槲皮素对结肠癌LOVO细胞增殖侵袭能力及癌胚抗原CEA表达的影响

中国临床药理学与治疗学 ›› 2013, Vol. 18 ›› Issue (1): 24-29.

槲皮素对结肠癌LOVO细胞增殖侵袭能力及癌胚抗原CEA表达的影响

安昌勇¹, 谢刚¹, 汤为学², 杨小丁¹, 张才全¹

¹重庆医科大学附属第一医院胃肠外科,²重庆市神经病学重点实验室,重庆 400016

收稿日期:2012-09-20 修回日期:2012-09-20 发布日期:2013-02-05
通讯作者: 张才全,通信作者,男,教授、主任医师、博士生导师,主要从事胃肠道肿瘤基础与临床研究。 Tel: 13618393893 E-mail: zhangyu0323@21cn.com
作者简介:安昌勇, 男,硕士研究生,主要从事胃肠外科肿瘤疾病诊断与治疗方面的研究。 Tel: 13648372100 E-mail: 814112954@qq.com

Effect of Quercetin on proliferation and invasion of colon carcinoma LOVO cells and the expression level of carcinoembryonic antigen

AN Chang-yong¹, XIE Gang¹, TANG Wei-xue², YANG Xiao-ding¹, ZHANG Cai-quan¹

¹Department of Gastrointestinal Surgery, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China;
²Chongqing Key Laboratory of Neurology, Chongqing Medical University, Chongqing 400016, China

Received:2012-09-20 Revised:2012-09-20 Published:2013-02-05

摘要/Abstract

摘要： 目的: 研究槲皮素对结肠癌LOVO细胞增殖侵袭能力及癌胚抗原(CEA)表达的影响。方法: 采用MTT法检测不同浓度的槲皮素对LOVO细胞增殖能力的影响;内皮细胞粘附实验和小室侵袭实验检测槲皮素对LOVO细胞侵袭能力的影响;细胞免疫荧光、WB及RT-PCR检测槲皮素对LOVO细胞癌胚抗原CEA表达的影响。结果: 槲皮素能显著抑制LOVO细胞的增殖,呈浓度剂量依赖关系,IC₅₀约为 40 μmol/L;增强LOVO细胞与内皮细胞间的粘附能力,减弱其侵袭能力;并能显著抑制CEA蛋白及mRNA的表达水平,且呈浓度剂量依赖关系。结论: 槲皮素对LOVO细胞的增殖侵袭能力具有抑制作用,并能抑制CEA的表达。

关键词: 槲皮素, 结肠癌, 增殖, 侵袭, 癌胚抗原

Abstract: AIM: To investigate the effect of Quercetin on proliferation and invasion of colon carcinoma LOVO cells as well as the expression level of carcinoembryonic antigen. METHODS: LOVO cells were treated with Quercetin at various concentrations,then determined for proliferation level by MTT assay,for cell adhesion and invasion of LOVO cell by tumor cell adhere to the vascular endothelial cell assay and transwell chamber invasion assay, and for the expression level of CEA by Immunocellulerchemistry (ICC), Western Blot and RT-PCR.RESULTS: Significantly, Quercetin showed dose-dependent inhibitory effect on proliferation of LOVO cells with an IC₅₀ of about 40 μmol/L, also improved the ability of LOVO cells adhere to the vascular endothelial cells and reduced the invasion ability of LOVO cells. Meanwhile, Quercetin significantly inhibited the protein and mRNA expression levels of CEA in LOVO cells.CONCLUSION: Quercetin has inhibition effect on the proliferation invasion ability of LOVO cells,also inhibit the protein and mRNA expression levels of CEA in LOVO cells.

Key words: Quercetin, Colorectal cancer, Proliferation, Invasion, CEA

中图分类号:

安昌勇, 谢刚, 汤为学, 杨小丁, 张才全. 槲皮素对结肠癌LOVO细胞增殖侵袭能力及癌胚抗原CEA表达的影响[J]. 中国临床药理学与治疗学, 2013, 18(1): 24-29.

AN Chang-yong, XIE Gang, TANG Wei-xue, YANG Xiao-ding, ZHANG Cai-quan. Effect of Quercetin on proliferation and invasion of colon carcinoma LOVO cells and the expression level of carcinoembryonic antigen[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2013, 18(1): 24-29.

参考文献

[1] Murakami A.Mutitargeted cancer prevention by quercetin[J]. Cancer Lett, 2008, 269(2): 315-325.
[2] 王刚,杜士明,杨光义.槲皮素抗肿瘤的分子机制研究进展[J].中国医院药学杂志, 2011, 31(4): 322-324.
[3] Kelly GS.Quercetin[J]. Altern Med Rev,2011,16(2):172-194.
[4] Zhou JQ, Tang ZQ.Effect of quercetin on CYP1A2,CYP2E1,CYP3A2 activities and its inhibitory mechanism studies in rat liver microsomes[J]. J Chin Pham Sci, 2005,14(4):231-236.
[5] 易飞,周宏灏.天然药对CYP3A的影响[J].中国临床药理与治疗学, 2008,13(3): 344-349.
[6] Cruz-Correa M, Shoskes DA, Sanchez P, et al.Combination treatment with curcumin and quercetin of adenomas in familial adenomatous polyposis[J]. Clin Gastroenterol Hepatol, 2006, 4(8): 1035-1038.
[7] Djuric Z, Severson RK, Kato I.Association of dietary quercetin with reduced risk of proximal colon cancer[J]. Nutr Cancer, 2012, 64(3): 351-360.
[8] Zhang H, Zhang M, Yu L.Antitumor activities of quercetin and quercetin-5',8-disulfonate in human colon and breast cancer cell lines[J]. Food Chem Toxicol, 2012, 50(5):1589-1599.
[9] Duxbury MS, Ito H, Zinner MJ, et al.CEACAM6 gene silencing impairs anoikis resistance and in vivo metastatic ability of pancreatic adenocarcinoma cells[J]. Oncogene, 2004, 23(2):465-473.
[10] Taheri M, Saragovi HU, Stanners CP.The adhesion and differentiation-inhibitory activities of the immunoglobulin superfamily member, carcinoembryonic antigen, can be independently blocked[J]. J Biol Chem, 2003, 278(17): 14632-14639.
[11] Han SU, Kwak TH, Her KH, et al.CEACAM5 and CEACAM6 are major target genes for Smad3-mediated TGF-β signaling[J]. Oncogene, 2008, 27(5): 675-683.
[12] Yasuda S, Kamata H, Machida T, et al.A case of isolated paraaortic lymph node recurrence from colon cancer successfully treated with chemoradiotherapy[J]. Tokai J Exp Clin Med, 2012, 37(2): 47-50.
[13] Cohen S, Margel S.Engineering of near IR fluorescent albumin nanoparticles for in vivo detection of colon cancer[J]. J Nanobiotechnology, 2012, 10(1): 36.
[14] Li Y, Cao H, Jiao Z, et al.Carcinoembryonic antigen interacts with TGF-β receptor and inhibits TGF-β signaling in colorectal cancers[J]. Cancer Res, 2010, 70(20): 8159-8168.
[15] Bast RC Jr, Ravdin P, Hayes DF, et al.2000 update of recommendations for the use of tumor markers in breast and colorectal cancer: clinical practice guidelines of the American Society of Clinical Oncology[J]. Clin Oncol, 2001,19(6):1865-1878.
[16] Blumenthal RD, Osorio L, Hayes MK, et al.Carcinoembryonic antigen antibody inhibits lung metastasis and augments chemotherapy in a human colonic carcinoma xenograft[J]. Cancer Immunol Immunother, 2005, 54(4): 315-327.