乙醇诱导人脐静脉内皮细胞凋亡及对ICAM-1蛋白表达影响的研究

中国临床药理学与治疗学 ›› 2015, Vol. 20 ›› Issue (3): 266-268.

乙醇诱导人脐静脉内皮细胞凋亡及对ICAM-1蛋白表达影响的研究

何胜虎^1,2, 周胜华¹, 张晶², 王雪飞², 王大新²

¹中南大学湘雅二院,长沙 420011,湖南;
²江苏省苏北人民医院心内科,扬州 225001,江苏

收稿日期:2014-10-20 修回日期:2015-01-30 发布日期:2015-04-08
作者简介:周胜华,男,主任医师,教授,研究方向:心血管内科。Tel: 13707483674 E-mail: zhougq@21cn.com

Effect of human umbilical vein endothelial cells apoptosis induced by ethanol and the expression of ICAM-1

HE Sheng-hu^1,2, ZHOU Sheng-hua¹, ZHANG Jing², WANG Xue-fei², WANG Da-xin²

¹Second Xiangya Hospital, Central South University, Changsha 410011,Hunan, China;
²Department of Cardiovascular Medicine, Northern Jiangsu People's Hospital, Medical College of Yangzhou University, Yangzhou 225001, Jiangsu, China

Received:2014-10-20 Revised:2015-01-30 Published:2015-04-08

摘要/Abstract

摘要： 目的: 研究乙醇体外诱导人脐静脉内皮细胞(HUVEC)凋亡,及对细胞间黏附分子(ICAM-1)蛋白表达的影响。方法: 以不同浓度乙醇(50、100和 200 mmol/L)作用于体外培养的人脐静脉内皮细胞 24 h,四甲基偶氮唑盐(MTT)比色法测定各组细胞活力;流式细胞仪检测细胞凋亡率;酶联免疫吸附法检测ICAM-1蛋白表达。结果: 乙醇能抑制内皮细胞活力,促进细胞凋亡,增加ICAM-1表达,并呈浓度依赖性。结论: 乙醇能诱导内皮细胞损伤,其机制可能与促进细胞凋亡、影响ICAM-1蛋白表达有关。

关键词: 乙醇, 人脐静脉内皮细胞, 细胞间黏附分子, 细胞凋亡

Abstract: AIM: To study the apoptosis induced by ethanol on human umbilical vein endothelial cells(HUVEC)and the expression of ICAM-1 in vitro.METHODS: HUVEC were treated with different concentrations of ethanol (50,100 and 200 mmol/L) for 24 hours, the activity of cells was determined by methyl thiazolyl tetrazolium (MTT) assay; the rate of apoptosis was detected by flow cytometry (FCM) assay; and the expression of ICAM-1 by enzyme linked immunosorbent assay.RESULTS: Ethanol can inhibit the cells activity, promote the apoptosis, increase the expression of ICAM-1 of HUVEC in dose-dependent manner.CONCLUSION: Ethanol can damage endothelial cells and its possible mechanism may be linked to promote the apoptosis and the expression of ICAM-1.

Key words: ethanol, HUVEC, ICAM-1, apoptosis

中图分类号:

R965.2

何胜虎, 周胜华, 张晶, 王雪飞, 王大新. 乙醇诱导人脐静脉内皮细胞凋亡及对ICAM-1蛋白表达影响的研究[J]. 中国临床药理学与治疗学, 2015, 20(3): 266-268.

HE Sheng-hu, ZHOU Sheng-hua, ZHANG Jing, WANG Xue-fei, WANG Da-xin. Effect of human umbilical vein endothelial cells apoptosis induced by ethanol and the expression of ICAM-1[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2015, 20(3): 266-268.

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