关键词: 保肝解毒颗粒, 免疫性肝损伤, 炎症因子, 紧密连接蛋白, 肠黏膜屏障

Abstract:

AIM: To investigate the influence of Jiedu hugan granule on the inflammatory factor and tight junction protein of small intestine in rats with immunological liver injury.  METHODS: Fifty SD rats were randomly divided into control group, model group, low, middle and high experimental group of Jiedu hugan granule (2.7, 5.4, and 10.8 g/kg). All the groups excepted the control group received intraperitoneal injections of 0.5 mL pig serum for each to establish immunological liver injury model. The experimental group was irrigated 1time/d, continuous dosing for 14 d. The liver tissue and small intestinal tissue pathological changes were observed by HE staining, the serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) were detected, and the expression of liver tissue TNF-α and small intestine tissue occludin-5 were detected by immunohistochemical method. RESULTS:The model group liver tissue showed accumulation of fat cells, liver cells enlargement, disorganized liver and associated with inflammatory cells infiltration; the experimental group liver cell morphological ruled, hepatic cords in alignment and inflammatory cells infiltration significantly decrease. The model group small intestinal mucosa villi showed atrophy, fall off of epithelial cell, edema, large number of inflammatory cells infiltration; the experimental group showed closely-knitted small intestinal mucosa villi, relieve edema, and no obvious infiltration of inflammatory cells. The levels of ALT, AST, LDH and TNF-α in experimental group were statistically lower than the model group, and the levels were statistically reduce with the dose of jiedu hugan granule increased. The levels of occludin-5 in experimental group were statistically highter than those of the model group, and the levels of occludin-5 were statistically increase with the dose of Jiedu hugan granule increased, the differences were statistically significant (P<0.05). CONCLUSION: Jiedu hugan granule can improve the organizational structure of liver and small intestine, protect the damage of liver, reduce inflammation, and maintain small intestine mucosal barrier function.

Key words: Jiedu hugan granule, immunological liver injury, inflammation factors, tight junction protein, intestinal mucosal barrier