芝麻素对肾性高血压伴高血脂大鼠主动脉舒张功能的影响

中国临床药理学与治疗学 ›› 2013, Vol. 18 ›› Issue (4): 366-370.

芝麻素对肾性高血压伴高血脂大鼠主动脉舒张功能的影响

孔祥, 杨解人, 郭莉群, 周勇

皖南医学院药理学教研室,国家中医药管理局中药药理三级实验室,芜湖 241002,安徽

收稿日期:2012-10-30 出版日期:2013-04-26 发布日期:2013-04-26
通讯作者: 杨解人,女,教授,硕士生导师,研究方向:心血管药理。Tel: 0553-3932464 E-mail: wnmcyaoli@sina.com
作者简介:孔祥,男,硕士,讲师,研究方向:心血管及内分泌药理。E-mail: wnmcyaolikx@sina.com
基金资助:
安徽省教育厅自然科学研究基金重点项目(2006KJ099A);安徽省自然科学基金项目(1308085QH145);皖南医学院中青年科研基金项目(WK201206)

Effects of sesamin on aortic relaxation in renal hypertensive-hyperlipidemia rats

KONG Xiang, YANG Jie-ren, GUO Li-qun, ZHOU Yong

Department of Pharmacology, Third-Grade Pharmacology Laboratory of State Administration of Traditional Chinese Medicine, Wannan Medical College, Wuhu 241002, Anhui, China

Received:2012-10-30 Online:2013-04-26 Published:2013-04-26

摘要/Abstract

摘要： 目的: 探讨芝麻素(Sesamin,Ses)改善肾性高血压伴高血脂大鼠(Rrenal hypertensive-hyperlipidemia rat,RHHR)主动脉舒张功能损伤的作用机制。方法: RHHR灌服不同剂量Ses(100、33、10 mg·kg^-1·d^-1)8周后,测定主动脉环对乙酰胆碱(Acetylcholine,ACh)和硝普钠(Sodium nitroprusside,SNP)的舒张反应;测定一氧化氮合酶抑制剂左旋硝基精氨酸甲酯(N-nitro-L-arginine-methyl-ester,L-NAME)孵育后,动脉环对ACh的反应并计算一氧化氮(Nitric oxide,NO)活性。结果: 与假手术组相比,RHHR主动脉Ach和SNP诱导的舒张反应显著降低,NO活性减少,Ses治疗8周后能逆转上述作用。结论: Ses降压作用与改善RHHR主动脉内皮依赖性和非内皮依赖性舒张功能损伤有关。

关键词: 芝麻素, 肾性高血压伴高血脂大鼠, 内皮依赖性舒张, 非内皮依赖性舒张

Abstract: AIM: To investigate the possible in vivo protective effects of sesamin on aortic relaxation in renal hypertensive-hyperlipidemia rats (RHHR).METHODS: RHHR were orally administrated with sesamin at a dose of 100, 33 and 10 mg·kg^-1·d^-1, respectively. After 8 weeks of treatment, the vasorelaxation response to acetylcholine (ACh) and sodium nitroprusside (SNP) was measured in aortic rings. Furthermore, the aortic response to ACh after being incubated with N-nitro-L-arginine-methyl-ester and NO bioactivity were determined.RESULTS: Compared with the sham-operated rats, ACh- and SNP- induced aortic vasorelaxation and NO bioactivity were obviously decreased in RHHR. These abnormalities were essentially reversed by treatment with sesamin for 8 weeks.CONCLUSION: All these results suggest that chronic treatment with sesamin reduces hypertension through improvement of impaired endothelium-dependent and -independent vasorelaxation in RHHR.

Key words: Sesamin, Renal hypertensive-hyperlipidemia rat, Endothelium-dependent relaxation, Endothelium-independent relaxation

中图分类号:

R965.1

孔祥, 杨解人, 郭莉群, 周勇. 芝麻素对肾性高血压伴高血脂大鼠主动脉舒张功能的影响[J]. 中国临床药理学与治疗学, 2013, 18(4): 366-370.

KONG Xiang, YANG Jie-ren, GUO Li-qun, ZHOU Yong. Effects of sesamin on aortic relaxation in renal hypertensive-hyperlipidemia rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2013, 18(4): 366-370.

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