地塞米松对顺铂诱导的HepG2细胞凋亡及Bcl-2和Caspase-3蛋白表达的影响

中国临床药理学与治疗学 ›› 2012, Vol. 17 ›› Issue (10): 1118-1123.

地塞米松对顺铂诱导的HepG₂细胞凋亡及Bcl-2和Caspase-3蛋白表达的影响

谭俊^1,2, 阮冰¹, 周密³, 高国生²

¹浙江大学医学院附属第一医院感染性疾病诊治国家重点实验室, 杭州 310003,浙江;
²浙江宁波市第二医院肝病科,宁波 315016,浙江;
³宁波大学医学院微免教研室,宁波 315000, 浙江

收稿日期:2012-02-24 修回日期:2012-06-12 发布日期:2012-10-19
通讯作者: 谭俊,通信作者,男,硕士,主治医师,主要从事肝脏疾病的基础与临床研究。Tel: 13867854770 E-mail: tj790613@sina.com
基金资助:
浙江省医药卫生一般研究计划(2011KYB107)

Effects of Dexamethasoneon apoptosis of HepG2 cells induced by Cisplatin and expression of Bcl-2 and Caspase-3

TAN Jun^{1, 2}, RUAN Bing¹, ZHOU Mi³, GAO Guo-sheng²

¹Department of Infectious Diseases, National Key Laboratory for Diagnosis and Treatment of Infectious Diseases in China, the First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, Zhejiang, China;
²Department of Liver Diseases, Ningbo No.2 Hospital, Ningbo 315016, Zhejiang, China;
³Department of Microbiology and Immunology, Medical College, Ningbo University, Ningbo 315211, Zhejiang, China

Received:2012-02-24 Revised:2012-06-12 Published:2012-10-19

摘要/Abstract

摘要： 目的: 探讨地塞米松对顺铂诱导HepG2细胞凋亡的影响,并观察Bcl-2和Caspase-3蛋白的表达。方法: HepG2细胞与顺铂及不同浓度的地塞米松共培养,RT-PCR检测Bcl-2、Caspase-3的mRNA表达,Western blot检测Bcl-2、Caspase-3蛋白表达,流式细胞术(FACS)检测各组HepG2细胞的凋亡情况。结果: 随着地塞米松浓度的增加,HepG2细胞中Bcl-2蛋白表达量升高,Caspase-3蛋白表达量下降。同时细胞凋亡率降低。结论: 地塞米松部分通过Bcl-2途径抑制肝癌细胞的凋亡,而Caspase-3又在调控细胞凋亡中起重要作用。

关键词: 地塞米松, 顺铂, HepG2细胞, 凋亡

Abstract: AIM: To investigate the mechanism of Dexamethasone (DEX) on apoptosis of HepG2 cells induced by Cisplatin (DDP) and the expression of Bcl-2 and Caspase-3. METHODS: HepG2 and DDP were co-cultured, a serials of concentrations DEX were added into it, the expression of mRNA and protein of Bcl-2 and Capase-3 were analyzed separately by RT-PCR and Western blot,and the percentage of cell apoptosis was analyzed by FACS. RESULTS: With DEX increases in concentration, the expression of Bcl-2 was increased and expression of Caspase-3 was decreased correspondently, the rate of apoptosis of HepG2 descends clearly.CONCLUSION: DEX restrains apoptosis of HepG2 cells induced by Cisplatin (DDP) partly through Bcl-2 anti-apoptosis pathways, and Caspase-3 plays an important role in regulating the cell apoptosis.

Key words: Dexamethasone, Cisplatin, HepG2 cells, Apoptosis

中图分类号:

R965.2

谭俊, 阮冰, 周密, 高国生. 地塞米松对顺铂诱导的HepG₂细胞凋亡及Bcl-2和Caspase-3蛋白表达的影响[J]. 中国临床药理学与治疗学, 2012, 17(10): 1118-1123.

TAN Jun, RUAN Bing, ZHOU Mi, GAO Guo-sheng. Effects of Dexamethasoneon apoptosis of HepG2 cells induced by Cisplatin and expression of Bcl-2 and Caspase-3[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(10): 1118-1123.

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地塞米松对顺铂诱导的HepG₂细胞凋亡及Bcl-2和Caspase-3蛋白表达的影响

Effects of Dexamethasoneon apoptosis of HepG2 cells induced by Cisplatin and expression of Bcl-2 and Caspase-3

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