不同内径银夹诱导2K1C高血压模型的比较

中国临床药理学与治疗学 ›› 2013, Vol. 18 ›› Issue (6): 621-626.

不同内径银夹诱导2K1C高血压模型的比较

黄玲玲¹, 俞婷婷¹, 郭焜¹, 兰潮棕², 刘彪¹, 宋佳治¹, 刘有静¹, 汪星辉¹, 高杉¹

¹安徽医科大学基础医学院药理学教研室,合肥 230032,安徽,
²安徽中医药大学,合肥 230031,安徽

收稿日期:2013-01-14 修回日期:2013-03-26 发布日期:2013-06-19
通讯作者: 高杉,男,教授,硕士生导师,研究方向:心血管药理学。Tel: 13083495662 E-mail: aydgs@126.com
作者简介:黄玲玲,女,硕士,研究方向:心血管药理学。Tel: 13615698660 E-mail: vivianhll@sina.com
基金资助:
国家自然科学基金资助项目(81073088);教育部高等学校博士学科点专项科研基金资助项目(20103420120002);安徽医科大学中青年学术技术带头人资助

Comparison of different inner diameter silver clip impact on 2K1C hypertension model

HUANG Ling-ling¹, YU Ting-ting¹, GUO Kun¹, LAN Chao-zong², LIU Biao¹, SONG Jia-zhi¹, LIU You-jing¹, WANG Xing-hui¹, GAO Shan¹

¹Department of Pharmacology, College of Basic Medicine, Anhui Medical University, Hefei 230032, Anhui, China,
²College of Pharmacy, Anhui Uninersity of Traditional Chinese Medicine, Hefei 230031, Anhui, China

Received:2013-01-14 Revised:2013-03-26 Published:2013-06-19

摘要/Abstract

摘要： 目的: 比较不同内径银夹缩窄大鼠肾动脉诱导的2K1C高血压模型效果。方法: 用U型银夹缩窄大鼠右侧肾动脉建立2K1C高血压模型,分为4组:假手术组、2K1C-0.2 mm 组、2K1C-0.25 mm 组和2K1C-0.3 mm 组。0~8周内,用无创尾动脉压测量仪测量大鼠尾动脉压。第8周末,BL-420S生物机能实验系统监测各组动物的心功能,计算大鼠心重量指数(HW/BW)、左和右肾脏重量指数(LKW/BW和RKW/BW),HE染色观察胸主动脉重构情况,比色法测定心肌组织羟脯氨酸含量,放免法测定血清中血管紧张素II(Ang II)的含量。结果: 与假手术组比较,2K1C-0.25 mm 组和2K1C-0.3 mm 组,成模率高、肾脏固缩少,心功能损伤明显,HW/BW、羟脯氨酸和Ang II的含量明显升高,胸主动脉发生明显重构;而2K1C-0.2 mm 组,成模率低、肾脏固缩多,心功能损伤不明显。结论: 0.25 mm 和 0.3 mm 内径的银夹诱导的2K1C肾性高血压模型,成模率高,模型更稳定。

关键词: 高血压, 肾素血管紧张素系统, 银夹内径, 2K1C高血压模型

Abstract: AIM: To compare the effect of three different inner diameter silver clips on 2K1C hypertensive rats.METHODS: A 2K1C hypertensive rats were established by narrow the right renal artery with different inner diameter of U-shaped silver clips. Animals were divided into 4 groups: the sham-operate group, 2K1C-0.2 mm group, 2K1C-0.25 mm group and 2K1C-0.3 mm group. The tail-cuff apparatus was employed to measure the rat tail arterial blood pressure during the 0-8 weeks. At the end of 8^th wk, the hemodynamics index recorded. The cardiac and kidney hypertrophy index were expressed as heart weight/body weight (HW/BW), left kidney weight/body weight (LKW/BW) and right kidney weight/body weight (RKW/BW). The aorta remodeling was investigated by HE stain. The content of hydroxyproline in myocardial tissue was measured by colorimetric determination. Radioimmunoassay method was used to detected the AngII content in serum.RESULTS: The 2K1C-0.25 group and 2K1C-0.3 group showed the higher model successful rate, less pyknotic kidneys, significant impairment of cardiac function, markedly increasing of HW/BW, vascular remodeling index, the content of hydroxyproline and the AngII content compared with sham-operate group. However, the 2K1C-0.2 group performed not well in model successful rate, pyknotic kidneys and the AngII content in serum.CONCLUSION: The 2K1C renovascular hypertension model that narrow the renal artery with 0.25 or 0.3 mm inner diameter of U-shaped silver clips proves to be the optimal one.

Key words: Hypertension, Renin-angiotensin system, Inner diameter of silver clip, 2K1C renovascular hypertension model

中图分类号:

R965.1

黄玲玲, 俞婷婷, 郭焜, 兰潮棕, 刘彪, 宋佳治, 刘有静, 汪星辉, 高杉. 不同内径银夹诱导2K1C高血压模型的比较[J]. 中国临床药理学与治疗学, 2013, 18(6): 621-626.

HUANG Ling-ling, YU Ting-ting, GUO Kun, LAN Chao-zong, LIU Biao, SONG Jia-zhi, LIU You-jing, WANG Xing-hui, GAO Shan. Comparison of different inner diameter silver clip impact on 2K1C hypertension model[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2013, 18(6): 621-626.

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