacy, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine;
²Department of Pediatric Hematology and Oncology, Shanghai 200092, China

Received:2011-08-25 Revised:2011-11-25 Online:2012-01-26 Published:2012-02-16
Contact: Zhang Jian, Male, Master, Supervisor of master, Chief Pharmcist. Researh field: Hospital Pharmacy. Tel: 021-25077150 E-mail: zj_xhpharm@126.com
About author:Liu Yan,Female, Master, Pharmacist-in-charge. Researh field: Hospital Pharmacy,Pharmacogenetics. Tel: 021-25077156 E-mail: liuyan160@163.com
Supported by:
Research Award Fund for out Standing Young Teachers of Shanghai;Project suported by the Shanghai Health Bureau, China(2009068)

摘要/Abstract

摘要： 目的: 在中国汉族儿童中探讨白血病易感性与CYP3A5^*3的频率之间的相关性。方法: 共收集172名中国汉族儿童血液,包括52名急性白血病患者(27女性,25男性)和120名健康儿童(53女性,67男性)。提取DNA,然后用PCR-RFLP方法检测基因型。结果: CYP3A5^*3在人群中的分布符合Hardy-Weinberg 平衡。儿童白血病中CYP3A5^*3频率为0.721(95% CI: 0.549, 0.893),而健康儿童中为0.758 (95% CI: 0.674, 0.842),两者之间无明显差异,并且也无男女性别差异。结论: CYP3A5^*3是健康儿童和白血病患者中的常见基因型,健康儿童和白血病患儿中CYP3A5^*3频率一致, 因此CYP3A5^*3与儿童白血病易感性之间无关联。

关键词: 儿童急性淋巴细胞白血病(ALL), CYP3A5^*3, 遗传变异

Abstract: To investigate the association of CYP3A5^*3 with Acute Lymphoblastic Leukemia (ALL) susceptiveness in Chinese Han children.METHODS: CYP3A5 genotype was analyzed from 172 Chinese han children, including 52 ALL patients(27 female and 25 male) and 120 healthy controls(80 female and 92 male).Genomic DNA was extracted from blood sample, and PCR-RFLP was used to identify the polymorphism.RESULTS: This polymorphism were matched to Hardy-Weinberg equilibrium in all group.The allelic frequency of CYP3A5^*3 was 0.721 (95% CI: 0.549, 0.893) in patients while 0.758 (95% CI: 0.674, 0.842) in healthy controls. There was no significant difference existed in CYP3A5^*3 frequencies between patients and controls (P=0.275), males and females (P=0.432) in the total population.CONCLUSION: The CYP3A5^*3 variant represents a common polymorphism in Chinese healthy children and ALL, and this polymorphism is poor association with ALL susceptiveness in children.

Key words: Acute Lymphoblastic Leukemia (ALL), CYP3A5^*3, Polymorphism

中图分类号:

R968

刘艳, 陆晓彤, 盛琦, 田怀平, 徐阿晶, 唐跃年, 卜书红, 李莉霞, 张健. 中国汉族儿童CYP3A5^*3基因型与急性淋巴细胞白血病易感性的关联研究[J]. 中国临床药理学与治疗学, 2012, 17(1): 73-77.

LIU Yan, LU Xiao-tong, SHENG Qi, TIAN Huai-ping, XU A-jing, TANG Yue-nian , BU Shu-hong, LI Li-xia, ZHANG Jian. Association of CYP3A5^*3 with Acute Lymphoblastic Leukemia susceptiveness in Chinese Han children[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(1): 73-77.

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