高糖饮水诱导代谢综合征小鼠模型的实验研究

中国临床药理学与治疗学 ›› 2014, Vol. 19 ›› Issue (9): 1011-1015.

高糖饮水诱导代谢综合征小鼠模型的实验研究

沈纪中, 柳航

南京大学医学院附属鼓楼医院药学部,南京 210008,江苏

收稿日期:2014-05-09 修回日期:2014-09-02 出版日期:2014-09-26 发布日期:2014-09-26
作者简介:沈纪中,男,博士,主管药师,研究方向:临床药理学。 Tel: 025-83105670 E-mail: sjzxlf@163.com
基金资助:
国家自然科学基金项目(81302817); 江苏省六大人才高峰计划项目(WSN-027); 南京药学会常州四药科研项目(2013YX006)

Experimental study of a metabolic syndrome mouse model induced by high sucrose-containing drinking water

SHEN Ji-zhong, LIU Hang

Department of Pharmacy, Drum Tower Hospital Affiliated to Medical School of Nanjing University, Nanjing 210008, Jiangsu,China

Received:2014-05-09 Revised:2014-09-02 Online:2014-09-26 Published:2014-09-26

摘要/Abstract

摘要： 目的通过长期喂饮32%蔗糖水来建立模拟人类代谢综合征(MetS)的小鼠动物模型。方法 3周龄昆明种小鼠,随机分成对照组(Control)和糖水组(SW),雌雄各半,每组10只。所有小鼠均自由取食常规饲料。对照组小鼠饮用自来水,糖水组小鼠饮用含32%蔗糖的糖水。饲养至24周龄时,行口服葡萄糖耐量实验(OGTT),记录小鼠体质量、腰围、体长,取禁食血液分离血清,检测各组小鼠的血生化指标。后宰杀,分离不同部位脂肪组织,称重并记录。结果与对照组小鼠比较,SW小鼠表现出向心性肥胖体征。SW小鼠也出现了糖脂代谢紊乱。OGTT实验结果提示:SW小鼠的糖处理能力也出现了明显的下降。胰岛素代谢方面,SW小鼠出现了高胰岛素血症和胰岛素抵抗。结论高糖饮水诱导MetS小鼠模型较好地模拟了人类MetS的病理生理特征。造模条件要求低,操作易掌握,且造模经济成本低,是一种较理想的MetS动物模型,可为抗MetS药物的药效学研究提供理想平台。

关键词: 代谢综合征, 肥胖, 胰岛素抵抗, 高血糖

Abstract: AIM: To establish a mouse model with metabolic disorders similar to human metabolic syndrome (MetS) by induced with long-term 32% high sucrose-containing drinking water. METHODS: All male/female mice (3 weeks of age) were divided into two different groups randomly (n=10):control group and sucrose water (SW) group. Control group was received chow diet plus plain water, while SW group were received chow diet plus 32% sucrose water. At 24 weeks of age, oral glucose tolerance test (OGTT) were conducted to assess glucose handling ability of mice. Then, body weight, body length and waist circumference was measured in live mice, blood samples were collected from the orbital vein of experimental animals for measuring blood biochemical index. Then, mice were sacrificed and different parts of the adipose tissue were weighted and recorded. RESULTS: Compared with control mice, SW mice emerged a profound central obesity body characteristics. Based on the SW mice blood biochemical index examination, we found that SW mice have emerged glucose and lipid metabolism disorder. OGTT results suggest that: glucose processing capabilities of SW mice has shown a significant decline.For insulin metabolism, SW mice had significant hyperinsulinemia and insulin resistance.CONCLUSION: High sucrose water-induced MetS mice model well mimic the pathophysiological characteristics of MetS in humans. It is a readily available and low-cost animal model of MetS, and will provide a platform for the new therapeutic drugs of MetS.

Key words: metabolic syndrome, obesity, insulin resistance, hyperglycemia

中图分类号:

R965.2

沈纪中, 柳航. 高糖饮水诱导代谢综合征小鼠模型的实验研究[J]. 中国临床药理学与治疗学, 2014, 19(9): 1011-1015.

SHEN Ji-zhong, LIU Hang. Experimental study of a metabolic syndrome mouse model induced by high sucrose-containing drinking water[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(9): 1011-1015.

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