丹参注射液对小鼠心肌干细胞的影响

中国临床药理学与治疗学 ›› 2013, Vol. 18 ›› Issue (10): 1105-1109.

丹参注射液对小鼠心肌干细胞的影响

余洪松¹, 凌琳², 姜宏³, 施广飞²

¹南京中医药大学中西医结合临床医学院心内科,南京 210008,江苏;
²南京大学医学院附属鼓楼医院心内科,南京 210008,江苏;
³南京中医药大学第一临床医学院诊断教研室,南京 210046,江苏

收稿日期:2012-08-06 修回日期:2013-03-20 出版日期:2013-10-26 发布日期:2013-09-30
通讯作者: 施广飞,男,硕士,主任医师,教授,博士生导师,研究方向:心脏电生理。Tel: 025-83304616-20400 E-mail: shi_guangfei@126.com
作者简介:余洪松,男,硕士,副主任医师,研究方向:心脏干细胞及电生理。Tel: 025-83304616-20400 E-mail: yuhonson@126.com

Effects of salviae miltiorrhizae on mice resident cardiac stem cell

YU Hong-song¹, LING Lin², JIANG Hong³, SHI Guang-fei²

¹Department of Cardiology, Integrated Traditional and Western Medicine Clinical College of Nanjing University of Traditional Chinese Medicine, Nanjing 210008, Jiangsu, China;
²Department of Cardiology, Affiliated Gulou Hospital of Nanjing University Medical School, Nanjing 210008, Jiangsu, China;
³First Clinical College of Nanjing University of Chinese Medicine, Nanjing 210046, Jiangsu, China

Received:2012-08-06 Revised:2013-03-20 Online:2013-10-26 Published:2013-09-30

摘要/Abstract

摘要： 目的:探讨丹参注射液对小鼠原位心脏成体干细胞数量和旁分泌功能的影响。方法:以不同剂量丹参注射液对小鼠进行灌胃给药,分为高剂量丹参注射液组(5 g·kg^-1·d^-1)和低剂量丹参注射液组(0.5 g·kg^-1·d^-1),对照组采用相同体积生理盐水,每天1次连续1周。1周后经小鼠眼眶取血,检测血常规及肝肾功能;以小鼠新鲜心脏组织冰冻切片行免疫荧光染色,检测Sca-1⁺成体干细胞数量;以Real-Time PCR检测心脏血管内皮生长因子(VEGF)、IL-6、BAD基因表达情况。结果:灌胃1周后,三组间血常规、肝肾功能差异无统计学意义(P>0.05);与高剂量丹参注射液组比较,低剂量丹参注射液及对照组中Sca-1⁺成体干细胞数量及VEGF和IL-6基因表达显著减少,差异具有统计学意义(P<0.05)。同时,丹参注射液高剂量组BAD基因表达较丹参注射液低剂量组和对照组显著降低,差异具有统计学意义(P<0.05)。结论:丹参注射液能增多小鼠心脏中成体心脏干细胞数量,改善旁分泌功能。

关键词: 丹参, 心脏成体干细胞, 旁分泌功能

Abstract: AIM: To investigate the effect of Salviae miltiorrhizae on the number and paracrine effect of resident cardiac stem cell.METHODS: Mice were divided into three groups and received intragastric administration of high dose Salviae miltiorrhizae (5 g·kg^-1·d^-1), low dose Salviae miltiorrhizae (0.5 g·kg^-1·d^-1) or saline for one week. Blood was collected for analysis of blood routin test, liver and renal function. The number of resident Sca-1⁺ cardiac stem cells was detected by immunofluorescent staining and the expressions of VEGF, IL-6 and BAD in heart tissue were determined with Real-Time PCR .RESULTS: The number of white blood cell, red blood cell, platelet, liver and renal function showed no difference among the three groups (P>0.05),by intragastric administration for 1 week.Compared with the high dose Salviae miltiorrhizae group,the number of resident Sca-1⁺ cardiac stem cells and the expression of VEGF, IL-6 were decreased in low dose Salviae miltiorrhizae group and control group,there was statistically significant difference (P<0.05). Meanwhile, compared the other two groups ,the expression of BAD was decreased in the high dose Salviae miltiorrhizae group,there was statistically significant difference (P<0.05).CONCLUSION: Salviae miltiorrhizae treatment can increase resident Sca-1⁺ cardiac stem cells number and improve paracrine function.

Key words: Salviae miltiorrhizae, Cardiac stem cell, Paracrine function

中图分类号:

R965.2

余洪松, 凌琳, 姜宏, 施广飞. 丹参注射液对小鼠心肌干细胞的影响[J]. 中国临床药理学与治疗学, 2013, 18(10): 1105-1109.

YU Hong-song, LING Lin, JIANG Hong, SHI Guang-fei. Effects of salviae miltiorrhizae on mice resident cardiac stem cell[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2013, 18(10): 1105-1109.

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