鼠神经生长因子诱导骨髓间充质干细胞向神经元样细胞分化的实验研究

中国临床药理学与治疗学 ›› 2012, Vol. 17 ›› Issue (2): 137-140.

鼠神经生长因子诱导骨髓间充质干细胞向神经元样细胞分化的实验研究

陈再丰, 许信龙, 魏晓捷, 傅小君, 潘红松, 谢青松

浙江省慈溪市人民医院神经外科,慈溪 315300,浙江

收稿日期:2011-11-24 修回日期:2012-01-13 出版日期:2012-02-26 发布日期:2012-03-12
作者简介:陈再丰,男,硕士,主治医师,主要从事颅脑外伤的基础与临床工作。Tel: 0574-63929103 E-mail: czf020@yahoo.com.cn
基金资助:
慈溪市科技局资助项目(CN2010021)

Differentiation of rat bone marrow stromal cells into neurol cells induced by mouse nervous growth factor

CHEN Zai-feng, XU Xin-long, WEI Xiao-jie, FU Xiao-jun, PAN Hong-song, XIE Qin-song

Department of Neurosurgery, Cixi Municipal People's Hospital, Cixi 315300, Zhejiang, China

Received:2011-11-24 Revised:2012-01-13 Online:2012-02-26 Published:2012-03-12

摘要/Abstract

摘要： 目的: 观察鼠神经生长因子(mNGF)对骨髓间充质干细胞(BMSCs)向神经细胞分化的影响。方法: 4周龄健康SD大鼠60只,按随机数字表法分为治疗组(n=30)和正常对照组(n=30)。治疗组在常规培养的基础上加入浓度为 0.1 μg/mL mNGF作为诱导液。将培养的第3代BMSCs制成单细胞悬液,诱导后3 d,观察细胞形态变化,行神经元特异性烯醇酶(NSE) 免疫荧光检测,计算阳性率。结果: 正常对照组细胞仅有少量NSE的表达,治疗组细胞表达NSE阳性率较对照组细胞显著增加(P＜0.01)。结论: mNGF可诱导大鼠BMSCs向神经元样细胞分化。

关键词: 鼠神经生长因子, 骨髓间充质干细胞, 细胞分化

Abstract: AIM: To observe the effect of mouse nervous growth factor(mNGF) on differentiation of rat bone marrow stromal cells into neurol cells.METHODS: 60 4-week-old healthy clean SD rats were equally randomized into divided into treatment group(n=30)and normal control group(n=30). The treatment group received 0.1 μg/mL mNGF as induction medium. The rats BMSCs of passage 3 were cultured in the medium with or without previous brain tissue extracts. The expressions of neuron specific encolase (NSE) were detected after 3 days by immunocytochemistry.RESULTS: There were a few NSE in the normal control group. The expression of NSE of treatment group were significantly higher than normal control group(P＜0.01).CONCLUSION: mNGF can induce BMSCs into neural cells.

Key words: Mouse nervous growth factor, Bone marrow stromal cells, Cell differentiation

中图分类号:

R965.2

陈再丰, 许信龙, 魏晓捷, 傅小君, 潘红松, 谢青松. 鼠神经生长因子诱导骨髓间充质干细胞向神经元样细胞分化的实验研究[J]. 中国临床药理学与治疗学, 2012, 17(2): 137-140.

CHEN Zai-feng, XU Xin-long, WEI Xiao-jie, FU Xiao-jun, PAN Hong-song, XIE Qin-song. Differentiation of rat bone marrow stromal cells into neurol cells induced by mouse nervous growth factor[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(2): 137-140.

参考文献

[1] Kim S, Honmou O, Kato K, et al. Neural differentiation potential of peripheral blood-and bone-marrow-derived precursor cells[J]. Brain Res, 2006, 11(23):27-33.
[2] Kawaja MD,Smithson LJ,Elliott J,et al. Nerve growth factor promoter activity revealed in mice expressing enhanced green fluorescent protein[J]. J Comparative Neurology, 2011, 519(9): 1631-1821.
[3] Ye X, Asim M, Michael C, et al. Emerging treatments for traumatic brain injury[J]. Expert Opin Emerg Drugs, 2009, 14(1): 67-84.
[4] Takagi Y, Takahashi J, Saiki H, et al. Dopaminergic neurons generated from monkey embryonic stem cells function in a Parkinson primate model[J]. J Clin Invest, 2005, 115(1): 102-109.
[5] Bang OY, Lee JS, Lee PH, et al. Autologous mesenchymal stem cell transplantation in stroke patients[J]. Ann Neurol, 2005, 57(6): 874-882.
[6] D'Ipplito G, Diabira S, Howard GA, et al. Marrow-isolated adult multilineage inducible (MIAMI) cells, a unique population of postnatal young and old human cells with extensive expansion and differentiation potential[J]. J Cell Sci, 2004, 117(14): 2971-2981.
[7] 陈晓晨,吴德沛,陈峰,等. 亚砷酸对人骨髓间充质干细胞增值及凋亡的影响[J]. 中国临床药理学与治疗学,2007,12(6):635-638.
[8] Kopen GC, Prockop DJ, Phinney DG. Marrow stromal cells migrate throughout forebrain and cerebellum, and they differentiate into astrocytes after injection into neonataI mouse brains[J]. Proc Natl Acad Sci USA, 1999, 96(19): 10711-10716.
[9] Rivera FJ, Sierralta WD, Minguell JJ, et al. Adult hippocampus derived soluble factors induce a neuronal-like phenotype in mesenchymal stem cells[J]. Neurosd Lett, 2006, 406(1): 49-54.
[10] Sagisaka T, Matsukawa N, Toyoda T, et al. Directed negraI lineage differentiation of adult hippocampal progenitor cells via modulation of hippocampaI cholinergic neurostimulating peptide precursor expression [J]. Brain Res, 2010,1327: 107-117.
[11] Li Y, Chopp M. Marrow stromal cell transplantation in stroke and traumatic brain injury[J]. Neurosci Lett, 2009, 456(3): 120-123.
[12] Tang XQ, Cai J, Nelson KD, et al. Functional repair after dorsal root rhizotomy using nerve conduits and neurotrophic molecules[J]. Eur J Neurosci, 2004,20(5): 121-125.
[13] Letournel F,Bocquet A, Perrot R,et al.Neurofilament high molecular weight-green fluorescent protein fusion is normally expressed in neurons and transported in axons: A neuronal-marker to investigate the biology of neurofilaments[J]. Neuroscience, 2006,137(1):103-111.
[14] Zhang Y, Zhu Z, Yang W, et al. An emerging recombinant human enterovirus 71 responsible for the 2008 outbreak of hand foot and mouth disease in Fuyang city of China[J]. Virology J, 2010,7(1): 94-96.