芝麻素对自发性高血压大鼠肾病的保护作用

中国临床药理学与治疗学 ›› 2012, Vol. 17 ›› Issue (5): 502-507.

芝麻素对自发性高血压大鼠肾病的保护作用

杜少陵, 杨解人, 张俊秀, 李文星

皖南医学院国家中医药管理局中药药理三级实验室,芜湖 241002,安徽

收稿日期:2012-02-28 修回日期:2012-03-20 出版日期:2012-05-26 发布日期:2012-05-28
通讯作者: 杨解人,女,本科,教授,硕士生导师,研究方向:临床药理学。Tel: 0553-3932464 E-mail: wnmcyaoli@sina.com
作者简介:杜少陵,男,本科,副教授,研究方向:临床与分子药理。Tel: 0553-3932650 E-mail: wnmcdsl@yahoo.com.cn
基金资助:
安徽省教育厅自然科学研究基金重点项目(No2006KJ099A);芜湖市2008年度科技计划重点项目(No2008512)

Renal protective effect of sesamin in spontaneously hypertensive rats

DU Shao-ling, YANG Jie-ren, ZHANG Jun-xiu, LI Wen-xing

Third- Grade Pharmacology Laboratory of State Administration of Traditional Chinese Medicine,Wannan Medical College,Wuhu 241002,Anhui, China

Received:2012-02-28 Revised:2012-03-20 Online:2012-05-26 Published:2012-05-28

摘要/Abstract

摘要： 目的:观察芝麻素对自发性高血压大鼠(SHR)肾病的保护作用。方法: SHR灌胃芝麻素(160、80、40 mg/kg)16周,每日一次。测定动物给药前后血压及给药后血Cr和BUN含量;HE染色观察肾小球病理变化;MASSON 染色观察肾小球和肾间质胶原纤维变化;透射电镜观察肾小球超微结构。结果: 芝麻素(160 mg/kg)给药16周,能明显降低大鼠的血压、血Cr和BUN含量;改善肾小球变形、萎缩、硬化甚至玻璃样变等病理改变;肾小球系膜区未见明显扩大,基底膜未增厚,足突细胞无明显融合;肾小管细胞损伤和间质纤维化明显减轻。结论:芝麻素具有改善肾功能、保护肾脏作用。

关键词: 芝麻素, 自发性高血压大鼠, 肾脏, 病理学变化, 超微结构, 间质纤维化

Abstract: AIM: To evaluate the protective effect of sesamin on kidney damage in spontaneously hypertensive rats (SHR).METHODS: Sesamin(160,80,40 mg/kg)was administrated to SHR intragastrically once a day for 16 weeks. The blood pressure (BP) before and after administration were measured. After the last BP measuring, rats were anesthetized. 5 mL of blood sample were collected from abdominal aorta for the detecting of blood urea nitrogen (BUN) and creatinine(Cr) concentration. The kidney was excised for HE and MASSON staining to observe the changes of pathology and collagen fibers. Glomerular ultrastructure was observed by transmission electron microscopy.RESULTS:After the administration of sesamin for 16 weeks, the BP,Cr and BUN were lowered significantly. Pathological changes such as glomerular distortion, atrophy, sclerosis and hyalinization were improved. There was no significant expansion in mesangial area, no thickening in basement membrane and no significant cell fusion of podocytes. Renal tubular cell injury and interstitial fibrosis was significantly reduced.CONCLUSION: Sesamin can improve the renal function of SHR kidneys and protect kidneys from damage.

Key words: Sesamin, SHR, Kidney, Pathological changes, Glomerular ultrastructure, Interstitial fibrosis

中图分类号:

R965.2

杜少陵, 杨解人, 张俊秀, 李文星. 芝麻素对自发性高血压大鼠肾病的保护作用[J]. 中国临床药理学与治疗学, 2012, 17(5): 502-507.

DU Shao-ling, YANG Jie-ren, ZHANG Jun-xiu, LI Wen-xing. Renal protective effect of sesamin in spontaneously hypertensive rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(5): 502-507.

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