HPLC-UV法测定大鼠体内S-腺苷蛋氨酸浓度及其药动学研究

中国临床药理学与治疗学 ›› 2012, Vol. 17 ›› Issue (12): 1372-1377.

HPLC-UV法测定大鼠体内S-腺苷蛋氨酸浓度及其药动学研究

朱丽丽¹, 章礼久¹, 方海明¹, 姚婷婷¹, 杨姣¹, 王佳佳², 夏泉³

¹安徽医科大学第二附属医院消化内科,合肥 230601,安徽;
²安徽医科大学药理学教研室,合肥 230022,安徽;
³安徽医科大学第一附属医院药剂科,合肥 230022,安徽

收稿日期:2012-07-23 修回日期:2012-10-30 发布日期:2012-12-31
通讯作者: 章礼久,通信作者,男,主任医师,硕士生导师,研究方向:消化系疾病药物治疗学。E-mail: zhanglijiu6336@163.com
作者简介:朱丽丽,女,硕士研究生,研究方向:消化系疾病药物治疗学。Tel: 13637083780 E-mail: lilizhu615@163.com
基金资助:
安徽省省高校优秀青年人才基金重点项目(2010SQRL075ZD); 安徽省省教育厅自然科学基金项目(KJ2010B390; KJ2011Z191)

Determination and pharmacokinetics of S-Adenosylmethionine in rats plasma by HPLC-UV

ZHU Li-li¹, ZHANG Li-jiu¹, FANG Hai-ming¹, YAO Ting-ting¹, YANG Jiao¹, WANG Jia-jia², XIA Quan³

¹Department of Gastroenterology, the Second Hospital of Anhui Medical University, Hefei 230601, Anhui, China;
²Department of Pharmacology, Anhui Medical University, Hefei 230022, Anhui, China;
³Department of Pharmacy, the First Hospital of Anhui Medical University, Hefei 230022, Anhui, China

Received:2012-07-23 Revised:2012-10-30 Published:2012-12-31

摘要/Abstract

摘要： 目的: 建立HPLC-UV法测定大鼠血浆中S-腺苷蛋氨酸(S-Adenosylmethionine, SAMe)浓度,并用此法研究静脉给药后的大鼠体内药代动力学特征。方法: 色谱柱:大连依利特Kromasil C₁₈ 柱(4.6 mm×200 mm, 5 μm),流动相:甲醇∶0.3%三氟乙酸溶液(含1%磷酸二氢钠盐)=2∶98,等浓度洗脱,紫外检测波长:260 nm,流速:0.6 mL/min,柱温:30 ℃。大鼠(n=6)经静脉给予 140 mg/kg SAMe并测定血浆药物浓度,采用DAS 2.0 软件进行药动分析。结果: SAMe能完全分离,线性范围:10~2000 μg/mL,回归方程为Y=30241X+256204,r=0.9991, 日内精密度分别为 2.6%,3.6%,3.9%;日间精密度分别为 6.3%,4.9%,7.9%;回收率分别为107%,101%,98.3%。静脉给予 140 mg/kg SAMe药动学参数,血浆药物浓度-时间曲线下面积AUC_(0-t)为(569.52±174.87) mg·L^-1·h,半衰期(t_1/2)为(2.03±0.96) h,清除率(CL)为(0.21±0.13)L·h^-1·kg^-1,表观分布容积(V_d)为(0.53±0.31) L/kg。结论: 本实验方法专属性好,灵敏度高,可靠性强,可用于SAMe药动学分析。

关键词: S-腺苷蛋氨酸, HPLC-UV, 药代动力学

Abstract: AIM: To establish an HPLC-UV assay for determining plasma concentration of S-Adenosylmethionine (SAMe) and to study its pharmacokinetics in rats. METHODS: The chromatographic conditions were as follows: a Kromasil C₁₈ column (4.6 mm×200 mm , 5 μm) ,a flow rate of 0.6 mL/min for the mobile phase of methanol/0.3% trifluoroacetic acid (V/V) (dissolve 1% phosphate buffer) (2∶98) , an isocratic elution mode was carried out at column temperature (30 ℃) and the UV detection at the wavelength of 260 nm. 140 mg/kg SAMe was administrated by intravenous and plasma concentration were determined by HPLC-UV assay. RESULTS: SAMe was successfully separated. The calibration curve showed a good linear regression within the range of 10-2000 μg/mL. The regression equation was Y=30241X+256204, r=0.9991. The precision of intra-assay and inter-assay were 2.6%, 3.6%, 3.9% and 6.3%, 4.9%, 7.9%, respectively. The average extraction recovery was 107%, 101%, 98.3%, respectively. The pharmacokinetic parameter were AUC_(0-t) (569.52±174.87) mg·L^-1·h,t_1/2 (2.03±0.96) h, CL (0.21±0.13) L·h^-1·kg^-1, V_d (0.53±0.31) L/kg, respectively.CONCLUSION: This method was proved to be simple and efficient with excellent specificity, sensitivity, precision, recovery and can be applied to pharmacokinetic investigation of SAMe.

Key words: S-Adenosylmethionine, HPLC-UV, Pharmacokinetics

中图分类号:

R969.1
R917

朱丽丽, 章礼久, 方海明, 姚婷婷, 杨姣, 王佳佳, 夏泉. HPLC-UV法测定大鼠体内S-腺苷蛋氨酸浓度及其药动学研究[J]. 中国临床药理学与治疗学, 2012, 17(12): 1372-1377.

ZHU Li-li, ZHANG Li-jiu, FANG Hai-ming, YAO Ting-ting, YANG Jiao, WANG Jia-jia, XIA Quan. Determination and pharmacokinetics of S-Adenosylmethionine in rats plasma by HPLC-UV[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(12): 1372-1377.

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