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中国临床药理学与治疗学 ›› 2013, Vol. 18 ›› Issue (5): 532-536.

• 临床药理学 • 上一篇    下一篇

高效液相色谱法定量尿酸、黄嘌呤和次黄嘌呤在人体血清中的浓度

施政1, 刘健2, 申屠建中2, 王建平1   

  1. 1浙江省中医院药剂科,杭州 310006,浙江;
    2浙江大学医学院附属第一医院临床药学研究中心,杭州 310003,浙江
  • 收稿日期:2012-10-25 修回日期:2013-03-06 出版日期:2013-05-26 发布日期:2013-05-22
  • 通讯作者: 申屠建中,男,副主任药师,硕士生导师,研究方向:临床药理药动学及基础药理。Tel: 0571-87236560 E-mail: stjz@zju.edu.cn
  • 作者简介:施政,主管药师,研究方向:临床药理学。Tel: 13857188289 E-mail: frysz@163.com

Quantification of uric acid, xanthine and hypoxanthine in human serum by HPLC

SHI Zheng1 , LIU Jian2, SHENTU Jian-zhong2, WANG Jian-ping1   

  1. 1Department of Pharmacy, Traditional Chinese Medicine Hospital of Zhejiang, Hangzhou 310006, Zhejiang,China;
    2Research Center for Clinical Pharmacy, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, Zhejiang,China
  • Received:2012-10-25 Revised:2013-03-06 Online:2013-05-26 Published:2013-05-22

摘要: 目的: 建立高效液相色谱法测定尿酸、黄嘌呤和次黄嘌呤在人体血清中的浓度。方法: 用Agilent ZORBAX SB-Aq column色谱柱,ZORBAX-Extend C18预柱,以甲醇和 47 mmol/L KH2PO4水溶液作为流动相,流速:1.0 mL/min,检测波长为 260 nm。结果: 血清中尿酸、黄嘌呤和次黄嘌呤的线性范围分别是(1.667~166.667)×103 ng/mL (r=0.9995)、(0.033~3.338)×103 ng/mL(r=0.9994)和 (0.033~3.338)×103 ng/mL (r=0.9996),日间与日内精密度均<15%。结论: 该法灵敏度高,精密度和准确度佳,能满足非布司他体内药效学研究。

关键词: 人体血清, 尿酸, 黄嘌呤, 次黄嘌呤, HPLC-UV, 非布司他

Abstract: AIM: To develop a specific, sensitive, and accurate HPLC method to measure uric acid, xanthine and hypoxanthine concentrations in human serum.METHODS: Agilent ZORBAX SB-Aq column coupled with a ZORBAX-Extend C18 guard column was employed. The mobile phase consisted of methanol/ 47 mmol/L KH2PO4 solution and the flow rate was 1.0 mL/min. The detection wavelength was 260 nm.RESULTS: Good linearity of uric acid, xanthine, hypoxanthine in human serum were (1.667-166.667)×103 ng/mL(r=0.9995), (0.033-3.338)×103 ng/mL(r=0.9994), (0.033-3.338)×103 ng/mL (r=0.9996) , respectively. Intra- and inter-day validation were both less than 15%.CONCLUSION: A rapid, accurate and specific method was developed and validated for the determination of uric acid, xanthine and hypoxanthine in human serum. It was suitable for the pharmacodynamic studies of febuxostat.

Key words: Human serum, Uric acid, Xanthine, Hypoxanthine, HPLC-UV, Febuxostat

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