中国肾移植患者钙蛋白酶10基因多态性与移植后糖尿病的相关性研究

中国临床药理学与治疗学 ›› 2012, Vol. 17 ›› Issue (8): 901-905.

中国肾移植患者钙蛋白酶10基因多态性与移植后糖尿病的相关性研究

余爱荣¹, 范星², 刘慧明¹, 辛华雯¹

¹广州军区武汉总医院临床药理科, ²肾病内科,武汉 430070,湖北

收稿日期:2012-03-06 修回日期:2012-05-20 出版日期:2012-08-26 发布日期:2012-08-14
作者简介:余爱荣,女,博士,副主任药师,研究方向:临床药学。Tel: 027-68878692 E-mail: yarfwy@163.com
基金资助:
湖北省自然科学基金计划资助项目(2009CDB010)

Calpain10 gene polymorphisms are associated with posttransplantation diabetes mellitus in Chinese renal allograft recipients

YU Ai-rong¹, FAN Xing², LIU Hui-ming¹, XIN Hua-wen¹

¹Department of Clinical Pharmacology, ²Department of Nephrology, Wuhan General Hospital of Guangzhou Command, Wuhan 430070,Hubei,China

Received:2012-03-06 Revised:2012-05-20 Online:2012-08-26 Published:2012-08-14

摘要/Abstract

摘要： 目的: 探讨中国肾移植患者钙蛋白酶10(CAPN10)基因多态性与移植后糖尿病(PTDM)的相关性。方法: 采用等位基因特异性PCR、限制性片断长度多态性PCR(PCR-RFLP)分别检测了97例PTDM患者(PTDM组)和301例未发生PTDM的肾移植患者(对照组)的CAPN10基因SNP-19、SNP-43、SNP-63的基因型,采用 logistic 回归分析该基因多态性与PTDM的相关性。结果: PTDM组患者SNP-19的11 +12基因型频率和SNP-43的GG基因型频率明显高于对照组(P< 0.05)。用性别、移植时年龄、体重和BMI进行校正后, SNP-19 的11基因型和12基因型携带者移植术后发生PTDM的风险分别是22基因型的1.502倍(OR=1.502,95%CI:1.016~2.347,P=0.048)和1.764倍(OR=1.764,95%CI:1.055~2.947,P=0.030),SNP-43的GG基因型携带者移植术后发生PTDM的风险是AA和GA基因型患者的2.19倍(OR＝2.190,95%CI:1.047~3.473,P=0.044),SNP-63与PTDM的发生无明显相关性(P>0.05)。结论: CAPN10基因SNP-19 的1等位基因和SNP-43的GG基因型是肾移植后发生PTDM的独立危险因素。

关键词: 肾移植患者, 肾移植术后糖尿病, 危险因素, 钙蛋白酶10基因, 单核苷酸多态性

Abstract: AIM: To investigate the association between the Calpain10 gene polymorphism and the risk of PTDM in Chinese renal allograft recipients.METHODS: Three single nucleotide polymorphisms (CAPN10 gene SNP-19,SNP-43,SNP-63) were genotyped in the cohort, which consisted of 97 renal allograft recipients with PTDM (PTDM group) and 301 renal allograft recipients without PTDM (control group).The genotypes of polymorphisms were performed by allele specific polymerase chain reaction (ASPCR), PCR-restriction fragment length polymorphism (PCR-RFLP). Logistic regression test was used to identify risk factors for PTDM development and calculate the odds ratio.RESULTS: The 11 and 12 genotypes of SNP-19 and the GG genotypes of SNP-43 were more common in patients with PTDM than those without PTDM (P<0.05). After adjustments for age, sex, body weight and BMI, the effect of genotype remained significant (11 vs 22, OR=1.502, 95%CI: 1.016-2.347, P=0.048; 12 vs 22, OR=1.764, 95%CI: 1.055-2.947, P=0.030) in SNP-19 and the patients carrying genotype GG had higher risk comparison with carriers with genotype AA or GA(OR＝2.190, 95%CI: 1.047-3.473, P=0.044)in SNP-43. But SNP-63 was not association with PTDM.CONCLUSION: The 1-allele in SNP-19 and GG genotype in SNP-43 of CAPN10 gene are the independent risk factors of PTDM in Chinese renal allograft recipients.

Key words: Renal allograft recipients, Posttransplantation diabetes mellitus, Risk factor, Calpain10 gene, Single nucleotide polymorphism (SNP)

中图分类号:

R968

余爱荣, 范星, 刘慧明, 辛华雯. 中国肾移植患者钙蛋白酶10基因多态性与移植后糖尿病的相关性研究[J]. 中国临床药理学与治疗学, 2012, 17(8): 901-905.

YU Ai-rong, FAN Xing, LIU Hui-ming, XIN Hua-wen. Calpain10 gene polymorphisms are associated with posttransplantation diabetes mellitus in Chinese renal allograft recipients[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(8): 901-905.

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