季德胜蛇药片对大鼠肺缺血再灌注损伤的保护作用

中国临床药理学与治疗学 ›› 2013, Vol. 18 ›› Issue (11): 1219-1223.

季德胜蛇药片对大鼠肺缺血再灌注损伤的保护作用

陶冬英, 倪晶晶, 任典寰, 于纪棉, 张秀娟, 万勇

浙江宁波卫生职业技术学院人体形态教研室,宁波 315104,浙江

收稿日期:2013-07-22 修回日期:2013-10-21 出版日期:2013-11-26 发布日期:2013-11-22
作者简介:陶冬英,女,硕士研究生,研究方向:基质金属蛋白酶及其抑制剂方面的研究。Tel: 0574-88126264 E-mail: lotus0903@126.com
基金资助:
浙江省教育厅课题项目(Y201226089)

The protective effects of Jidesheng Sheyao pian on lung ischemia-reperfusion injury in rats

TAO Dong-ying, NI Jing-jing, REN Dian-huan, YU Ji-mian, ZHANG Xiu-juan, WANG Yong

Department of Human Morphology, Ningbo College of Health Science, Ningbo 315104, Zhejiang, China

Received:2013-07-22 Revised:2013-10-21 Online:2013-11-26 Published:2013-11-22

摘要/Abstract

摘要： 目的: 探讨季德胜蛇药片对大鼠肺缺血再灌注损伤的保护作用及其机制。方法: 将30只清洁级SD雄性大鼠分为季德胜蛇药组(D组)、对照组(C组)和缺血再灌注组(I/R组),每组10只。建立大鼠肺缺血再灌注模型,检测大鼠肺组织湿干重比;光学显微镜及透射电镜下观察肺组织损伤程度;通过明胶酶谱法测定肺组织匀浆液中明胶酶即金属蛋白酶-2、-9(MMP-2、MMP-9)的活力;逆转录酶PCR方法检测MMP-2、MMP-9 mRNA在大鼠肺组织中的表达变化。结果: 与缺血再灌注组比较,蛇药组肺湿干重比降低(P<0.01),肺组织损伤程度减轻,肺组织匀浆液MMP-9酶活力显著下降,MMP-2酶活力略有降低,肺组织中MMP-9、MMP-2的mRNA表达均降低。 结论: 季德胜蛇药片通过抑制MMP-9、MMP-2的活性及表达,使基底膜的降解降低,从而对大鼠肺缺血再灌注损伤有一定的保护作用。

关键词: 季德胜蛇药片, 肺缺血-再灌注损伤, 基质金属蛋白酶

Abstract: AIM: To investigate the protective effects and the mechanisms of Jidesheng Sheyao pian on lung ischemia-reperfusion injury of rats.METHODS: Thirty SD male rats were divided into Jidesheng Sheyao group (n=10), control group (n=10) and ischemia-reperfusion injury group (I/R, n=10). The models of lung ischemia-reperfusion injury of SD rats were established. Then, the wet/dry ratio was measured, the histopathology of the lung injury was observed by light microscope and electron microscope, the activity of matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9 (MMP-9) in the lung homogenates were detected by zymography, RT-PCR the expression of MMP-2, MMP-9 mRNA were detected in rat lung tissues.RESULTS: Compared with the I/R group, the wet/dry ratio were significantly decreased (P<0.01), the degree of lung injury showed lighter, the activity of MMP-9 in lung homogenate presented inhibition (P<0.01) in the drug group and the expression of MMP-9, MMP-2 mRNA was also depressed than that of the I/R group.CONCLUSION: Jidesheng Sheyao pian can inhibit the expression and activity of MMP-9 and MMP-2, attenuate basement membrane degradation, sequentially, has protective effects on lung ischemia-reperfusion injury in rats.

Key words: Jidesheng Sheyao pian, Lung ischemia-reperfusion injury, Matrix metalloproteinase

中图分类号:

陶冬英, 倪晶晶, 任典寰, 于纪棉, 张秀娟, 万勇. 季德胜蛇药片对大鼠肺缺血再灌注损伤的保护作用[J]. 中国临床药理学与治疗学, 2013, 18(11): 1219-1223.

TAO Dong-ying, NI Jing-jing, REN Dian-huan, YU Ji-mian, ZHANG Xiu-juan, WANG Yong. The protective effects of Jidesheng Sheyao pian on lung ischemia-reperfusion injury in rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2013, 18(11): 1219-1223.

参考文献

[1] Yano M, Omoto Y, Yamakawa Y, et al. Increased matrix metalloproteinase 9 activity and mRNA expression in lung ischemia-reperfusion injury[J]. Heart Lung Transplant, 2001, 20(6): 679-686.
[2] Yeh DY, Lin HI, Feng NH, et al. Matrix metalloprotease expressions in both reperfusion lung injury and oleic acid lung injury models and the protective effects of ilomastat[J]. Transplant Proc, 2009, 41(5): 1508-1511.
[3] Shimoyama T, Tabuchi N, Chung J, et al. Matrix metalloproteinase inhibitor (ONO-4817) attenuates ischemia-reperfusion injury in rat lung [J]. Med Sci Monit, 2006, 12(2): BR51- BR56.
[4] Sommer SP, Sommer S, Sinha B, et al. Ischemia-reperfusion injury-induced pulmonary mitochondrial damage [J]. Heart Lung Transplant, 2011, 30(7): 811-818.
[5] Dejonckheere E, Vandenbroucke RE, Libert C. Matrix metalloproteinases as drug targets in ischemia/ reperfusion injury[J]. Drug Discov Today, 2011, 16(17/18): 762-778.
[6] 王莉. 基质金属蛋白酶抑制剂的筛选的研究[D]. 南京: 东南大学基础医学院.
[7] 陶冬英, 陈平圣, 刘东风. 季德胜蛇药片对大鼠急性肺损伤的保护作用[J]. 中华急诊医学杂志, 2006, 15(3): 223-227.
[8] Eppinger MJ, Deeb GM, Bolling SF, et al. Mediators of ischemia-reperfusion injury of rat lung[J]. Am J Pathol, 1997, 150(5): 1773-1784.
[9] 陶冬英. 季德胜蛇药片对大鼠ALI/ARDS的预防作用研究[D]. 南京: 东南大学基础医学院.
[10] 司裕龙, 张文凯. 重组人白介素-10对大鼠肺缺血再灌注损伤的保护作用[J]. 中国临床药理学与治疗学, 2011, 16(11): 1239-1243.
[11] 贾伟伟, 李慧. 依达拉奉预处理对兔肺缺血再灌注损伤后肺水肿的影响[J]. 中国现代医生, 2013, 51(1): 1-7.
[12] Torii K, Iida K, Miyazaki Y, et al. Higher concentrations of matrix metalloproteinases in bronchoalveolar lavage fluid of patients with adult respiratory distress syndrome[J]. Am J Respir Crit Care Med, 1997, 155(1): 43-46.
[13] 丁丽娟, 汤伟, 汪晓, 等. 季德胜蛇药对大鼠急性肝损伤的保护作用及机制的初步研究[J]. 交通医学, 2009, 23(1): 5-8.
[14] 刘永平, 曾治平. 多西环素减轻小鼠内毒素性急性肺损伤的作用及机制研究[J]. 中国临床药理学与治疗学, 2009, 14(10): 1133-1136.
[15] 罗海燕, 孙宗全, 蒋雄刚, 等. 基质金属蛋白酶抑制剂对肺缺血再灌注损伤的保护作用[J]. 华中科技大学学报:医学版, 2007, 36 (2): 206-209.

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