低聚葡萄籽原花青素对异丙肾上腺素诱导心肌细胞肥大的影响

中国临床药理学与治疗学 ›› 2012, Vol. 17 ›› Issue (5): 497-501.

低聚葡萄籽原花青素对异丙肾上腺素诱导心肌细胞肥大的影响

高杉¹, 黄春霞², 黄玲玲¹, 俞婷婷¹, 汪星辉¹, 张野²

¹安徽医科大学基础医学院药理学教研室,合肥 230032,安徽;
²安徽医科大学第二附属医院麻醉科,合肥 230061,安徽

收稿日期:2012-02-22 修回日期:2012-04-26 出版日期:2012-05-26 发布日期:2012-05-28
作者简介:高杉,男,博士,副教授,硕士生导师,研究方向:心血管药理学。Tel: 13083495662 E-mail: aydgs@126.com
基金资助:
教育部高等学校博士学科点基金(20103420120002);国家自然科学基金(81073088);安徽医科大学博士启动基金(XJ200709)

Effect of oligomeric grape seed proanthocyanidins on prevention isoproterenol induced cardiomyocytes hypertrophy

GAO Shan¹, HUANG Chun-xia², HUANG Ling-ling¹, YU Ting-ting¹, WANG Xing-hui¹, ZHANG Ye²

¹Department of Pharmacology, Basic College, Anhui Medical University, Hefei 230032, Anhui,China;
²Department of Anesthesiology, the Second Affiliated Hospital of Anhui Medical University, Hefei 230601, Anhui, China

Received:2012-02-22 Revised:2012-04-26 Online:2012-05-26 Published:2012-05-28

摘要/Abstract

摘要： 目的:在原代培养的新生大鼠心肌细胞上观察低聚葡萄籽原花青素(oligomeric grape seed proanthocyanidins,GSP)对异丙肾上腺素(isoproterenol,Iso)诱导心肌细胞肥大的影响。方法: 采用SD大鼠乳鼠原代心肌细胞培养法,BCA法测定心肌细胞总蛋白含量;流式细胞仪检测心肌细胞内氧自由基(reactive oxygen species,ROS)含量;比色法检测丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性。结果: 1×10^-5 mol/L Iso能明显诱导心肌细胞肥大,GSP可剂量依赖性地抑制Iso诱导心肌细胞肥大,抑制细胞内ROS的生成,降低MDA含量、提高SOD活性。结论:GSP对Iso诱导的心肌细胞肥大具有明显的逆转作用, 其机制至少部分与抑制ROS生成、降低氧化应激水平有关。

关键词: 低聚葡萄籽原花青素, 异丙肾上腺素, 心肌细胞肥大, 氧自由基, 丙二醛, 超氧化物歧化酶

Abstract: AIM: To investigate the effect of oligomeric grape seed proanthocyanidins (GSP) on neonatal cardiomyocytes hypertrophy induced by isoproterenol (Iso).METHODS: Hypertrophy in cultured neonatal rat cardiac myocytes was induced by Iso. Total protein content was measured by BCA method; reactive oxygen species (ROS) were detected by flow cytometry; colorimetric method was employed to measured MDA content and SOD antivity.RESULTS:1×10^-5 mol/L Iso can activate β-AR completely and induce cardiomyocytes hypertrophy markedly. GSP therapy potently inhibited the increase of protein content, ROS generation and MDA content, inhibited the decrease of SOD activity with a dose-dependent manner.CONCLUSION: GSP possess protective effect against Iso induced cardiomyocytes hypertrophy, this may be related to reducing the ROS generation and oxidative stress level, at least in part.

Key words: Oligomeric grape seed proanthocyanidins, Isoproterenol, Cardiomyocytes hypertrophy, Reactive oxygen species, Malondialdehyde (MDA), Superoxide dismutase (SOD)

中图分类号:

R965.2

高杉, 黄春霞, 黄玲玲, 俞婷婷, 汪星辉, 张野. 低聚葡萄籽原花青素对异丙肾上腺素诱导心肌细胞肥大的影响[J]. 中国临床药理学与治疗学, 2012, 17(5): 497-501.

GAO Shan, HUANG Chun-xia, HUANG Ling-ling, YU Ting-ting, WANG Xing-hui, ZHANG Ye. Effect of oligomeric grape seed proanthocyanidins on prevention isoproterenol induced cardiomyocytes hypertrophy[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(5): 497-501.

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