指数富集的配体系统进化技术的发展及其在临床治疗中的应用

中国临床药理学与治疗学 ›› 2014, Vol. 19 ›› Issue (2): 228-232.

指数富集的配体系统进化技术的发展及其在临床治疗中的应用

龙宇鹏, 任君

中国人民解放军第324医院检验科,重庆 400020

收稿日期:2013-07-27 修回日期:2014-02-19 出版日期:2014-02-26 发布日期:2014-03-31
通讯作者: 任君,男,副主任技师,临床检验新技术及临床输血治疗。E-mail: rj73@sohu.com
作者简介:龙宇鹏,女,硕士,主管技师,研究方向:临床检验新技术及临床输血治疗。E-mail: lyp_emily@126.com
基金资助:
重庆市卫生局医学科研计划项目(面上项目,2011-2-590)

The development of the SELEX technology and its application in clinical treatment

LONG Yu-peng, REN Jun

Department of Clinical laboratory, the 324th Hospital of PLA, Chongqing 400020, China

Received:2013-07-27 Revised:2014-02-19 Online:2014-02-26 Published:2014-03-31

摘要/Abstract

摘要： 基于作为配基的寡核苷酸的三维立体结构,指数富集的配体系统进化(SELEX)技术能够筛选获得高效、特异结合靶分子的寡核苷酸适体。该配基由于其亲和力高、特异性强、免疫原性低、筛选周期短及适用范围广等特性,在临床诊断和疾病治疗中拥有多项优势,具有被研制成新型适体药物应用于临床的巨大潜力。本文从SELEX技术的发展、配基的修饰改造、临床治疗的优势、配基作为靶蛋白功能阻断剂和药物分子靶向载体进行的临床应用等几个方面,对寡核苷酸适体在临床疾病治疗中的应用进行综述。

关键词: 指数富集的配体系统进化, 寡核苷酸适体, 配基, 治疗

Abstract: Based on the three-dimensional structure of oligonucleotide ligands, systematic evolution of ligands by exponential enrichment (SELEX) technology can screen for efficient and specific oligonucleotide aptamer to combine with molecular targets. Due to its high affinity, strong specificity, low immunogenicity, short filtering cycle and wide range of application, the ligands have several advantages and potential for new drugs in clinical diagnosis and treatment. In this article the application of oligonucleotide aptamer in the clinical treatment are reviewed from several aspects such as the development of the SELEX technology, modification of ligands, the advantages of clinical treatment, functions of ligands as target protein blocking agents and targeted drug delivery carrier.

Key words: SELEX, oligonucleotide aptamer, ligands, treatment

中图分类号:

R373

龙宇鹏, 任君. 指数富集的配体系统进化技术的发展及其在临床治疗中的应用[J]. 中国临床药理学与治疗学, 2014, 19(2): 228-232.

LONG Yu-peng, REN Jun. The development of the SELEX technology and its application in clinical treatment[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(2): 228-232.

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