H2S在大鼠油酸性肺水肿中的作用及机制研究

中国临床药理学与治疗学 ›› 2015, Vol. 20 ›› Issue (3): 279-283.

H₂S在大鼠油酸性肺水肿中的作用及机制研究

李伟¹, 陆晓华¹, 姜玉新², 王国光¹

¹皖南医学院基础医学院病理生理学教研室, ²生理学教研室,芜湖 241002,安徽

收稿日期:2014-03-27 修回日期:2014-10-23 发布日期:2015-04-08
通讯作者: 王国光,男,博士,副教授,研究方向:糖尿病及其并发症防治。Tel: 0553-3932476 E-mail: guoguangw1226@sina.com
作者简介:李伟,男,硕士,讲师,研究方向:气体信号分子与危重病治疗。
基金资助:
国家自然科学基金资助课题(81172790) ;军队重点项目(BJN14C001)

The effect and mechanism research of H₂S in rats with pulmonary edema induced by oleic acid

LI Wei¹, LU Xiao-hua¹, JIANG Yu-xin², WANG Guo-guang¹

¹Department of Pathophysiology,Wannan Medical College, ²Department of Physiology, Wuhu 241002, Anhui, China

Received:2014-03-27 Revised:2014-10-23 Published:2015-04-08

摘要/Abstract

摘要： 目的: 研究H₂S对油酸导致的大鼠肺水肿的作用及其机制。方法: 32只SD大鼠平均分成4组:对照组(CG组)、模型组(MG组)、预防组(PG组)、NaHS组(SG组)。CG组尾静脉注射生理盐水 0.2 mL/100 g 后,再注射生理盐水 7 μL/100 g。MG组尾静脉注射生理盐水 0.2 mL/100 g 后,再注射油酸 7 μL/100 g。PG组尾静脉注射NaHS 3.4 mg/kg 后,再注射油酸 7 μL/100 g。SG组尾静脉注射NaHS 3.4 mg/kg 后,再注射生理盐水 7 μL/100 g。各组大鼠注射药物 40 min 后颈总动脉取血并处死,取肺脏。观察肺脏大体观及肺组织病理改变,计算肺组织湿干重比,测定血清及肺组织丙二醛(MDA)水平、超氧化物歧化酶(SOD)活力。结果: 与CG组相比,MG组大鼠肺脏包膜下淤血严重,肺脏表面大量片状出血、灶性实变及梗死;肺泡间隔增宽,肺泡壁损伤、断裂,肺泡内存在大量粉红色液体及圆形气泡;肺湿干重比(P<0.05)及血清、肺组织中MDA水平均明显增加(P<0.01),血清与肺组织SOD活力显著降低(P<0.01)。与MG组相比,PG组大鼠肺脏仅见肺底部仍存在片状出血;肺组织病理结构显示间质轻度增厚,部分肺泡内存在粉红色液体,肺泡内圆形气泡少见,肺脏水肿程度较轻,湿干重比减小(P<0.05);血清与肺组织中MDA水平降低(P<0.01),而血清与肺组织中SOD活力则明显增高(P<0.01)。与CG组比较,SG组各项指标差异均无统计学意义。结论: H₂S能显著减轻肺组织损伤,减少血浆渗出量,具有减轻肺水肿的作用,其机制可能与增强机体的抗氧化能力,减轻肺组织的氧化损伤以及降低血管压力有关。

关键词: H₂S, 油酸, 肺水肿

Abstract: AIM: To study the effect and mechanism of H₂S in rats with pulmonary edema induced by oleic acid.METHODS: Thirty two rats were randomly divided into four groups: control group (CG) ,the rats ware injected with normal saline 0.2 mL/100 g intravenously followed by intravenous of normal saline 7 uL/100 g. Model group (MG) ,the rats were injected normal saline 0.2 mL/100 g intravenously, then injected with oleic acid 7 uL /100 g. Prevention group(PG), the rats were injected with NaHS 3.4mg/kg, then injected by equal amount of oleic acid intravenously and NaHS group(SG) ,the rats were injected with NaHS 3.4 mg/kg, then injected normal saline 7 uL /100 g intravenously. After 40 min, the rats were killed. blood and lung were got for analyzing the level of MDA and the activity of SOD, observing lung pathologic gross and pathologic changes, calculating wet and dry weight ratioRESULTS: Compared with the CG group,flaky bleeding was emerged in the lung surface,focal consolidation and infarction and subcapsular congestion seriously in MG group.Oleic acid increased the thickness of alveolar septal, damaged the alveolar wall, and made alveoli full of pink secretion and round bubbles. Moreover, W/D and the level of MDA in blood plasma and lung tissue in MG group were increased obviously compared with those in CG group. But the activity of SOD decreased significantly. Lung surface only emerged flaky bleeding on the lower lung fields, slight interstitial thickening, minority alveolar exited pink secretion in PG group. W/D and the level of MDA in plasma and lung tissue in PG group were decreased obviously compared with those in MG group. The activity of SOD was increased significantly in PG group. Meanwhile, indicators of SG group have no significant differences.CONCLUSION: H₂S has the effects of alleviating the lung injury, decreasing plasma exudation, and mitigating pulmonary edema. The mechanism may be correlation with enhancing the antioxidation, alleviating oxidative damage of lung tissue and lowering blood pressure.

Key words: H₂S, oleic acid, pulmonary edema

中图分类号:

R965.2

李伟, 陆晓华, 姜玉新, 王国光. H₂S在大鼠油酸性肺水肿中的作用及机制研究[J]. 中国临床药理学与治疗学, 2015, 20(3): 279-283.

LI Wei, LU Xiao-hua, JIANG Yu-xin, WANG Guo-guang. The effect and mechanism research of H₂S in rats with pulmonary edema induced by oleic acid[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2015, 20(3): 279-283.

参考文献

[1] 赵波,赵进军.急性肺水肿的研究现状[J].心血管病学进展,2007,28(3):445-448.
[2] 李锐,黄宇光.输血相关急性肺损伤的免疫机制[J].中国临床药理学与治疗学,2013,18(5):575-579.
[3] Geng B, Yang J, Qi Y, et al. H₂S generated by heart in rat and its effects on cardiac function[J]. Biochem Biophys Res Commun, 2004, 313(2):362-368.
[4] Yan H, Du J, Tang C. The possible role of hydrogen sulfide on the pathogenesis of spontaneous hypertension in rats[J]. Biochem Biophys Res Commun,2004, 313 (1):22-27.
[5] Chunyu Z, Junbao D, Dingfang B, et al. The regulatory effect of hydrogen sulfide on hypoxic pulmonary hypertension in rats[J]. Biochem Biophys Res Commun,2003, 302 (4):810-816.
[6] 祁玉龙.高原性肺水肿58 例临床治疗体会[J].中国临床研究,2013,26(11):1181-1182.
[7] Chow CW, Abreu MTH, Suzuki T, et al. Oxidative stress and acute lung injury[J]. Am J Respir Cell Mol Biol, 2003, 29(4):427-431.
[8] 梅双,赵金垣,温韬,等.油酸性急性肺损伤启动机制的实验研究[J].中国工业医学杂志,2007,20(2):67-71.
[9] 蒋柳明, 金建国, 祝卿,等.异丙酚对油酸型急性肺损伤兔血清TNF-A、IL-1B水平的影响[J].中国临床药理学与治疗学,2011,16(9):1002-1005.
[10]周辉,高洪,严玉霖,等.内源性气体信号分子H₂S 研究进展[J].动物医学进展,2009,30(6):94-97.
[11]李康琪, 张广钦.硫化氢对中枢神经系统作用的研究进展[J].中国临床药理学与治疗学,2010,15(10):1183-1188.
[12]Du J, Hui Y, Cheung Y, et al. The possible role of hydrogen sulfide as a smooth muscle cell proliferation inhibitor in rat cultured cells[J]. Heart Vessels,2004, 19(2):75-80.
[13]张天骄,李悦.硫化氢与心血管系统离子通道[J].中国心脏起搏与心电生理杂志,2013,27(3):266-268.
[14]孟祥艳,鲁平,凌亦凌,等.气体信号分子硫化氢在脂多糖诱导大鼠肺急性炎症反应中的作用[J].河北医科大学学报,2005,26(2):132-134.
[15]杨雪,刘洁,吴晓明,等.H₂S气体分子及相关药物研究进展[J].药学与临床研究,2014,22(2):128-133.

H₂S在大鼠油酸性肺水肿中的作用及机制研究

The effect and mechanism research of H₂S in rats with pulmonary edema induced by oleic acid

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