自发性高血压大鼠左室心肌壁内小动脉平滑肌瞬时受体电位通道的表达

中国临床药理学与治疗学 ›› 2013, Vol. 18 ›› Issue (8): 879-884.

自发性高血压大鼠左室心肌壁内小动脉平滑肌瞬时受体电位通道的表达

薛婉婉^1,2, 洪华山¹, 林晓红¹, 林军华¹, 方美琴¹

¹福建医科大学附属协和医院干部病房, 福州 350001,福建;
²厦门大学附属第一医院干部病房, 厦门 361000,福建

收稿日期:2012-11-07 修回日期:2013-04-11 出版日期:2013-08-26 发布日期:2013-07-31
通讯作者: 洪华山,男,博士,教授,博士生导师,研究方向:心血管基础与临床。Tel: 15959159898 E-mail: honghuashan@medmail.com.cn
作者简介:薛婉婉,女,硕士研究生,研究方向:心血管病学。Tel: 15860751863 E-mail: 1150122159@qq.com.cn
基金资助:
国家自然科学基金项目(81170143); 福建医科大学重大课题资助项目(09ZD010)

Expression of TRPC in the smooth muscle cells of the small intramyocardial arteries in the left ventricles of spontaneously hypertensive rats

XUE Wan-wan^1,2, HONG Hua-shan¹, LIN Xiao-hong¹, LIN Jun-hua¹, FANG Mei-qin¹

¹Fujian Medical University Union Hospital, Cadres Ward,Fuzhou 350001, Fujian, China;
²The First Hospital Affiliated to Xiamen University, Cadres Ward, Xiamen 361000, Fujian, China

Received:2012-11-07 Revised:2013-04-11 Online:2013-08-26 Published:2013-07-31

摘要/Abstract

摘要： 目的: 探索自发性高血压大鼠(Spontaneously hypertensive rats,SHR)心肌壁内小动脉重构与瞬时受体电位通道(Transient receptor potential channel,TRPC)蛋白表达的相关性。方法: 24周龄雄性SHR(n=8)为实验组,设同周龄同性别正常血压大鼠为对照组(WKY,n=8)。计算大鼠左室质量指数(Left ventficular mass index,LVMI)评价左室肥厚情况。采用HE染色观察管腔直径为10～69 μm 的左室心肌壁内小动脉结构特点并测定管腔面积(Lumen area,LA)、管壁面积(Wall area,WA)、管壁面积/管腔面积(WA／LA)。α-平滑肌肌动蛋白(Alpha-smooth muscle actin,α-SMA)免疫荧光染色鉴定血管平滑肌细胞(Vascular smooth muscle cell,VSMC)。免疫荧光染色检测平滑肌细胞TRPC1、TRPC3、TRPC5、TRPC6蛋白表达强度。结果: SHR组SBP及LVMI均明显高于WKY组[(200±4) vs (118±9) mm Hg,1 mm Hg=0.133 kPa;(3.11±0.03) vs(2.42±0.10) mg/g,均P<0.05];SHR组WA及WA／LA均比WKY组增加[(2951±224) vs (2654±190) μm²;(2.01±0.04) vs(1.53±0.03),均P<0.05],而LA比WKY组减少[(1469±117) vs(1730±98) μm²,P<0.05],α-SMA免疫荧光染色阳性表明增厚的血管中层为平滑肌细胞。SHR组心肌壁内小动脉平滑肌细胞TRPC1 、TRPC3蛋白表达均明显高于WKY组(分别为 65±8 vs 28±3和60±4 vs 26±8,均P<0.05),TRPC5蛋白表达与WKY组无统计学差异(28±7 vs 28±7,P>0.05);SHR 组与WKY组均未表达TRPC6。结论: 24周SHR左室心肌壁内小动脉平滑肌表达TRPC1、TRPC3、TRPC5而未表达TRPC6。24周SHR左室心肌壁内小动脉重构与平滑肌TRPC1、TRPC3表达上调相关。

关键词: 心肌壁内小动脉, 血管重构, 血管平滑肌细胞, 瞬时受体电位通道, 自发性高血压大鼠

Abstract: AIM: To investigate the relationship between the remodelling of small intramyocardial arteries(SIMAs) and the expression of transient receptor potential channel (TRPC) in spontaneously hypertensive rats (SHR).METHODS: 24-weeks-old male of SHR taken as the experimental group (SHR group,n=8 ), and the wistar-kyoto rats (WKY,n=8) with matching age and male systolic blood pressure (SBP) is normal as the control group. Left ventriclar mass index (LVMI) was calculated to evaluate the hypertrophy degree of the left ventricle. The structure feature of the small intramyocardial arteries with diameters from 10 to 69 μm in the left ventricles was evaluated with HE staining.The wall area (WA), lumen area (LA), wall area/lumen area (WA/LA) of SIMAs were measured. Vascular smooth muscle cells (VSMCs) were recognized with alpha-smooth muscle actin (α-SMA) immunofluorescence staining.The expression of TRPC1, TRPC3, TRPC5, TRPC6 were also detected with immunofluorescence staining.RESULTS: Both SBP and LVMI of the SHR group were significantly higher than those in WKY group 〔(200±4) vs (118±9) mm Hg;(3.11±0.03) vs (2.42±0.10) mg/g respectively,both P<0.05〕. Both WA and WA/LA of the SHR group were larger than those in WKY group〔(2951±224) vs (2654±190) μm²;(2.01±0.04) vs (1.53±0.03),both P﹤0.05 〕. However, LA of the SHR group was smaller than that of WKY group〔(1469±117) vs (1730±98) μm² ,P<0.05〕. Positive α-SMA immunofluorescence staining indicated that the thickening middle layer of the SIMAs was smooth muscle cells. The expressions of TRPC1 and TRPC3 on the SIMAs of SHR group were significantly higher than those in WKY group(65±8 vs 28±3 and 60±4 vs 26±8 respectively,both P<0.05). The expressions of TRPC5 in two groups were not statistically different(28±7 vs 28±7,P>0.05). TRPC6 was not expressed on the SIMAs in both groups.CONCLUSION: The smooth muscle cells of the small intramyocardial arteries in 24-weeks-old SHR expresses TRPC1,TRPC3 and TRPC5 but not TRPC6. The remodelling of the small intramyocardial arteries in SHR is associated with the up-regulation of TRPC1 and TRPC3.

Key words: Small intramyocardial arteries, Vascular remodeling, Vascular Smooth muscle cell, Transient receptor potential channel, Spontaneously hypertensive rats

中图分类号:

R965.2

薛婉婉, 洪华山, 林晓红, 林军华, 方美琴. 自发性高血压大鼠左室心肌壁内小动脉平滑肌瞬时受体电位通道的表达[J]. 中国临床药理学与治疗学, 2013, 18(8): 879-884.

XUE Wan-wan, HONG Hua-shan, LIN Xiao-hong, LIN Jun-hua, FANG Mei-qin. Expression of TRPC in the smooth muscle cells of the small intramyocardial arteries in the left ventricles of spontaneously hypertensive rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2013, 18(8): 879-884.

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