丹参与电针对脑缺血再灌注损伤大鼠GFAP和iNOS表达的影响

中国临床药理学与治疗学 ›› 2014, Vol. 19 ›› Issue (2): 127-132.

丹参与电针对脑缺血再灌注损伤大鼠GFAP和iNOS表达的影响

张业贵¹, 赵健¹, 丁艳霞¹, 侯良芹²

¹皖南医学院解剖学教研室,芜湖 241002,安徽;
²安徽中医药大学解剖学教研室,合肥230038,安徽

收稿日期:2013-11-12 修回日期:2014-02-26 出版日期:2014-02-26 发布日期:2014-03-31
通讯作者: 侯良芹,女,博士,副教授,硕士生导师,研究方向:神经生物学。Tel: 0551-65169215 E-mail: hlq1227@sohu.com
作者简介:张业贵,男,硕士,讲师,研究方向:神经生物学。Tel: 13855346546 E-mail: zhangyegui025@163.com
基金资助:
安徽省高校省级优秀青年基金项目(2009SQRZ186);安徽省自然科学基金项目(11040606M162)

Effects of salvia miltiorrhiza and electroacupuncture on the expressions of glial fibrilliary acidic protein and iNOS in rats with cerebral ischemia-reperfusion injury

ZHANG Ye-gui¹, ZHAO Jian¹, DING Yan-xia¹, HOU Liang-qin²

¹Department of Anatomy, Wannan Medical College, Wuhu 241002,Anhui, China;
²Department of Anatomy, Anhui University of Traditional Chinese Medicine, Hefei 230038, Anhui, China

Received:2013-11-12 Revised:2014-02-26 Online:2014-02-26 Published:2014-03-31

摘要/Abstract

摘要： 目的: 观察脑缺血再灌注损伤后大鼠脑胶质原纤维酸性蛋白(GFAP)及诱导型一氧化氮合酶(iNOS)的表达,以及丹参与电针对其表达的影响。方法: 将60只SD大鼠随机分为假手术组、模型组、丹参组、电针组、丹参+电针组,采用大脑中动脉线栓法建立脑缺血再灌注模型。造模后,丹参组给予丹参注射液 5 g/kg 腹腔注射,电针组刺激“百会”、“大椎”穴,疏密波,频率2~15 Hz,持续 30 min,丹参+电针组给予丹参注射液腹腔注射和电针,各组治疗均每天1次,连续 4 d。进行神经功能缺损评分、干湿重法测脑组织含水量,采用HE染色观察脑组织的病理形态学变化,免疫组织化学染色法检测各组大鼠纹状体GFAP、iNOS的表达。结果: 模型组神经功能缺损评分和脑组织含水量均明显高于假手术组(P<0.01);与模型组比较,丹参组、电针组、丹参+电针组神经功能缺损评分和脑组织含水量均显著降低(P<0.05 或P<0.01),并且丹参+电针组的评分和含水量显著低于丹参组、电针组 (P<0.05 或P<0.01);HE染色显示的病理形态学改变与上述一致。模型组GFAP、iNOS的表达均明显高于假手术组 (P<0.01);与模型组比较,丹参组、电针组、丹参+电针组GFAP、iNOS的表达均明显降低(P<0.05 或P<0.01),并且丹参+电针组的表达明显低于丹参组、电针组(P<0.05 或P<0.01)。结论: 丹参和电针对脑缺血再灌注损伤有神经保护作用,可能与其抑制星型胶质细胞的过度活化,降低iNOS的表达有关,且二者合用效果更佳。

关键词: 脑缺血再灌注, 电针, 胶质原纤维酸性蛋白, 诱导型一氧化氮合酶

Abstract: AIM: To observe the effects of salvia miltiorrhiza(SM) and electroacupuncture (EA) on the expressions of glial fibrilliary acidic protein (GFAP) and iNOS in rats after focal cerebral ischemia-reperfusion.METHODS: 60 Sprague-Dawley rats were randomized into the sham opration, model, SM, EA, and SM+EA groups. The models rats received intraluminal middle cerebral artery occlusion(MCAO) with a nylon monofilament suture. SM injection at 5 g/kg were injected into abdominal cavity in the SM group, EA(2-15Hz, dilatational wave) at Baihui and Dazhui was performed to rats in the EA group, lasting for 30 min, SM+EA group was treated with both SM abdominal cavity injection and electroacupuncture therapy, once a day for 4 days.To observe the neurologic impairment score in each group, and the water content of brain tissue was determined by weighing. HE staining was performed to display the pathological changes of the brain tissues, and the expressions of GFAP and iNOS in striatum were detected by immunohistochemical method.RESULTS: The neurologic impairment score and water content of brain tissue in model group were significantly increased compared with sham operation group(P<0.01).Compared with model group, the neurologic impairment score and water content of brain tissue in SM group, EA group and SM+EA group were significantly decreased (P<0.05,P<0.01), and the score and water content in SM+EA group was obviously lower than those of the SM group and the EA group(P<0.05, P<0.01). The pathological changes of the brain tissues of four group rats were consistent with the above result. The expression of GFAP and iNOS in model group was obviously increased compared with sham operation group(P<0.01).Compared with model group, the expression of GFAP and iNOS in SM group, EA group and SM+EA group were significantly decreased (P<0.05, P<0.01). The expression of GFAP and iNOS in SM+EA group was obviously lower than those of the SM group and the EA group (P<0.05, P<0.01).CONCLUSION: SM and EA has a neuroprotective activity in cerebral ischemia-reperfusion neuronal injury, which is probably related to inhibit the excessive activation of astrocytes, decrease the expression of iNOS, besides, the effects of SM combined with EA was higher than the SM group or the EA group.

Key words: cerebral ischemia reperfusion, electroacupuncture, glial fibrilliary acidic protein, iNOS

中图分类号:

R965.2

张业贵, 赵健, 丁艳霞, 侯良芹. 丹参与电针对脑缺血再灌注损伤大鼠GFAP和iNOS表达的影响[J]. 中国临床药理学与治疗学, 2014, 19(2): 127-132.

ZHANG Ye-gui, ZHAO Jian, DING Yan-xia, HOU Liang-qin. Effects of salvia miltiorrhiza and electroacupuncture on the expressions of glial fibrilliary acidic protein and iNOS in rats with cerebral ischemia-reperfusion injury[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(2): 127-132.

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