4-羟苯维胺酯抑制大鼠膀胱癌的COX-2的表达和诱导肿瘤细胞凋亡的实验研究

中国临床药理学与治疗学 ›› 2012, Vol. 17 ›› Issue (9): 972-977.

4-羟苯维胺酯抑制大鼠膀胱癌的COX-2的表达和诱导肿瘤细胞凋亡的实验研究

沈群山, 黄后宝, 潘彩明, 薛珺, 蔡张愉, 王啸, 姜书传

皖南医学院弋矶山医院泌尿外科,芜湖 241001,安徽

收稿日期:2012-06-25 修回日期:2012-08-08 发布日期:2012-09-25
通讯作者: 黄后宝,通信作者,男,硕士,副教授,主任医师,硕士生导师,主要从事泌尿系统肿瘤方面研究。Tel: 0553-5739559 E-mail: drhuanghoubao@yahoo.com.cn
作者简介:沈群山,男,研究生,主要从事泌尿系统肿瘤方面研究。Tel: 0553-5739559 E-mail: sqsforever@yahoo.cn
基金资助:
安徽省教育厅临床医学应用技术科研课题(2006KJ136C)

Prevention effect of 4-HPR on bladder tumor in SD rats: An in vivo experiment

SHEN Qun-shan, HUANG Hou-bao, PAN Cai-ming, XUE Jun, CAI Zhang-yu, WANG Xiao, JIANG Shu-chuan

Department of Urology,Yijishan Hospital of Wannan Medical College,Wuhu 241001,Anhui,China

Received:2012-06-25 Revised:2012-08-08 Published:2012-09-25

摘要/Abstract

摘要： 目的使用N-甲基-N-亚硝基脲(N-methyl-N-nitrosourea,MNU)诱导SD大鼠膀胱肿瘤模型,观察4-羟苯维胺酯(4-HPR)的对大鼠膀胱肿瘤的干预效果。方法 70只SD大鼠随机分成对照组(A组)、造模组(B组)、造模处理组(C组、D组、E组),处理组分别予以4-HPR、阿霉素(ADM)、卡介苗(BCG)行膀胱内灌注。给药结束后1周处死大鼠,用免疫组化检测大鼠膀胱肿瘤中COX-2的表达和微血管密度(MVD) ;用TUNEL法检测凋亡。结果标本病理结果提示4-HPR、BCG、ADM对MNU诱导肿瘤的发生有明确的干预效果(P<0.05)。4-HPR能有效抑制COX-2的表达及微血管的生成并促使肿瘤细胞的凋亡(P<0.05)。结论 4-HPR、BCG、ADM对MNU的致癌过程均有干预作用,4-HPR与ADM、BCG的抗癌作用无显著性差异,且其副反应更少。

关键词: 膀胱肿瘤, 4-羟苯维胺酯, 卡介苗, 阿霉素

Abstract: AIM: To observe the curative effect of 4-HPR on bladder tumor using bladder tumor model induced by MNU in SD rats. METHODS: 70 SD rats was randomly divided into 5 groups: control group (Group A), module group (Group B), mould processing group (Group C, Group D, Group E). The bladder perfusion of treatment group was instilled 4-HPR, ADM and BCG respectively. A week after administration, the SD rats were put to death. The immunohistochemistry was used to detecte the expression of COX-2 and MVD, and the TUNEL technique was applied to examine apoptosis.RESULTS: The results of pathological specimens indicated that the 4-HPR, ADM and BCG inducing tumor had an obvious intervention effect (P<0.05); Besides, the 4-HPR could inhibit the expression of COX-2, the generation of capillaries, and the spur of apoptosis tumor cells (P<0.05).CONCLUSION: The 4-HPR, ADM and BCG to MNU had an intervention effect in carcinogenic process. The 4-HPR had no significant differences in anticancer effect compared with other groups, but had less side effect than the other groups.

Key words: Bladder tumor, 4-HPR, Bacillus Calmette-Guerin, Adriamycin

中图分类号:

R965.2

沈群山, 黄后宝, 潘彩明, 薛珺, 蔡张愉, 王啸, 姜书传. 4-羟苯维胺酯抑制大鼠膀胱癌的COX-2的表达和诱导肿瘤细胞凋亡的实验研究[J]. 中国临床药理学与治疗学, 2012, 17(9): 972-977.

SHEN Qun-shan, HUANG Hou-bao, PAN Cai-ming, XUE Jun, CAI Zhang-yu, WANG Xiao, JIANG Shu-chuan. Prevention effect of 4-HPR on bladder tumor in SD rats: An in vivo experiment[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(9): 972-977.

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