姜黄素对肝肺综合征大鼠的保护作用

中国临床药理学与治疗学 ›› 2017, Vol. 22 ›› Issue (7): 738-742.

姜黄素对肝肺综合征大鼠的保护作用

李旭炯¹，刘安²，陈云霞³，李淑芬¹

¹长治医学院生理学教研室，长治 046000，山西；²中国中医科学院中药研究所，北京 100700；³长治医学院微生物学教研室，长治 046000，山西

收稿日期:2016-08-03 修回日期:2016-11-07 出版日期:2017-07-26 发布日期:2017-07-19
作者简介:李旭炯，男，副教授，主要从事肝脏病变及其并发症的研究。 E-mail：qazw3000@126.com
基金资助:
科技部重大新药创制重大专项（2014ZX09304307-001）；长治医学院科技创新团队项目（CX201509）

Curcumin alleviate intrapulmonary inflammation in rat experimental hepatopulmonary syndrome

LI Xujiong¹, LIU An ², CHEN Yunxia³, LI Shufen ¹

¹Physiology Department of Changzhi Medical College, Changzhi 046000, Shanxi, China; ² Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China; ³ Microbiology Department of Changzhi Medica l College, Changzhi 046000, Shanxi, China

Received:2016-08-03 Revised:2016-11-07 Online:2017-07-26 Published:2017-07-19

摘要/Abstract

摘要：

目的: 观察姜黄素对胆总管结扎诱导的大鼠肝肺综合征（HPS）肺组织炎性损伤的保护作用。方法: 造模1周后，姜黄素（50 mg·kg^-1·d^-1）通过灌胃给予。给药2周后，进行血气分析、血浆TNF-α及肝功能检测。对肺、肝组织病理改变（HE染色）进行观察。并采用免疫荧光染色对肺组织CD68、NF-κB P-65及TNF-α进行检测，同时采用Western blotting法对IκBα/p-IκBα进行检测。结果: 姜黄素减轻HPS大鼠的肺组织巨噬细胞聚集及炎性损伤、降低了AaPO2,同时也降低了HPS大鼠血浆和肺组织TNF-α含量及NF-κB激活程度。结论: 姜黄素对HPS大鼠的肺组织炎性损伤具有保护作用，其机制可能与NF-κB激活程度减轻有关。

关键词: 肝肺综合征, 姜黄素, TNF-α, NF-κB

Abstract:

AIM: To evaluate the protective effect of curcumin in an experimental model of hepatopulmonary syndrome (HPS) induced by bile duct ligation in rats. METHODS: One week after surgery, curcumin (50 mg·kg^-1·d^-1) was administered intragastrically for two weeks. Arterial blood gas analysis was performed. Plasma TNF-α and ALT level was measured. Pathological change in rat lung tissue and liver tissue were observed through HE staining. CD68, TNF-α and NF-κB P-65 were detected by immunofluorescence, and IκBα/p-IκBα was assessed by Western blotting. RESULTS: Curcumin alleviated lung tissue and pulmonary intravascular monocyte accumulation and significantly decreased AaPO. Also, pulmonary TNF-α level and lung tissue NF-κB activity were reduced. CONCLUSION: Curcumin can protect HPS rat from pulmonary inflammation injury. The mechanism may be related with anti-inflammation by inhibition of TNF-α/NF-κB.

Key words: hepatopulmonary syndrome, curcumin, TNF-α, NF-κB

中图分类号:

R965.2

李旭炯，刘安，陈云霞，李淑芬. 姜黄素对肝肺综合征大鼠的保护作用[J]. 中国临床药理学与治疗学, 2017, 22(7): 738-742.

LI Xujiong, LIU An, CHEN Yunxia, LI Shufen. Curcumin alleviate intrapulmonary inflammation in rat experimental hepatopulmonary syndrome[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(7): 738-742.

参考文献

［1］Pulido-Moran M, Moreno-Fernandez J, Ramirez-Tortosa C, et al. Curcumin and Health［J］. Molecules, 2016,21(3):264.
［2］Hu M, Du Q, Vancurova I, et al. Proapoptotic effect of curcumin on human neutrophils: activation of the p38 mitogen-activated protein kinase pathway［J］. Crit Care Med,2005,33(11):2571-2578.
［3］Ma C, Ma Z, Fu Q, et al. Curcumin attenuates allergic airway inflammation by regulation of CD4+CD25+ regulatory T cells (Tregs)/Th17 balance in ovalbumin-sensitized mice［J］. Fitoterapia, 2013,87:57-64.
［4］Nilani P, Kasthuribai N, Duraisamy B, et al. Invitro antioxidant activity of selected antiasthmatic herbal constituents［J］. AncSci Life, 2009,28(4):3-6.
［5］Thenappan T, Goel A, Marsboom G, et al.A central role for CD68(+) macrophages in hepatopulmonary syndrome. Reversal by macrophage depletion［J］. Am J RespirCrit Care Med, 2011,183(8):1080-1091.
［6］Zhang J, Luo B, Tang L, et al. Pulmonary angiogenesis in a rat model of hepatopulmonarysyndrome［J］. Gastroenterology, 2009,136(3):1070-1080.
［7］Chang CC, Wang SS, Hsieh HG, et al. Rosuvastatin improves hepatopulmonary syndrome through inhibition of inflammatory angiogenesis of lung［J］. ClinSci (Lond), 2015,129(6):449-460.
［8］Liu L, Liu N, Zhao Z, et al. TNF-alpha neutralization improves experimental hepatopulmonary syndrome in rats［J］. Liver Int, 2012,32(6):1018-1026.
［9］Prasad K, Prabhu GK. Image analysis tools for evaluation of microscopic views of immunohistochemically stained specimen in medical research-a review［J］. J Med Syst, 2012,36(4):2621-2631.
［10］Girish V, Vijayalakshmi A. Affordable image analysis using NIH Image/ImageJ［J］. Indian J Cancer, 2004,41(1):47.
［11］Kan S, Zhou H, Jin C, et al. Effects of PDTC on NF-kappaB expression and apoptosis in rats with severe acute pancreatitis-associated lung injury［J］. Int J ClinExp Med, 2015,8(3):3258-3270.
［12］Cho JW, Lee KS, Kim CW. Curcumin attenuates the expression of IL-1beta, IL-6, and TNF-alpha as well as cyclin E in TNF-alpha-treated HaCaT cells; NF-kappaB and MAPKs as potential upstream targets［J］. Int J Mol Med, 2007,19(3):469-474.
［13］Gulcubuk A, Haktanir D, Cakiris A, et al. Effects of curcumin on proinflammatory cytokines and tissue injury in the early and late phases of experimental acute pancreatitis［J］. Pancreatology, 2013,13(4):347-354.

[1]	沈龙德, 李爱禄, 李丽春, 吴银银, 沈龙源, 江秋霞, 贺锦曦, 吴志芬, 余黎吉, 张晓红. 姜黄素对大鼠子宫内膜异位症影响的实验研究#br#[J]. 中国临床药理学与治疗学, 2020, 25(8): 862-868.
[2]	张哲，吕毓，黄攀豪. NF-κB介导的连翘酯苷B对宫颈癌细胞增殖活性的抑制作用[J]. 中国临床药理学与治疗学, 2020, 25(4): 387-392.
[3]	俞陈陈，刘树群，刘建龙，曹红，刘雪娇，蔡茜茜. 姜黄素对高血压病大鼠全脑缺血再灌注时海马神经元损伤及皮质酮、SGK1表达的影响[J]. 中国临床药理学与治疗学, 2019, 24(7): 737-743.
[4]	阮水良，官俏兵. 热休克转录因子2通过HMGB1-TLR4-NF-κB信号促进克罗恩病炎症反应的作用机制研究[J]. 中国临床药理学与治疗学, 2019, 24(4): 369-375.
[5]	邱飞, 高丹, 杨冬梅, 钟小妹, 唐莎, 尹辉明, 张在其. 姜黄素对血小板源性生长因子诱导的血管平滑肌细胞增殖及PTEN表达的影响[J]. 中国临床药理学与治疗学, 2019, 24(12): 1358-1363.
[6]	齐冰丽，黄平，颜瑞雪，李倩. 柚皮素通过PI3K/AKT/NF-κB通路抑制卵巢癌细胞增殖和侵袭、诱导凋亡作用的研究[J]. 中国临床药理学与治疗学, 2019, 24(11): 1234-1241.
[7]	刘小艳，田野，刘骥飞，苏刚，张振昶. 姜黄素对脑缺血再灌注损伤的保护作用机制研究进展[J]. 中国临床药理学与治疗学, 2019, 24(11): 1305-1309.
[8]	赵江涛，梁延鸽. 葛花总黄酮对异丙肾上腺素致大鼠内耳损伤后相关炎性因子影响的实验研究[J]. 中国临床药理学与治疗学, 2018, 23(9): 1003-1007.
[9]	刘静，王媚媚，刘录山，贺卫和，庹勤慧，文红艳. 烟酸姜黄素酯对低剪切应力诱导人脐静脉内皮细胞凋亡的影响及相关机制[J]. 中国临床药理学与治疗学, 2018, 23(8): 855-861.
[10]	方晶晶，陶静，黄钦，史超路，颜碧清，盖磊，厉旭光. 脓毒症中血小板活化对肾小管上皮影响的机制研究[J]. 中国临床药理学与治疗学, 2018, 23(3): 283-289.
[11]	李絮，代谊，黄家凤，温纯洁，邹镇，吴兰香，王果，周宏灏. 姜黄素通过调控组蛋白乙酰化修饰上调乳腺癌MCF-7和MCF-7/DOX细胞中MDR1表达[J]. 中国临床药理学与治疗学, 2017, 22(5): 512-517.
[12]	陈永财, 周斌, 陈雪敏, 翁金月. 姜黄素靶向抑制SW480细胞信号通路研究[J]. 中国临床药理学与治疗学, 2017, 22(5): 526-530.
[13]	张新民，陈鹏，叶盛，夏武杰，李岳春. 蛋白酶体抑制剂减少病毒性心肌炎小鼠炎症因子IL-6和TNF-α的表达[J]. 中国临床药理学与治疗学, 2017, 22(4): 406-411.
[14]	徐步远，潘显阁，陈崇别，蔡敏，徐露. 姜黄素对Lewis肺癌小鼠血管内皮成长因子和endostatin表达的影响[J]. 中国临床药理学与治疗学, 2017, 22(2): 151-154.
[15]	陈丹，林秀贤，陈尧. CYP2E1调控酒精性肝病发病机制的研究进展[J]. 中国临床药理学与治疗学, 2017, 22(2): 198-203.