慢性阻塞性肺病稳定期患者外周血Th17/Treg失衡情况及与预后的关系

中国临床药理学与治疗学 ›› 2014, Vol. 19 ›› Issue (12): 1383-1388.

慢性阻塞性肺病稳定期患者外周血Th17/Treg失衡情况及与预后的关系

陈雪松, 吴宁, 齐玉琴, 张兴虎, 万文辉, 钱晓明

南京军区南京总医院干部病房一科,南京军区老年病研究中心,南京 210002,江苏

收稿日期:2014-04-11 修回日期:2014-09-09 发布日期:2020-07-20
通讯作者: 钱晓明,通信作者,女,硕士,主任医师,研究方向:老年疾病治疗与管理。Tel:025-80860433 E-mail:Qxm1954@163.com
作者简介:陈雪松,男,硕士,住院医师,研究方向:老年呼吸疾病。Tel:025-80860433 E-mail:lancetchen@163.com
基金资助:
南京军区南京总医院院管课题(2012068)

Roles of peripheral Th17/Treg in patients with stable chronic obstructive pulmonary disease

CHEN Xue-song, WU Ning, QI Yu-qin, ZHANG Xing-hu, WAN Wen-hui, QIAN Xiao-ming

Department of Geriatrics, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, Jiangsu, China

Received:2014-04-11 Revised:2014-09-09 Published:2020-07-20

摘要/Abstract

摘要： 目的: 观察慢性阻塞性肺病(chronic obstructive pulmonary disease,COPD)稳定期患者外周血中Th17与Treg细胞的水平。方法: 选取本院2010年1月-2012年12月间确诊的40例COPD稳定期患者,选取同期40例健康体检健康人群作为对照组,收集两组人群的外周血后采用流式细胞术检测各个人群Th17与Treg细胞亚群的比例,采用ELISA法检测血清中的IL-17、TGF-β水平,采用RT-PCR检测转录因子RORγT与Foxp3 mRNA水平。结果: COPD稳定期患者外周血中Th17比例为(3.14±0.31)%,显著高于对照组人群的(1.23±0.24)%,差异有统计学意义(P<0.05);COPD稳定期患者外周血中Treg比例为(6.27±0.32)%,显著高于对照组人群的(3.43±0.32)%,差异有统计学意义(P<0.01);COPD稳定期患者外周血中IL-17水平为(89.36±4.13) pg/mL,显著高于对照组的(30.35±2.53) pg/mL,差异有统计学意义(P<0.05);COPD稳定期患者外周血中TGF-β水平为(681.45±41.65) pg/mL,显著高于对照组人群的(519.36±30.43) pg/mL,差异有统计学意义(P<0.01);COPD稳定期患者外周血转录因子Foxp3与RORγT mRNA水平均显著高于对照组人群,差异有统计学意义(均P<0.01)。COPD稳定期患者Th17、Treg比例高水平的住院天数和病死率均高于对应低水平亚组(P<0.05)。结论: Th17、Treg细胞及IL-17、TGF-β水平在COPD稳定期患者外周血表达增加,且转录因子Foxp3与RORγT mRNA水平表达增加,提示其与COPD患者的预后有关,其外周血中Th17与Treg细胞的不平衡可能参与了COPD发病和病程的进展。

关键词: 慢性阻塞性肺病, Th17, Treg, IL-17, TGF-β

Abstract: AIM: To observe the level of Th17 and Treg cells in peripheral blood of stable chronic obstructive plumonary disease(COPD) patients. METHODS: Froty patients that were diagnosed as COPD were selected as expperiment group from January 2010 to December 2012, another 40 healthy people were selected as control group at the same period. Peripheral blood was collected from two groups to analyze Th17 and the proportion of Treg cell subset by flow cytometry, to detect serum IL-17, TGF-β by ELISA, and detected the transcription factor Foxp3 and RORγT mRNA by RT-PCR. RESULTS: The percentage of Th17 in peripheral blood of SCOPD patients was (3.14±0.31)%, significantly higher than (1.23±0.24)% in control group, the difference was statistically significant (P<0.05); proportion of Treg in peripheral blood of SCOPD patients was (6.27±0.32)%, significantly higher than that in the control group (3.43±0.32) %, the difference was statistically significant (P<0.01); SCOPD patients with levels of IL-17 (89.36±4.13) pg/mL, significantly higher than the control group (30.35±2.53) pg/mL, the difference was statistically significant (P<0.05); SCOPD patients with the TGF-β levels (681.45±41.65) pg/mL, significantly higher than the population of control group (519.36±30.43) pg/mL, the difference there was statistically significant (P<0.01); SCOPD patients with transcription factor Foxp3 and RORγT mRNA levels were significantly higher the control group, the difference was statistically significant (all P<0.01). CONCLUSION: Th17, Treg cells and IL-17, TGF-β levels in SCOPD patients increased and the transcription factor Foxp3 and RORγT mRNA expression levels also increased. They played an important role in clinical research.

Key words: COPD, Th17, Treg, IL-17, TGF-β

中图分类号:

R563.5

陈雪松, 吴宁, 齐玉琴, 张兴虎, 万文辉, 钱晓明. 慢性阻塞性肺病稳定期患者外周血Th17/Treg失衡情况及与预后的关系[J]. 中国临床药理学与治疗学, 2014, 19(12): 1383-1388.

CHEN Xue-song, WU Ning, QI Yu-qin, ZHANG Xing-hu, WAN Wen-hui, QIAN Xiao-ming. Roles of peripheral Th17/Treg in patients with stable chronic obstructive pulmonary disease[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(12): 1383-1388.

参考文献

[1] Jarad N. Chronic obstructive pulmonary disease (COPD) and old age [J]. Chron Respir Dis, 2011, 8(2): 143-151.
[2] Liu LL, Qin Y, Cai JF, et al. Th17/Treg imbalance in adult patients with minimal change nephrotic syndrome[J]. Clin Immunol, 2011, 139(3): 314-320.
[3] Ratajczak P, Janin A, Peffault de Latour R, et al. Th17/Treg ratio in human graft-versus-host disease[J]. Blood, 2010, 116(7): 1165-1171.
[4] Li J, Lai X, Liao W, et al. The dynamic changes of Th17/Treg cytokines in rat liver transplant rejection and tolerance[J]. Int Immunopharmacol, 2011, 11(8): 962-967.
[5] 中华医学会呼吸病学分会慢性阻塞性肺疾病组. 慢性阻塞性肺疾病诊治指南(修订版)[J]. 中华内科杂志, 2007, 46(3): 254-261.
[6] Li N, Bian H, Zhang J, et al The Th17/Treg imbalance exists in patients with heart failure with normal ejection fraction and heart failure with reduced ejection fraction[J]. Clin Chim Acta, 2010, 411(23/24): 1963-1968.
[7] Li Q, Wang Y, Chen K, et al. The role of oxidized low-density lipoprotein in breaking peripheral Th17/Treg balance in patients with acute coronary syndrome[J]. Biochem Biophys Res Commun, 2010, 394(3): 836-842.
[8] Zhang F, Meng G, Strober W. Interactions among the transcription factors Runx1, RORgammat and Foxp3 regulate the differentiation of interleukin 17-producing T cells[J]. Nat Immunol, 2008, 9(11): 1297-1306.
[9] Cosio MG, Saetta M, Agusti A. Immunologic aspects of chronic obstructive pulmonary disease[J]. N Engl J Med, 2009, 360(23): 2445-2454.
[10] Chatila TA. Role of regulatory T cells in human diseases[J]. J Allergy Clin Immunol, 2005, 116(5): 949-959.
[11] Vargas-Rojas MI, Ramirez-Venegas A, Limon-Camacho L, et al Increase of Th17 cells in peripheral blood of patients with chronic obstructive pulmonary disease[J]. Respiratory Medicine, 2011, 105(11): 1648-1654.
[12] Barczyk A, Pierzchala W, Sozanska E. Interleukin-17 in sputum correlates with airway hyper responsiveness to methacholine [J]. Respir Med, 2003, 97(9): 726-733.
[13] 石建邦,徐晓玲,夏淮玲,等.慢性阻塞性肺疾病稳定期患者和肺功能正常吸烟者外周血中Th17细胞的表达及意义[J].安徽医科大学学报,2012,47(7):835-838.
[14] 陈钢,谢灿茂,罗志扬,等. 慢性阻塞性肺疾病加重期和稳定期Th17 和Treg 细胞的表达[J].中山大学学报(医学科学版),2013,34(6):917-921.
[15] Miossec P.Interleukin-17 in rheumatoid arthritis: If T cells were to contribute to inflammation and destruction through synergy[J].Arthritis Rheum,2003,48(3) : 594-601.
[16] Domagala-Kulawik J, Hoser G, Dabrowska M, et al. CD₄⁺/CD₂₅⁺ cells in systemic inflammation in COPD[J]. Scand J Immunol, 2011, 73(1): 59-65.