碘化N-正丁基氟哌啶醇抑制兔颈总动脉球囊损伤后血管内膜和平滑肌细胞增殖的作用研究

中国临床药理学与治疗学 ›› 2012, Vol. 17 ›› Issue (10): 1112-1117.

碘化N-正丁基氟哌啶醇抑制兔颈总动脉球囊损伤后血管内膜和平滑肌细胞增殖的作用研究

黄展勤, 姜红岩, 李金玉, 刘幸平, 郑燕珊, 高分飞, 陈一村, 石刚刚

汕头大学医学院药理学教研室,汕头 515041,广东

收稿日期:2012-06-15 修回日期:2012-09-13 发布日期:2012-10-19
通讯作者: 石刚刚,通信作者,男,博士,教授,博导,研究方向:心血管药理学与新药开发。 E-mail: ggshi@stu.edu.cn
作者简介:黄展勤,男,博士,副教授,硕导,研究方向:心血管药理学。Tel: 0754-88900432 E-mail: zqhuang@stu.edu.cn
基金资助:
国家自然科学基金资助项目(30901810); 广东省自然科学基金资助项目(9151063201000072)

Effects of N-n-butyl haloperidol iodide on the proliferation of intima and vascular muscle cells of rabbit carotid artery after balloon injury

HUANG Zhan-qin, JIANG Hong-yan, LI Jin-yu, LIU Xing-pin, ZHENG Yan-shan, GAO Fen-fei, CHEN Yi-cun, SHI Gang-gang

Department of Pharmacology, Shantou University Medical College, Shantou 515041, Guangdong, China

Received:2012-06-15 Revised:2012-09-13 Published:2012-10-19

摘要/Abstract

摘要： 目的: 探讨碘化N-正丁基氟哌啶醇(F₂)对兔颈总动脉球囊损伤后内膜及平滑肌细胞增殖的影响。方法: 30只新西兰兔随机分为假手术组、模型组、F₂高剂量给药组(2 mg/kg)、 F₂中剂量给药组(1 mg/kg)、F₂低剂量给药组(0.5 mg/kg)。模型组及F₂各给药组行左侧颈总动脉球囊损伤,各组分别于术后 7 d 取颈总动脉段,常规病理切片,HE染色,免疫组化法测定α-actin、增殖细胞核抗原(PCNA)的表达水平。结果: 与假手术组比较,模型组术后 7 d 血管内膜厚度、内膜面积、内膜厚度/中膜厚度、内膜面积/中膜面积、血管壁细胞PCNA表达显著增加(P<0.01),F₂剂量依赖地降低上述指标。与假手术组比较,模型组血管壁α-actin阳性染色减少,F₂各给药组可增加血管壁平滑肌细胞中α-actin阳性染色率。结论: F₂能抑制兔颈总动脉机械损伤后血管内膜和平滑肌细胞的增殖。

关键词: 碘化N-正丁基氟哌啶醇, 机械损伤, 血管内膜, 血管平滑肌细胞, 增殖

Abstract: AIM: To investigate the effects of N-n-butyl haloperidol iodide (F₂) on the proliferation of intima and vascular smooth muscle cells (VSMCs) of the rabbit carotid artery after balloon injury. METHODS: 30 New Zealand rabbits were randomly divided into Sham group, Model group, F₂ high-dose treatment group (2 mg/kg), F₂ medium-dose treatment group (1 mg/kg), F₂ low-dose treatment group (0.5 mg/kg). After 7 days, experimental segments of arteries were harversted and subsequently progressed routine pathological sections, hematoxylin-eosin staining, PCNA expression was measured by immunohistochemical staining. RESULTS: Compared with the sham group, the intimal thickness, intimal area, intimal/medial thickness and intima-to-media area ratio of the Model group significantly increase (P<0.01). F₂ caused a dose-dependent reduction in these indicators. F₂ dose-dependently decrease the PCNA positive rate of VSMCs in the vascular wall as compared with Model group (P<0.01). CONCLUSION: F₂ inhibits the proliferation of intima and VSMCs of rabbit carotid artery after balloon injury.

Key words: N-n-butyl haloperidol iodide, Mechanical injury, Intima, Vascular smooth muscle cells, Proliferation

中图分类号:

R965.2

黄展勤, 姜红岩, 李金玉, 刘幸平, 郑燕珊, 高分飞, 陈一村, 石刚刚. 碘化N-正丁基氟哌啶醇抑制兔颈总动脉球囊损伤后血管内膜和平滑肌细胞增殖的作用研究[J]. 中国临床药理学与治疗学, 2012, 17(10): 1112-1117.

HUANG Zhan-qin, JIANG Hong-yan, LI Jin-yu, LIU Xing-pin, ZHENG Yan-shan, GAO Fen-fei, CHEN Yi-cun, SHI Gang-gang. Effects of N-n-butyl haloperidol iodide on the proliferation of intima and vascular muscle cells of rabbit carotid artery after balloon injury[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(10): 1112-1117.

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